Different techniques for urinary protein analysis of normal and lung cancer patients
Many components in urine are useful in clinical diagnosis and urinary proteins are known as important components to define many diseases such as proteinuria, kidney, bladder and urinary tract diseases. In this study, we focused on the comparison of different sample preparation methods for isolating...
Saved in:
Main Authors: | , , , , |
---|---|
Format: | Journal |
Published: |
2018
|
Subjects: | |
Online Access: | https://www.scopus.com/inward/record.uri?partnerID=HzOxMe3b&scp=16344387536&origin=inward http://cmuir.cmu.ac.th/jspui/handle/6653943832/62112 |
Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
Institution: | Chiang Mai University |
id |
th-cmuir.6653943832-62112 |
---|---|
record_format |
dspace |
spelling |
th-cmuir.6653943832-621122018-09-11T09:22:03Z Different techniques for urinary protein analysis of normal and lung cancer patients Payungsak Tantipaiboonwong Supachok Sinchaikul Supawadee Sriyam Suree Phutrakul Shui Tein Chen Biochemistry, Genetics and Molecular Biology Many components in urine are useful in clinical diagnosis and urinary proteins are known as important components to define many diseases such as proteinuria, kidney, bladder and urinary tract diseases. In this study, we focused on the comparison of different sample preparation methods for isolating urinary proteins prior to protein analysis of pooled healthy and lung cancer patient samples. Selective method was used for preliminary investigation of some putative urinary protein markers. Urine samples were passed first through a gel filtration column (PD-10 desalting column) to remove high salts and subsequently concentrated. Remaining interferences were removed by ultrafiltration or four precipitation methods. The analysis of urinary proteins by high-performance liquid chromatography and sodium dodecyl sulfate-polyacrylamide gel electrophoresis showed many similarities in profiles among preparation methods and a few profiles were different between normal and lung cancer patients. In contrast, the results of two-dimensional gel electrophoresis (2-DE) showed more distinctly different protein patterns. Our finding showed that the sequential preparation of urinary proteins by gel filtration and ultrafiltration could retain most urinary proteins which demonstrated the highest protein spots on 2-D gels and able to identify preliminary urinary protein markers related to cancer. Although sequential preparation of urine samples by gel filtration and protein precipitation resulted in low amounts of proteins on 2-D gels, high Mr proteins were easily detected. Therefore, there are alternative choices for urine sample preparation for studying the urinary proteome and identifing urinary protein markers important for further preclinical diagnostic and therapeutic applications. © 2005 WILEY-VCH Verlag GmbH & Co. KGaA. 2018-09-11T09:22:03Z 2018-09-11T09:22:03Z 2005-03-01 Journal 16159853 2-s2.0-16344387536 10.1002/pmic.200401143 https://www.scopus.com/inward/record.uri?partnerID=HzOxMe3b&scp=16344387536&origin=inward http://cmuir.cmu.ac.th/jspui/handle/6653943832/62112 |
institution |
Chiang Mai University |
building |
Chiang Mai University Library |
country |
Thailand |
collection |
CMU Intellectual Repository |
topic |
Biochemistry, Genetics and Molecular Biology |
spellingShingle |
Biochemistry, Genetics and Molecular Biology Payungsak Tantipaiboonwong Supachok Sinchaikul Supawadee Sriyam Suree Phutrakul Shui Tein Chen Different techniques for urinary protein analysis of normal and lung cancer patients |
description |
Many components in urine are useful in clinical diagnosis and urinary proteins are known as important components to define many diseases such as proteinuria, kidney, bladder and urinary tract diseases. In this study, we focused on the comparison of different sample preparation methods for isolating urinary proteins prior to protein analysis of pooled healthy and lung cancer patient samples. Selective method was used for preliminary investigation of some putative urinary protein markers. Urine samples were passed first through a gel filtration column (PD-10 desalting column) to remove high salts and subsequently concentrated. Remaining interferences were removed by ultrafiltration or four precipitation methods. The analysis of urinary proteins by high-performance liquid chromatography and sodium dodecyl sulfate-polyacrylamide gel electrophoresis showed many similarities in profiles among preparation methods and a few profiles were different between normal and lung cancer patients. In contrast, the results of two-dimensional gel electrophoresis (2-DE) showed more distinctly different protein patterns. Our finding showed that the sequential preparation of urinary proteins by gel filtration and ultrafiltration could retain most urinary proteins which demonstrated the highest protein spots on 2-D gels and able to identify preliminary urinary protein markers related to cancer. Although sequential preparation of urine samples by gel filtration and protein precipitation resulted in low amounts of proteins on 2-D gels, high Mr proteins were easily detected. Therefore, there are alternative choices for urine sample preparation for studying the urinary proteome and identifing urinary protein markers important for further preclinical diagnostic and therapeutic applications. © 2005 WILEY-VCH Verlag GmbH & Co. KGaA. |
format |
Journal |
author |
Payungsak Tantipaiboonwong Supachok Sinchaikul Supawadee Sriyam Suree Phutrakul Shui Tein Chen |
author_facet |
Payungsak Tantipaiboonwong Supachok Sinchaikul Supawadee Sriyam Suree Phutrakul Shui Tein Chen |
author_sort |
Payungsak Tantipaiboonwong |
title |
Different techniques for urinary protein analysis of normal and lung cancer patients |
title_short |
Different techniques for urinary protein analysis of normal and lung cancer patients |
title_full |
Different techniques for urinary protein analysis of normal and lung cancer patients |
title_fullStr |
Different techniques for urinary protein analysis of normal and lung cancer patients |
title_full_unstemmed |
Different techniques for urinary protein analysis of normal and lung cancer patients |
title_sort |
different techniques for urinary protein analysis of normal and lung cancer patients |
publishDate |
2018 |
url |
https://www.scopus.com/inward/record.uri?partnerID=HzOxMe3b&scp=16344387536&origin=inward http://cmuir.cmu.ac.th/jspui/handle/6653943832/62112 |
_version_ |
1681425746305744896 |