Production of a mouse hybridoma secreting monoclonal antibody highly specific to hemoglobin Bart's (γ4)
Hemoglobin (Hb) Bart's (γ4) was isolated and purified from Hb Bart's hydrops fetalis syndrome blood by CM-Sephadex C-50 chromatography. The isolated Hb Bart's was analyzed for its purity by high performance liquid chromatography. Balb/c mice were immunized intraperitoneally with Hb Ba...
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th-cmuir.6653943832-617802018-09-11T08:59:07Z Production of a mouse hybridoma secreting monoclonal antibody highly specific to hemoglobin Bart's (γ4) Luksana Makonkawkeyoon Somphon Pharephan Sanit Makonkowkeyoon Medicine Hemoglobin (Hb) Bart's (γ4) was isolated and purified from Hb Bart's hydrops fetalis syndrome blood by CM-Sephadex C-50 chromatography. The isolated Hb Bart's was analyzed for its purity by high performance liquid chromatography. Balb/c mice were immunized intraperitoneally with Hb Bart's. The immunized mouse splenic cells were hybridized with mouse myeloma, X63-Ag8.653, by polyethylene glycol. There were 12 hybridoma clones, out of several thousand culture wells, secreting antibody against purified Hb Bart's. However, when those 12 monoclonal antibodies (mAb) were tested with Hb Bart's (γ 4), HbF (α2γ2), HbH, HbE, and HbA2, there was only 1 hybridoma clone secreting mAb highly reactive to Hb Bart's with very low reactivity to HbF. A rabbit polyclonal antibody with relative high reactivity to Hb Bart's compared to HbF with the ratio of 2.4:1 was also produced by affinity column chromatography for the purpose of developing an enzyme-linked immunosorbent assay (ELISA) base for qualitative and quantitative determination of Hb Bart's in adult hemolysates. Preliminary results in quantitative determination of Hb Bart's in Hb solution of 3 α thalassemia families having at least 1 child with HbH disease and 6 normal subjects indicated that it was possible to quantify Hb Bart's by our developed ELISA with appropriate sensitivity and specificity. © 2006 Carden Jennings Publishing Co., Ltd. 2018-09-11T08:59:07Z 2018-09-11T08:59:07Z 2006-12-04 Journal 10802924 2-s2.0-37849185172 10.1532/LH96.06027 https://www.scopus.com/inward/record.uri?partnerID=HzOxMe3b&scp=37849185172&origin=inward http://cmuir.cmu.ac.th/jspui/handle/6653943832/61780 |
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Medicine Luksana Makonkawkeyoon Somphon Pharephan Sanit Makonkowkeyoon Production of a mouse hybridoma secreting monoclonal antibody highly specific to hemoglobin Bart's (γ4) |
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Hemoglobin (Hb) Bart's (γ4) was isolated and purified from Hb Bart's hydrops fetalis syndrome blood by CM-Sephadex C-50 chromatography. The isolated Hb Bart's was analyzed for its purity by high performance liquid chromatography. Balb/c mice were immunized intraperitoneally with Hb Bart's. The immunized mouse splenic cells were hybridized with mouse myeloma, X63-Ag8.653, by polyethylene glycol. There were 12 hybridoma clones, out of several thousand culture wells, secreting antibody against purified Hb Bart's. However, when those 12 monoclonal antibodies (mAb) were tested with Hb Bart's (γ 4), HbF (α2γ2), HbH, HbE, and HbA2, there was only 1 hybridoma clone secreting mAb highly reactive to Hb Bart's with very low reactivity to HbF. A rabbit polyclonal antibody with relative high reactivity to Hb Bart's compared to HbF with the ratio of 2.4:1 was also produced by affinity column chromatography for the purpose of developing an enzyme-linked immunosorbent assay (ELISA) base for qualitative and quantitative determination of Hb Bart's in adult hemolysates. Preliminary results in quantitative determination of Hb Bart's in Hb solution of 3 α thalassemia families having at least 1 child with HbH disease and 6 normal subjects indicated that it was possible to quantify Hb Bart's by our developed ELISA with appropriate sensitivity and specificity. © 2006 Carden Jennings Publishing Co., Ltd. |
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Luksana Makonkawkeyoon Somphon Pharephan Sanit Makonkowkeyoon |
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Luksana Makonkawkeyoon Somphon Pharephan Sanit Makonkowkeyoon |
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Luksana Makonkawkeyoon |
title |
Production of a mouse hybridoma secreting monoclonal antibody highly specific to hemoglobin Bart's (γ4) |
title_short |
Production of a mouse hybridoma secreting monoclonal antibody highly specific to hemoglobin Bart's (γ4) |
title_full |
Production of a mouse hybridoma secreting monoclonal antibody highly specific to hemoglobin Bart's (γ4) |
title_fullStr |
Production of a mouse hybridoma secreting monoclonal antibody highly specific to hemoglobin Bart's (γ4) |
title_full_unstemmed |
Production of a mouse hybridoma secreting monoclonal antibody highly specific to hemoglobin Bart's (γ4) |
title_sort |
production of a mouse hybridoma secreting monoclonal antibody highly specific to hemoglobin bart's (γ4) |
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2018 |
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https://www.scopus.com/inward/record.uri?partnerID=HzOxMe3b&scp=37849185172&origin=inward http://cmuir.cmu.ac.th/jspui/handle/6653943832/61780 |
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