Isolation and screening of aflatoxin-detoxifying yeast and bacteria from ruminal fluids to reduce aflatoxin B<inf>1</inf> contamination in dairy cattle feed

© 2018 The Society for Applied Microbiology Aims: To obtain yeast and bacteria from ruminal fluids that possess aflatoxin B1 (AFB1) detoxifying ability for use in animal feed. Methods and Results: Sources of isolation were ruminal fluids of three nonfistulated dairy cows, fed diets containing cassav...

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Main Authors: M. Intanoo, M. B. Kongkeitkajorn, V. Pattarajinda, J. K. Bernard, T. R. Callaway, W. Suriyasathaporn, Y. Phasuk
Format: Journal
Published: 2018
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http://cmuir.cmu.ac.th/jspui/handle/6653943832/62553
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Institution: Chiang Mai University
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spelling th-cmuir.6653943832-625532018-11-29T07:44:47Z Isolation and screening of aflatoxin-detoxifying yeast and bacteria from ruminal fluids to reduce aflatoxin B<inf>1</inf> contamination in dairy cattle feed M. Intanoo M. B. Kongkeitkajorn V. Pattarajinda J. K. Bernard T. R. Callaway W. Suriyasathaporn Y. Phasuk Biochemistry, Genetics and Molecular Biology Immunology and Microbiology © 2018 The Society for Applied Microbiology Aims: To obtain yeast and bacteria from ruminal fluids that possess aflatoxin B1 (AFB1) detoxifying ability for use in animal feed. Methods and Results: Sources of isolation were ruminal fluids of three nonfistulated dairy cows, fed diets containing cassava pulp, rice straw or distillery yeast sludge. The isolation was carried out to screen for the isolates that were active in both anaerobic and aerobic conditions. Three yeast and three bacteria isolates were selected. Up to 85% of AFB1 was detoxified by yeast isolates and up to 60% AFB1 reduction was evident by bacteria isolates. Two yeast isolates were identified as Kluyveromyces marxianus and one isolate as Pichia kudriavzevii. The three bacteria isolates were identified as Enterococcus faecium, Corynebacterium phoceense and C. vitaeruminis. All strains showed high biomass production when cultivated in medium with 80 g l−1 glucose. Conclusions: The isolated yeast and bacteria with AFB1 detoxifying ability showed a good potential to be applied as an aflatoxin-detoxifying agent to ingredients used to feed dairy cattle. Significance and Impact of the Study: The abilities of isolates to survive and be active in anaerobic and aerobic conditions rendered them to be active in cattle's rumen. Their biomass could be produced in bulk and used as feed supplement for aflatoxin detoxification in dairy cattle. 2018-11-29T07:32:13Z 2018-11-29T07:32:13Z 2018-12-01 Journal 13652672 13645072 2-s2.0-85052937414 10.1111/jam.14060 https://www.scopus.com/inward/record.uri?partnerID=HzOxMe3b&scp=85052937414&origin=inward http://cmuir.cmu.ac.th/jspui/handle/6653943832/62553
institution Chiang Mai University
building Chiang Mai University Library
country Thailand
collection CMU Intellectual Repository
topic Biochemistry, Genetics and Molecular Biology
Immunology and Microbiology
spellingShingle Biochemistry, Genetics and Molecular Biology
Immunology and Microbiology
M. Intanoo
M. B. Kongkeitkajorn
V. Pattarajinda
J. K. Bernard
T. R. Callaway
W. Suriyasathaporn
Y. Phasuk
Isolation and screening of aflatoxin-detoxifying yeast and bacteria from ruminal fluids to reduce aflatoxin B<inf>1</inf> contamination in dairy cattle feed
description © 2018 The Society for Applied Microbiology Aims: To obtain yeast and bacteria from ruminal fluids that possess aflatoxin B1 (AFB1) detoxifying ability for use in animal feed. Methods and Results: Sources of isolation were ruminal fluids of three nonfistulated dairy cows, fed diets containing cassava pulp, rice straw or distillery yeast sludge. The isolation was carried out to screen for the isolates that were active in both anaerobic and aerobic conditions. Three yeast and three bacteria isolates were selected. Up to 85% of AFB1 was detoxified by yeast isolates and up to 60% AFB1 reduction was evident by bacteria isolates. Two yeast isolates were identified as Kluyveromyces marxianus and one isolate as Pichia kudriavzevii. The three bacteria isolates were identified as Enterococcus faecium, Corynebacterium phoceense and C. vitaeruminis. All strains showed high biomass production when cultivated in medium with 80 g l−1 glucose. Conclusions: The isolated yeast and bacteria with AFB1 detoxifying ability showed a good potential to be applied as an aflatoxin-detoxifying agent to ingredients used to feed dairy cattle. Significance and Impact of the Study: The abilities of isolates to survive and be active in anaerobic and aerobic conditions rendered them to be active in cattle's rumen. Their biomass could be produced in bulk and used as feed supplement for aflatoxin detoxification in dairy cattle.
format Journal
author M. Intanoo
M. B. Kongkeitkajorn
V. Pattarajinda
J. K. Bernard
T. R. Callaway
W. Suriyasathaporn
Y. Phasuk
author_facet M. Intanoo
M. B. Kongkeitkajorn
V. Pattarajinda
J. K. Bernard
T. R. Callaway
W. Suriyasathaporn
Y. Phasuk
author_sort M. Intanoo
title Isolation and screening of aflatoxin-detoxifying yeast and bacteria from ruminal fluids to reduce aflatoxin B<inf>1</inf> contamination in dairy cattle feed
title_short Isolation and screening of aflatoxin-detoxifying yeast and bacteria from ruminal fluids to reduce aflatoxin B<inf>1</inf> contamination in dairy cattle feed
title_full Isolation and screening of aflatoxin-detoxifying yeast and bacteria from ruminal fluids to reduce aflatoxin B<inf>1</inf> contamination in dairy cattle feed
title_fullStr Isolation and screening of aflatoxin-detoxifying yeast and bacteria from ruminal fluids to reduce aflatoxin B<inf>1</inf> contamination in dairy cattle feed
title_full_unstemmed Isolation and screening of aflatoxin-detoxifying yeast and bacteria from ruminal fluids to reduce aflatoxin B<inf>1</inf> contamination in dairy cattle feed
title_sort isolation and screening of aflatoxin-detoxifying yeast and bacteria from ruminal fluids to reduce aflatoxin b<inf>1</inf> contamination in dairy cattle feed
publishDate 2018
url https://www.scopus.com/inward/record.uri?partnerID=HzOxMe3b&scp=85052937414&origin=inward
http://cmuir.cmu.ac.th/jspui/handle/6653943832/62553
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