Amniotic fluid: Source of valuable mesenchymal stem cells and alternatively used as cryopreserved solution

© 2018 Elsevier GmbH Mesenchymal stem cells (MSCs), which possess remarkable capabilities, are found in amniotic fluid (AF). The findings of several studies have shown the potential benefits of these cells in applications of regenerative medicine. In clinical applications, an over-period of time is...

Full description

Saved in:
Bibliographic Details
Main Authors: Suteera Narakornsak, Sirinda Aungsuchawan, Peraphan Pothacharoen, Chaniporn Puaninta, Runchana Markmee, Waleephan Tancharoen, Tanongsak Laowanitwattana, Naree Poovachiranon, Chawapon Thaojamnong
Format: Journal
Published: 2018
Subjects:
Online Access:https://www.scopus.com/inward/record.uri?partnerID=HzOxMe3b&scp=85055911810&origin=inward
http://cmuir.cmu.ac.th/jspui/handle/6653943832/62592
Tags: Add Tag
No Tags, Be the first to tag this record!
Institution: Chiang Mai University
id th-cmuir.6653943832-62592
record_format dspace
spelling th-cmuir.6653943832-625922018-11-29T07:53:55Z Amniotic fluid: Source of valuable mesenchymal stem cells and alternatively used as cryopreserved solution Suteera Narakornsak Sirinda Aungsuchawan Peraphan Pothacharoen Chaniporn Puaninta Runchana Markmee Waleephan Tancharoen Tanongsak Laowanitwattana Naree Poovachiranon Chawapon Thaojamnong Biochemistry, Genetics and Molecular Biology Medicine © 2018 Elsevier GmbH Mesenchymal stem cells (MSCs), which possess remarkable capabilities, are found in amniotic fluid (AF). The findings of several studies have shown the potential benefits of these cells in applications of regenerative medicine. In clinical applications, an over-period of time is required in a preparation process that makes cell collection become more necessary. Herein, the aim of this study was to preserve and characterize the cell's properties after cell cryopreservation into an appropriate cryogenic medium. The results illustrated that the highest hAF-MSCs viability was found when the cells were conserved in a solution of 5% DMSO + 10% FBS in AF. However, no statistical differences were identified in a chromosomal aberration of the post-thawed cells when compared to the non-frozen cells. These cells could also maintain their MSC features through the ability to express cell prolific quality, illustrating the typical MSC markers and immune privilege properties of CD44, CD73, CD90 and HLA-ABC. Additionally, post-thawed cells were able to differentiate into chondrogenic lineage by exhibiting chondrogenic related genes (SOX9, AGC, COL2A1) and proteins (transcription factor SOX9 protein (SOX9), cartilage oligomeric matrix protein (COMP) and aggrecan core protein (AGC)), as well as to present sGAGs accumulation. Interestingly, the use of a transmission electron microscope (TEM) uncovered the enrichment of the rough endoplasmic reticulum (rER) that coincided with euchromatin and the prominent nucleolus in the chondrogenic-induced cells that are normally found in the cells of natural cartilage. All in all, this study manifested that AF can be a major consideration and applied for use as a co-mixture of cryogenic medium. 2018-11-29T07:34:20Z 2018-11-29T07:34:20Z 2018-01-01 Journal 16180372 00651281 2-s2.0-85055911810 10.1016/j.acthis.2018.10.009 https://www.scopus.com/inward/record.uri?partnerID=HzOxMe3b&scp=85055911810&origin=inward http://cmuir.cmu.ac.th/jspui/handle/6653943832/62592
institution Chiang Mai University
building Chiang Mai University Library
country Thailand
collection CMU Intellectual Repository
topic Biochemistry, Genetics and Molecular Biology
Medicine
spellingShingle Biochemistry, Genetics and Molecular Biology
Medicine
Suteera Narakornsak
Sirinda Aungsuchawan
Peraphan Pothacharoen
Chaniporn Puaninta
Runchana Markmee
Waleephan Tancharoen
Tanongsak Laowanitwattana
Naree Poovachiranon
Chawapon Thaojamnong
Amniotic fluid: Source of valuable mesenchymal stem cells and alternatively used as cryopreserved solution
description © 2018 Elsevier GmbH Mesenchymal stem cells (MSCs), which possess remarkable capabilities, are found in amniotic fluid (AF). The findings of several studies have shown the potential benefits of these cells in applications of regenerative medicine. In clinical applications, an over-period of time is required in a preparation process that makes cell collection become more necessary. Herein, the aim of this study was to preserve and characterize the cell's properties after cell cryopreservation into an appropriate cryogenic medium. The results illustrated that the highest hAF-MSCs viability was found when the cells were conserved in a solution of 5% DMSO + 10% FBS in AF. However, no statistical differences were identified in a chromosomal aberration of the post-thawed cells when compared to the non-frozen cells. These cells could also maintain their MSC features through the ability to express cell prolific quality, illustrating the typical MSC markers and immune privilege properties of CD44, CD73, CD90 and HLA-ABC. Additionally, post-thawed cells were able to differentiate into chondrogenic lineage by exhibiting chondrogenic related genes (SOX9, AGC, COL2A1) and proteins (transcription factor SOX9 protein (SOX9), cartilage oligomeric matrix protein (COMP) and aggrecan core protein (AGC)), as well as to present sGAGs accumulation. Interestingly, the use of a transmission electron microscope (TEM) uncovered the enrichment of the rough endoplasmic reticulum (rER) that coincided with euchromatin and the prominent nucleolus in the chondrogenic-induced cells that are normally found in the cells of natural cartilage. All in all, this study manifested that AF can be a major consideration and applied for use as a co-mixture of cryogenic medium.
format Journal
author Suteera Narakornsak
Sirinda Aungsuchawan
Peraphan Pothacharoen
Chaniporn Puaninta
Runchana Markmee
Waleephan Tancharoen
Tanongsak Laowanitwattana
Naree Poovachiranon
Chawapon Thaojamnong
author_facet Suteera Narakornsak
Sirinda Aungsuchawan
Peraphan Pothacharoen
Chaniporn Puaninta
Runchana Markmee
Waleephan Tancharoen
Tanongsak Laowanitwattana
Naree Poovachiranon
Chawapon Thaojamnong
author_sort Suteera Narakornsak
title Amniotic fluid: Source of valuable mesenchymal stem cells and alternatively used as cryopreserved solution
title_short Amniotic fluid: Source of valuable mesenchymal stem cells and alternatively used as cryopreserved solution
title_full Amniotic fluid: Source of valuable mesenchymal stem cells and alternatively used as cryopreserved solution
title_fullStr Amniotic fluid: Source of valuable mesenchymal stem cells and alternatively used as cryopreserved solution
title_full_unstemmed Amniotic fluid: Source of valuable mesenchymal stem cells and alternatively used as cryopreserved solution
title_sort amniotic fluid: source of valuable mesenchymal stem cells and alternatively used as cryopreserved solution
publishDate 2018
url https://www.scopus.com/inward/record.uri?partnerID=HzOxMe3b&scp=85055911810&origin=inward
http://cmuir.cmu.ac.th/jspui/handle/6653943832/62592
_version_ 1681425835909709824