Theoretical analysis on ratiometric fluorescent indicators caused biased estimates of intracellular free calcium concentrations

• Main Authors: Zhiwen Hu, Liangdeng Yu, Zengliang Yu
• Format: Journal
• Published: 2018
• Subjects: Biochemistry, Genetics and Molecular Biology; Health Professions; Medicine; Physics and Astronomy
• Online Access: https://www.scopus.com/inward/record.uri?partnerID=HzOxMe3b&scp=13544260798&origin=inward; http://cmuir.cmu.ac.th/jspui/handle/6653943832/62113
• Institution: Chiang Mai University

Ratiometric fluorescent calcium indicator dyes have been widely used for the study of the role of Ca2+in cell physiopathology. Although these ratiometric dyes offer several advantages over others, they suffer some drawbacks which cause serious errors in measurement of intracellular Ca2+concentration, [Ca2+i. The present study systematically analyzes theoretical reasons and technical sources of discrepancies occurring in the measurement of the characteristics of the agonists-induced cells [Ca2+i. In order to avoid the errors and achieve the accurate determination of [Ca2+]i, this study proposes solutions and suggests some critical measures in both theoretical and technical aspects. Therefore, this analysis can be a valuable tool in clarifying proper usages of fluorescent dyes for [Ca2+imeasurements. © 2004 Elsevier B.V. All rights reserved.