Mucosal mast cell responses in rats (Rattus norvegicus) experimentally infected with Centrocestus caninus

Mucosal mast cell (MMC) responses and worm recovery rates in rats experimentally infected with Centrocestus caninus were investigated. Metacercariae of C. caninus, procured from goldfish, Carassius auratus, were orally administered to twenty-five male rats (300 metacercariae each rat). The infected...

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Main Authors: Supap Saenphet, Chalobol Wongsawad, Kanokporn Saenphet, Jong Yil Chai
Format: Journal
Published: 2018
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http://cmuir.cmu.ac.th/jspui/handle/6653943832/61792
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Institution: Chiang Mai University
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spelling th-cmuir.6653943832-617922018-09-11T08:59:15Z Mucosal mast cell responses in rats (Rattus norvegicus) experimentally infected with Centrocestus caninus Supap Saenphet Chalobol Wongsawad Kanokporn Saenphet Jong Yil Chai Medicine Mucosal mast cell (MMC) responses and worm recovery rates in rats experimentally infected with Centrocestus caninus were investigated. Metacercariae of C. caninus, procured from goldfish, Carassius auratus, were orally administered to twenty-five male rats (300 metacercariae each rat). The infected rats were sacrificed on days 3, 7, 14, 21 and 28 post-infection (Pl) along with the control rats. Worm recovery was performed from each part of small intestine. To investigate MMC, duodenal, jejunal and ileal paraffinized-tissue sections were processed and stained with 1% alcian blue and 0.5% safranin-O. The average worm recovery rates were 42.8, 37.7, 21.2, 12.5 and 3.7% on days 3, 7, 14, 21 and 28 PI, respectively. The majority of the worms (98.9%) were collected from the duodenum and jejunum. The MMC numbers in the infected rats were significantly higher than those of the controls (p<0.05). A peak level was observed on days 14 PI and the numbers gradually decreased thereafter. The results reveal that MMC plays an important role in the expulsion of C. caninus from the host intestine. A more precise description of the role the MMC plays in helminth expulsion is still needed to understand the mechanism of host defense against intestinal helminthic infection, along with other effector cells, such as goblet cells. 2018-09-11T08:59:15Z 2018-09-11T08:59:15Z 2006-12-01 Journal 01251562 2-s2.0-33845792929 https://www.scopus.com/inward/record.uri?partnerID=HzOxMe3b&scp=33845792929&origin=inward http://cmuir.cmu.ac.th/jspui/handle/6653943832/61792
institution Chiang Mai University
building Chiang Mai University Library
country Thailand
collection CMU Intellectual Repository
topic Medicine
spellingShingle Medicine
Supap Saenphet
Chalobol Wongsawad
Kanokporn Saenphet
Jong Yil Chai
Mucosal mast cell responses in rats (Rattus norvegicus) experimentally infected with Centrocestus caninus
description Mucosal mast cell (MMC) responses and worm recovery rates in rats experimentally infected with Centrocestus caninus were investigated. Metacercariae of C. caninus, procured from goldfish, Carassius auratus, were orally administered to twenty-five male rats (300 metacercariae each rat). The infected rats were sacrificed on days 3, 7, 14, 21 and 28 post-infection (Pl) along with the control rats. Worm recovery was performed from each part of small intestine. To investigate MMC, duodenal, jejunal and ileal paraffinized-tissue sections were processed and stained with 1% alcian blue and 0.5% safranin-O. The average worm recovery rates were 42.8, 37.7, 21.2, 12.5 and 3.7% on days 3, 7, 14, 21 and 28 PI, respectively. The majority of the worms (98.9%) were collected from the duodenum and jejunum. The MMC numbers in the infected rats were significantly higher than those of the controls (p<0.05). A peak level was observed on days 14 PI and the numbers gradually decreased thereafter. The results reveal that MMC plays an important role in the expulsion of C. caninus from the host intestine. A more precise description of the role the MMC plays in helminth expulsion is still needed to understand the mechanism of host defense against intestinal helminthic infection, along with other effector cells, such as goblet cells.
format Journal
author Supap Saenphet
Chalobol Wongsawad
Kanokporn Saenphet
Jong Yil Chai
author_facet Supap Saenphet
Chalobol Wongsawad
Kanokporn Saenphet
Jong Yil Chai
author_sort Supap Saenphet
title Mucosal mast cell responses in rats (Rattus norvegicus) experimentally infected with Centrocestus caninus
title_short Mucosal mast cell responses in rats (Rattus norvegicus) experimentally infected with Centrocestus caninus
title_full Mucosal mast cell responses in rats (Rattus norvegicus) experimentally infected with Centrocestus caninus
title_fullStr Mucosal mast cell responses in rats (Rattus norvegicus) experimentally infected with Centrocestus caninus
title_full_unstemmed Mucosal mast cell responses in rats (Rattus norvegicus) experimentally infected with Centrocestus caninus
title_sort mucosal mast cell responses in rats (rattus norvegicus) experimentally infected with centrocestus caninus
publishDate 2018
url https://www.scopus.com/inward/record.uri?partnerID=HzOxMe3b&scp=33845792929&origin=inward
http://cmuir.cmu.ac.th/jspui/handle/6653943832/61792
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