Novel approach for mono-segmented flow micro-titration with sequential injection using a lab-on-valve system: A model study for the assay of acidity in fruit juices

A new concept for micro-titration using a "lab-on-valve" (LOV) system with sequential injection of mono-segmented flow is proposed. The performance of the system was demonstrated by the assay of acidity in fruit juices which is based on acid-base neutralization. A standard/sample solution...

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Main Authors: Jaroon Jakmunee, Lop Pathimapornlert, Supaporn Kradtap Hartwell, Kate Grudpan
Format: Journal
Published: 2018
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http://cmuir.cmu.ac.th/jspui/handle/6653943832/62111
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Institution: Chiang Mai University
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spelling th-cmuir.6653943832-621112018-09-11T09:24:00Z Novel approach for mono-segmented flow micro-titration with sequential injection using a lab-on-valve system: A model study for the assay of acidity in fruit juices Jaroon Jakmunee Lop Pathimapornlert Supaporn Kradtap Hartwell Kate Grudpan Biochemistry, Genetics and Molecular Biology Chemistry Environmental Science A new concept for micro-titration using a "lab-on-valve" (LOV) system with sequential injection of mono-segmented flow is proposed. The performance of the system was demonstrated by the assay of acidity in fruit juices which is based on acid-base neutralization. A standard/sample solution containing citric acid, indicator, sodium hydroxide, were sandwiched between air segments and were aspirated in microliter volumes through a selection valve into a holding coil. The acid, indicator, and base were mixed by flow reversal. After removing air segments, the solution was pushed to the detector for monitoring of the change in absorbance of the indicator color, which depended on the concentration of the remaining base. With LOV, microliter volumes of the solution can be detected without dispersion of the color zone. A calibration graph (plot of absorbance vs. acidity value) in the range of 0.2-1.2% (w/v) as citric acid was established. Sample throughput of 30 sample h-1 and good reproducibility (RSD = 1.2%, n = 11 for 0.6% acidity) were achieved. The procedure has been applied to determine acidity in fruit juices. © The Royal Society of Chemistry 2005. 2018-09-11T09:22:02Z 2018-09-11T09:22:02Z 2005-03-01 Journal 00032654 2-s2.0-15244357839 10.1039/b413585g https://www.scopus.com/inward/record.uri?partnerID=HzOxMe3b&scp=15244357839&origin=inward http://cmuir.cmu.ac.th/jspui/handle/6653943832/62111
institution Chiang Mai University
building Chiang Mai University Library
country Thailand
collection CMU Intellectual Repository
topic Biochemistry, Genetics and Molecular Biology
Chemistry
Environmental Science
spellingShingle Biochemistry, Genetics and Molecular Biology
Chemistry
Environmental Science
Jaroon Jakmunee
Lop Pathimapornlert
Supaporn Kradtap Hartwell
Kate Grudpan
Novel approach for mono-segmented flow micro-titration with sequential injection using a lab-on-valve system: A model study for the assay of acidity in fruit juices
description A new concept for micro-titration using a "lab-on-valve" (LOV) system with sequential injection of mono-segmented flow is proposed. The performance of the system was demonstrated by the assay of acidity in fruit juices which is based on acid-base neutralization. A standard/sample solution containing citric acid, indicator, sodium hydroxide, were sandwiched between air segments and were aspirated in microliter volumes through a selection valve into a holding coil. The acid, indicator, and base were mixed by flow reversal. After removing air segments, the solution was pushed to the detector for monitoring of the change in absorbance of the indicator color, which depended on the concentration of the remaining base. With LOV, microliter volumes of the solution can be detected without dispersion of the color zone. A calibration graph (plot of absorbance vs. acidity value) in the range of 0.2-1.2% (w/v) as citric acid was established. Sample throughput of 30 sample h-1 and good reproducibility (RSD = 1.2%, n = 11 for 0.6% acidity) were achieved. The procedure has been applied to determine acidity in fruit juices. © The Royal Society of Chemistry 2005.
format Journal
author Jaroon Jakmunee
Lop Pathimapornlert
Supaporn Kradtap Hartwell
Kate Grudpan
author_facet Jaroon Jakmunee
Lop Pathimapornlert
Supaporn Kradtap Hartwell
Kate Grudpan
author_sort Jaroon Jakmunee
title Novel approach for mono-segmented flow micro-titration with sequential injection using a lab-on-valve system: A model study for the assay of acidity in fruit juices
title_short Novel approach for mono-segmented flow micro-titration with sequential injection using a lab-on-valve system: A model study for the assay of acidity in fruit juices
title_full Novel approach for mono-segmented flow micro-titration with sequential injection using a lab-on-valve system: A model study for the assay of acidity in fruit juices
title_fullStr Novel approach for mono-segmented flow micro-titration with sequential injection using a lab-on-valve system: A model study for the assay of acidity in fruit juices
title_full_unstemmed Novel approach for mono-segmented flow micro-titration with sequential injection using a lab-on-valve system: A model study for the assay of acidity in fruit juices
title_sort novel approach for mono-segmented flow micro-titration with sequential injection using a lab-on-valve system: a model study for the assay of acidity in fruit juices
publishDate 2018
url https://www.scopus.com/inward/record.uri?partnerID=HzOxMe3b&scp=15244357839&origin=inward
http://cmuir.cmu.ac.th/jspui/handle/6653943832/62111
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