Analysis of DNA corresponding to group A streptococcal A- and B-regions of M protein by restriction fragment length polymorphism

The emm gene amplicons encoding the N- and partial C-terminal regions of the M protein from Group A streptococcus (GAS) isolates were amplified by PCR and digested with a restriction endonuclease, AluI. Digested PCR products were then resolved by polyacrylamide gel electrophoresis. Using one pair of...

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Main Authors: N. Yoonim, C. Olive, C. Pruksacharkunakorn, M. F. Good, S. Pruksakorn
Format: Book Series
Published: 2018
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http://cmuir.cmu.ac.th/jspui/handle/6653943832/61880
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Institution: Chiang Mai University
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spelling th-cmuir.6653943832-618802018-09-11T09:00:41Z Analysis of DNA corresponding to group A streptococcal A- and B-regions of M protein by restriction fragment length polymorphism N. Yoonim C. Olive C. Pruksacharkunakorn M. F. Good S. Pruksakorn Medicine The emm gene amplicons encoding the N- and partial C-terminal regions of the M protein from Group A streptococcus (GAS) isolates were amplified by PCR and digested with a restriction endonuclease, AluI. Digested PCR products were then resolved by polyacrylamide gel electrophoresis. Using one pair of primers, 13 known GAS M types showed one to four bands of PCR products. Digested with AluI, they exhibited different restriction RFLP patterns. Of 106 GAS isolates examined from normal population and patients with GAS-associated complications, 95 isolates showed RFLP patterns that corresponded to the 13 known M types. The other 11 isolates demonstrated different RFLP patterns other than the known M types. They were then analyzed by DNA sequencing and other six M types were obtained. In addition, M93 GAS was the most common from the population studied, which is consistent with a previous study of Thai GAS isolates. This PCR-RFLP protocol has the potential for the rapid screening of GAS emm types, and would be able to characterize the number of C-region in different types of GAS. This method is considerably advantageous as an alternative M typing approach for epidemiological studies in developing countries in which GAS is endemic and DNA sequencer and budgets are limited. © 2005 Elsevier B.V. All rights reserved. 2018-09-11T09:00:41Z 2018-09-11T09:00:41Z 2006-04-01 Book Series 05315131 2-s2.0-33645951662 10.1016/j.ics.2005.11.006 https://www.scopus.com/inward/record.uri?partnerID=HzOxMe3b&scp=33645951662&origin=inward http://cmuir.cmu.ac.th/jspui/handle/6653943832/61880
institution Chiang Mai University
building Chiang Mai University Library
country Thailand
collection CMU Intellectual Repository
topic Medicine
spellingShingle Medicine
N. Yoonim
C. Olive
C. Pruksacharkunakorn
M. F. Good
S. Pruksakorn
Analysis of DNA corresponding to group A streptococcal A- and B-regions of M protein by restriction fragment length polymorphism
description The emm gene amplicons encoding the N- and partial C-terminal regions of the M protein from Group A streptococcus (GAS) isolates were amplified by PCR and digested with a restriction endonuclease, AluI. Digested PCR products were then resolved by polyacrylamide gel electrophoresis. Using one pair of primers, 13 known GAS M types showed one to four bands of PCR products. Digested with AluI, they exhibited different restriction RFLP patterns. Of 106 GAS isolates examined from normal population and patients with GAS-associated complications, 95 isolates showed RFLP patterns that corresponded to the 13 known M types. The other 11 isolates demonstrated different RFLP patterns other than the known M types. They were then analyzed by DNA sequencing and other six M types were obtained. In addition, M93 GAS was the most common from the population studied, which is consistent with a previous study of Thai GAS isolates. This PCR-RFLP protocol has the potential for the rapid screening of GAS emm types, and would be able to characterize the number of C-region in different types of GAS. This method is considerably advantageous as an alternative M typing approach for epidemiological studies in developing countries in which GAS is endemic and DNA sequencer and budgets are limited. © 2005 Elsevier B.V. All rights reserved.
format Book Series
author N. Yoonim
C. Olive
C. Pruksacharkunakorn
M. F. Good
S. Pruksakorn
author_facet N. Yoonim
C. Olive
C. Pruksacharkunakorn
M. F. Good
S. Pruksakorn
author_sort N. Yoonim
title Analysis of DNA corresponding to group A streptococcal A- and B-regions of M protein by restriction fragment length polymorphism
title_short Analysis of DNA corresponding to group A streptococcal A- and B-regions of M protein by restriction fragment length polymorphism
title_full Analysis of DNA corresponding to group A streptococcal A- and B-regions of M protein by restriction fragment length polymorphism
title_fullStr Analysis of DNA corresponding to group A streptococcal A- and B-regions of M protein by restriction fragment length polymorphism
title_full_unstemmed Analysis of DNA corresponding to group A streptococcal A- and B-regions of M protein by restriction fragment length polymorphism
title_sort analysis of dna corresponding to group a streptococcal a- and b-regions of m protein by restriction fragment length polymorphism
publishDate 2018
url https://www.scopus.com/inward/record.uri?partnerID=HzOxMe3b&scp=33645951662&origin=inward
http://cmuir.cmu.ac.th/jspui/handle/6653943832/61880
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