Ribosomal DNA fingerprinting in the identification of non sporulating endophytes from Magnolia liliifera (Magnoliaceae)

Ribosomal DNA fingerprinting in the identification of non sporulating endophytes from Magnolia liliifera (Magnoliaceae)

The sporulating endophytic fungi isolated from Magnolia liliifera were identified to genus or species level using morphological characters. Non-sporulating isolates are generally termed as 'mycelia sterilia' and are grouped as 'morphospecies' based on similar cultural characters....

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Main Authors: Itthayakorn Promputtha, Rajesh Jeewon, Saisamorn Lumyong, Eric H.C. McKenzie, Kevin D. Hyde
Format: Journal
Published: 2018
Subjects:
Agricultural and Biological Sciences
Environmental Science
Online Access:https://www.scopus.com/inward/record.uri?partnerID=HzOxMe3b&scp=30944436894&origin=inward
http://cmuir.cmu.ac.th/jspui/handle/6653943832/61999
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Institution: Chiang Mai University
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spelling th-cmuir.6653943832-619992018-09-11T09:23:56Z Ribosomal DNA fingerprinting in the identification of non sporulating endophytes from Magnolia liliifera (Magnoliaceae) Itthayakorn Promputtha Rajesh Jeewon Saisamorn Lumyong Eric H.C. McKenzie Kevin D. Hyde Agricultural and Biological Sciences Environmental Science The sporulating endophytic fungi isolated from Magnolia liliifera were identified to genus or species level using morphological characters. Non-sporulating isolates are generally termed as 'mycelia sterilia' and are grouped as 'morphospecies' based on similar cultural characters. Mycelia sterilia were grouped to 31 morphospecies and were further identified based on ribosomal DNA (rDNA) sequence analysis. The 5.8S gene, ITS1 and ITS2 regions of rDNA from all morphospecies were amplified and sequenced. Phylogenetic analysis indicated that MS88 were related to the genus Massarina (Lophiostomataceae), MS9, MS11 and MS47 to Xylaria (Xylariaceae), MS19 to Glomerella (Phyllachoraceae), MS25 to Hypoxylon (Xylariaceae), MS27 to Bionectria (Bionectriaceae) and the remaining 24 morphospecies were related to Phomopsis and Diaporthe (anamorphic Phomopsis). 2018-09-11T09:20:45Z 2018-09-11T09:20:45Z 2005-10-31 Journal 15602745 2-s2.0-30944436894 https://www.scopus.com/inward/record.uri?partnerID=HzOxMe3b&scp=30944436894&origin=inward http://cmuir.cmu.ac.th/jspui/handle/6653943832/61999
institution Chiang Mai University
building Chiang Mai University Library
country Thailand
collection CMU Intellectual Repository
topic Agricultural and Biological Sciences
Environmental Science
spellingShingle Agricultural and Biological Sciences
Environmental Science
Itthayakorn Promputtha
Rajesh Jeewon
Saisamorn Lumyong
Eric H.C. McKenzie
Kevin D. Hyde
Ribosomal DNA fingerprinting in the identification of non sporulating endophytes from Magnolia liliifera (Magnoliaceae)
description The sporulating endophytic fungi isolated from Magnolia liliifera were identified to genus or species level using morphological characters. Non-sporulating isolates are generally termed as 'mycelia sterilia' and are grouped as 'morphospecies' based on similar cultural characters. Mycelia sterilia were grouped to 31 morphospecies and were further identified based on ribosomal DNA (rDNA) sequence analysis. The 5.8S gene, ITS1 and ITS2 regions of rDNA from all morphospecies were amplified and sequenced. Phylogenetic analysis indicated that MS88 were related to the genus Massarina (Lophiostomataceae), MS9, MS11 and MS47 to Xylaria (Xylariaceae), MS19 to Glomerella (Phyllachoraceae), MS25 to Hypoxylon (Xylariaceae), MS27 to Bionectria (Bionectriaceae) and the remaining 24 morphospecies were related to Phomopsis and Diaporthe (anamorphic Phomopsis).
format Journal
author Itthayakorn Promputtha
Rajesh Jeewon
Saisamorn Lumyong
Eric H.C. McKenzie
Kevin D. Hyde
author_facet Itthayakorn Promputtha
Rajesh Jeewon
Saisamorn Lumyong
Eric H.C. McKenzie
Kevin D. Hyde
author_sort Itthayakorn Promputtha
title Ribosomal DNA fingerprinting in the identification of non sporulating endophytes from Magnolia liliifera (Magnoliaceae)
title_short Ribosomal DNA fingerprinting in the identification of non sporulating endophytes from Magnolia liliifera (Magnoliaceae)
title_full Ribosomal DNA fingerprinting in the identification of non sporulating endophytes from Magnolia liliifera (Magnoliaceae)
title_fullStr Ribosomal DNA fingerprinting in the identification of non sporulating endophytes from Magnolia liliifera (Magnoliaceae)
title_full_unstemmed Ribosomal DNA fingerprinting in the identification of non sporulating endophytes from Magnolia liliifera (Magnoliaceae)
title_sort ribosomal dna fingerprinting in the identification of non sporulating endophytes from magnolia liliifera (magnoliaceae)
publishDate 2018
url https://www.scopus.com/inward/record.uri?partnerID=HzOxMe3b&scp=30944436894&origin=inward
http://cmuir.cmu.ac.th/jspui/handle/6653943832/61999
_version_ 1681425725342613504

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