Molecular epidemiology of classic, MLB and VA astroviruses isolated from <5 year-old children with gastroenteritis in Thailand, 2011–2016

© 2018 Elsevier B.V. This study aimed to investigate the molecular epidemiology and genetic diversity of astrovirus circulating in pediatric patients with acute gastroenteritis in Thailand. A total of 2034 fecal specimens collected between 2011 and 2016 from children hospitalized with acute gastroen...

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Main Authors: Kattareeya Kumthip, Pattara Khamrin, Hiroshi Ushijima, Niwat Maneekarn
Format: Journal
Published: 2018
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http://cmuir.cmu.ac.th/jspui/handle/6653943832/62490
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Institution: Chiang Mai University
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spelling th-cmuir.6653943832-624902018-11-29T07:50:01Z Molecular epidemiology of classic, MLB and VA astroviruses isolated from <5 year-old children with gastroenteritis in Thailand, 2011–2016 Kattareeya Kumthip Pattara Khamrin Hiroshi Ushijima Niwat Maneekarn Agricultural and Biological Sciences Biochemistry, Genetics and Molecular Biology Immunology and Microbiology Medicine © 2018 Elsevier B.V. This study aimed to investigate the molecular epidemiology and genetic diversity of astrovirus circulating in pediatric patients with acute gastroenteritis in Thailand. A total of 2034 fecal specimens collected between 2011 and 2016 from children hospitalized with acute gastroenteritis were analyzed for astrovirus infection using RT-PCR and nucleotide sequencing techniques. Overall, 54 samples were positive for astrovirus (2.6%). About half of the virus-positive samples (57.4%) were co-infection cases, mainly with rotavirus or norovirus. Genotyping revealed that three major clades, classic human astrovirus (HAstV) and newly emerging MLB and VA astroviruses co-circulated in the study population. At least seven different genotypes were identified and HAstV1 was the most predominant genotype detected (50%), followed by MLB1 (16.7%), HAstV4 (11.1%), HAstV5 and HAstV8 (7.4% each), MLB2 and VA2 (3.7% each). This study provided a useful information and contributed to molecular epidemiology of astroviruses including the novel MLB and VA astoviruses have been characterized and reported for the first time in Thailand. 2018-11-29T07:29:24Z 2018-11-29T07:29:24Z 2018-11-01 Journal 15677257 15671348 2-s2.0-85052480981 10.1016/j.meegid.2018.08.024 https://www.scopus.com/inward/record.uri?partnerID=HzOxMe3b&scp=85052480981&origin=inward http://cmuir.cmu.ac.th/jspui/handle/6653943832/62490
institution Chiang Mai University
building Chiang Mai University Library
country Thailand
collection CMU Intellectual Repository
topic Agricultural and Biological Sciences
Biochemistry, Genetics and Molecular Biology
Immunology and Microbiology
Medicine
spellingShingle Agricultural and Biological Sciences
Biochemistry, Genetics and Molecular Biology
Immunology and Microbiology
Medicine
Kattareeya Kumthip
Pattara Khamrin
Hiroshi Ushijima
Niwat Maneekarn
Molecular epidemiology of classic, MLB and VA astroviruses isolated from <5 year-old children with gastroenteritis in Thailand, 2011–2016
description © 2018 Elsevier B.V. This study aimed to investigate the molecular epidemiology and genetic diversity of astrovirus circulating in pediatric patients with acute gastroenteritis in Thailand. A total of 2034 fecal specimens collected between 2011 and 2016 from children hospitalized with acute gastroenteritis were analyzed for astrovirus infection using RT-PCR and nucleotide sequencing techniques. Overall, 54 samples were positive for astrovirus (2.6%). About half of the virus-positive samples (57.4%) were co-infection cases, mainly with rotavirus or norovirus. Genotyping revealed that three major clades, classic human astrovirus (HAstV) and newly emerging MLB and VA astroviruses co-circulated in the study population. At least seven different genotypes were identified and HAstV1 was the most predominant genotype detected (50%), followed by MLB1 (16.7%), HAstV4 (11.1%), HAstV5 and HAstV8 (7.4% each), MLB2 and VA2 (3.7% each). This study provided a useful information and contributed to molecular epidemiology of astroviruses including the novel MLB and VA astoviruses have been characterized and reported for the first time in Thailand.
format Journal
author Kattareeya Kumthip
Pattara Khamrin
Hiroshi Ushijima
Niwat Maneekarn
author_facet Kattareeya Kumthip
Pattara Khamrin
Hiroshi Ushijima
Niwat Maneekarn
author_sort Kattareeya Kumthip
title Molecular epidemiology of classic, MLB and VA astroviruses isolated from <5 year-old children with gastroenteritis in Thailand, 2011–2016
title_short Molecular epidemiology of classic, MLB and VA astroviruses isolated from <5 year-old children with gastroenteritis in Thailand, 2011–2016
title_full Molecular epidemiology of classic, MLB and VA astroviruses isolated from <5 year-old children with gastroenteritis in Thailand, 2011–2016
title_fullStr Molecular epidemiology of classic, MLB and VA astroviruses isolated from <5 year-old children with gastroenteritis in Thailand, 2011–2016
title_full_unstemmed Molecular epidemiology of classic, MLB and VA astroviruses isolated from <5 year-old children with gastroenteritis in Thailand, 2011–2016
title_sort molecular epidemiology of classic, mlb and va astroviruses isolated from <5 year-old children with gastroenteritis in thailand, 2011–2016
publishDate 2018
url https://www.scopus.com/inward/record.uri?partnerID=HzOxMe3b&scp=85052480981&origin=inward
http://cmuir.cmu.ac.th/jspui/handle/6653943832/62490
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