Detection of cytomegalovirus in vitreous, aqueous and conjunctiva by Polymerase Chain Reaction (PCR)

Objective: To evaluate the diagnostic value of polymerase chain reaction (PCR) performed on vitreous, aqueous and conjunctiva for the detection of cytomegalovirus in AIDS patients with a clinical diagnosis of cytomegalovirus retinitis. Material and Method: PCR-based assay was used to detect cytomega...

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Main Authors: Kessara Pathanapitoon, Somsaguan Ausayakhun, Paradee Kunavisarut, Arporn Pungrasame, Wasna Sirirungsi
Format: Journal
Published: 2018
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http://cmuir.cmu.ac.th/jspui/handle/6653943832/62420
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Institution: Chiang Mai University
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spelling th-cmuir.6653943832-624202018-09-11T09:27:07Z Detection of cytomegalovirus in vitreous, aqueous and conjunctiva by Polymerase Chain Reaction (PCR) Kessara Pathanapitoon Somsaguan Ausayakhun Paradee Kunavisarut Arporn Pungrasame Wasna Sirirungsi Medicine Objective: To evaluate the diagnostic value of polymerase chain reaction (PCR) performed on vitreous, aqueous and conjunctiva for the detection of cytomegalovirus in AIDS patients with a clinical diagnosis of cytomegalovirus retinitis. Material and Method: PCR-based assay was used to detect cytomegalovirus DNA in vitreous, aqueous and conjunctival samples from 24 patients with the acquired immunodeficiency syndrome (AIDS) who had untreated clinically diagnosed cytomegalovirus retinitis and from 15 immunocompetent patients, including 11 with retinal detachment, 2 with macular hole and 2 with vitreous hemorrhage. Results: Cytomegalovirus DNA was detected in 16, 9 and 3 of 24 vitreous, aqueous and conjunctival samples, respectively, from patients with AIDS, untreated clinically diagnosis of cytomegalovirus retinitis; and in one patient out of 15 vitreous, aqueous and conjunctival samples from immunocompetent patients with vitreoretinal diseases. Conclusion: The use of PCR in the detection of cytomegalovirus in vitreous, aqueous and conjunctival samples had an equal specificity of 93% and had sensitivity of 67, 37 and 12%, respectively. 2018-09-11T09:27:07Z 2018-09-11T09:27:07Z 2005-02-01 Journal 01252208 2-s2.0-20444464029 https://www.scopus.com/inward/record.uri?partnerID=HzOxMe3b&scp=20444464029&origin=inward http://cmuir.cmu.ac.th/jspui/handle/6653943832/62420
institution Chiang Mai University
building Chiang Mai University Library
country Thailand
collection CMU Intellectual Repository
topic Medicine
spellingShingle Medicine
Kessara Pathanapitoon
Somsaguan Ausayakhun
Paradee Kunavisarut
Arporn Pungrasame
Wasna Sirirungsi
Detection of cytomegalovirus in vitreous, aqueous and conjunctiva by Polymerase Chain Reaction (PCR)
description Objective: To evaluate the diagnostic value of polymerase chain reaction (PCR) performed on vitreous, aqueous and conjunctiva for the detection of cytomegalovirus in AIDS patients with a clinical diagnosis of cytomegalovirus retinitis. Material and Method: PCR-based assay was used to detect cytomegalovirus DNA in vitreous, aqueous and conjunctival samples from 24 patients with the acquired immunodeficiency syndrome (AIDS) who had untreated clinically diagnosed cytomegalovirus retinitis and from 15 immunocompetent patients, including 11 with retinal detachment, 2 with macular hole and 2 with vitreous hemorrhage. Results: Cytomegalovirus DNA was detected in 16, 9 and 3 of 24 vitreous, aqueous and conjunctival samples, respectively, from patients with AIDS, untreated clinically diagnosis of cytomegalovirus retinitis; and in one patient out of 15 vitreous, aqueous and conjunctival samples from immunocompetent patients with vitreoretinal diseases. Conclusion: The use of PCR in the detection of cytomegalovirus in vitreous, aqueous and conjunctival samples had an equal specificity of 93% and had sensitivity of 67, 37 and 12%, respectively.
format Journal
author Kessara Pathanapitoon
Somsaguan Ausayakhun
Paradee Kunavisarut
Arporn Pungrasame
Wasna Sirirungsi
author_facet Kessara Pathanapitoon
Somsaguan Ausayakhun
Paradee Kunavisarut
Arporn Pungrasame
Wasna Sirirungsi
author_sort Kessara Pathanapitoon
title Detection of cytomegalovirus in vitreous, aqueous and conjunctiva by Polymerase Chain Reaction (PCR)
title_short Detection of cytomegalovirus in vitreous, aqueous and conjunctiva by Polymerase Chain Reaction (PCR)
title_full Detection of cytomegalovirus in vitreous, aqueous and conjunctiva by Polymerase Chain Reaction (PCR)
title_fullStr Detection of cytomegalovirus in vitreous, aqueous and conjunctiva by Polymerase Chain Reaction (PCR)
title_full_unstemmed Detection of cytomegalovirus in vitreous, aqueous and conjunctiva by Polymerase Chain Reaction (PCR)
title_sort detection of cytomegalovirus in vitreous, aqueous and conjunctiva by polymerase chain reaction (pcr)
publishDate 2018
url https://www.scopus.com/inward/record.uri?partnerID=HzOxMe3b&scp=20444464029&origin=inward
http://cmuir.cmu.ac.th/jspui/handle/6653943832/62420
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