Oncogenic roles of serine–threonine kinase receptor-associated protein (STRAP) in osteosarcoma

© 2018, Springer-Verlag GmbH Germany, part of Springer Nature. Purpose: To validate the presence of serine–threonine kinase receptor-associated Protein (STRAP) in osteosarcoma tissue and to investigate the oncological role of STRAP in osteosarcoma. Methods: Expression of STRAP protein in osteosarcom...

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Main Authors: Dumnoensun Pruksakorn, Jeerawan Klangjorhor, Kriengsak Lirdprapamongkol, Pimpisa Teeyakasem, Patsadakorn Sungngam, Parunya Chaiyawat, Areerak Phanphaisarn, Jongkolnee Settakorn, Chantragan Srisomsap
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Published: 2018
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http://cmuir.cmu.ac.th/jspui/handle/6653943832/62594
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spelling th-cmuir.6653943832-625942018-11-29T07:56:37Z Oncogenic roles of serine–threonine kinase receptor-associated protein (STRAP) in osteosarcoma Dumnoensun Pruksakorn Jeerawan Klangjorhor Kriengsak Lirdprapamongkol Pimpisa Teeyakasem Patsadakorn Sungngam Parunya Chaiyawat Areerak Phanphaisarn Jongkolnee Settakorn Chantragan Srisomsap Biochemistry, Genetics and Molecular Biology Medicine Pharmacology, Toxicology and Pharmaceutics © 2018, Springer-Verlag GmbH Germany, part of Springer Nature. Purpose: To validate the presence of serine–threonine kinase receptor-associated Protein (STRAP) in osteosarcoma tissue and to investigate the oncological role of STRAP in osteosarcoma. Methods: Expression of STRAP protein in osteosarcoma tissue compared to soft callus (hyperactive bone healing tissue) and in multiple cell lines was examined using western blot analysis. Effects of STRAP silencing on cell proliferation, invasion, migration and re-implantability in chick chorioallantoic membrane (CAM) were observed in osteosarcoma cell lines (MNNG-HOS, 143B, and U2OS). Results: The result demonstrated that STRAP was highly up-regulated in osteosarcoma tissues compared with the normal physiological bone healing tissue (soft callus). Expression level of STRAP was markedly high in osteosarcoma cell lines with aggressive phenotype. Upon STRAP silencing, invasion and migration, but not proliferative activity, were selectively modulated in high-expression-STRAP cell lines. In addition, STRAP silencing reduced the success rate of tumor implantation and growth of MNNG-HOS cells in CAM model. Conclusions: Serine–threonine kinase receptor-associated protein is up-regulated during osteosarcoma progression. The presence of STRAP enhances osteosarcoma cell invasion, migration and re-implantation ability, factors which play a critical role in metastasis. Serine–threonine kinase receptor-associated protein and its related pathway are worthy for further exploration as a novel target for anti-metastasis agents. 2018-11-29T07:34:30Z 2018-11-29T07:34:30Z 2018-01-01 Journal 14320843 03445704 2-s2.0-85054302098 10.1007/s00280-018-3696-3 https://www.scopus.com/inward/record.uri?partnerID=HzOxMe3b&scp=85054302098&origin=inward http://cmuir.cmu.ac.th/jspui/handle/6653943832/62594
institution Chiang Mai University
building Chiang Mai University Library
country Thailand
collection CMU Intellectual Repository
topic Biochemistry, Genetics and Molecular Biology
Medicine
Pharmacology, Toxicology and Pharmaceutics
spellingShingle Biochemistry, Genetics and Molecular Biology
Medicine
Pharmacology, Toxicology and Pharmaceutics
Dumnoensun Pruksakorn
Jeerawan Klangjorhor
Kriengsak Lirdprapamongkol
Pimpisa Teeyakasem
Patsadakorn Sungngam
Parunya Chaiyawat
Areerak Phanphaisarn
Jongkolnee Settakorn
Chantragan Srisomsap
Oncogenic roles of serine–threonine kinase receptor-associated protein (STRAP) in osteosarcoma
description © 2018, Springer-Verlag GmbH Germany, part of Springer Nature. Purpose: To validate the presence of serine–threonine kinase receptor-associated Protein (STRAP) in osteosarcoma tissue and to investigate the oncological role of STRAP in osteosarcoma. Methods: Expression of STRAP protein in osteosarcoma tissue compared to soft callus (hyperactive bone healing tissue) and in multiple cell lines was examined using western blot analysis. Effects of STRAP silencing on cell proliferation, invasion, migration and re-implantability in chick chorioallantoic membrane (CAM) were observed in osteosarcoma cell lines (MNNG-HOS, 143B, and U2OS). Results: The result demonstrated that STRAP was highly up-regulated in osteosarcoma tissues compared with the normal physiological bone healing tissue (soft callus). Expression level of STRAP was markedly high in osteosarcoma cell lines with aggressive phenotype. Upon STRAP silencing, invasion and migration, but not proliferative activity, were selectively modulated in high-expression-STRAP cell lines. In addition, STRAP silencing reduced the success rate of tumor implantation and growth of MNNG-HOS cells in CAM model. Conclusions: Serine–threonine kinase receptor-associated protein is up-regulated during osteosarcoma progression. The presence of STRAP enhances osteosarcoma cell invasion, migration and re-implantation ability, factors which play a critical role in metastasis. Serine–threonine kinase receptor-associated protein and its related pathway are worthy for further exploration as a novel target for anti-metastasis agents.
format Journal
author Dumnoensun Pruksakorn
Jeerawan Klangjorhor
Kriengsak Lirdprapamongkol
Pimpisa Teeyakasem
Patsadakorn Sungngam
Parunya Chaiyawat
Areerak Phanphaisarn
Jongkolnee Settakorn
Chantragan Srisomsap
author_facet Dumnoensun Pruksakorn
Jeerawan Klangjorhor
Kriengsak Lirdprapamongkol
Pimpisa Teeyakasem
Patsadakorn Sungngam
Parunya Chaiyawat
Areerak Phanphaisarn
Jongkolnee Settakorn
Chantragan Srisomsap
author_sort Dumnoensun Pruksakorn
title Oncogenic roles of serine–threonine kinase receptor-associated protein (STRAP) in osteosarcoma
title_short Oncogenic roles of serine–threonine kinase receptor-associated protein (STRAP) in osteosarcoma
title_full Oncogenic roles of serine–threonine kinase receptor-associated protein (STRAP) in osteosarcoma
title_fullStr Oncogenic roles of serine–threonine kinase receptor-associated protein (STRAP) in osteosarcoma
title_full_unstemmed Oncogenic roles of serine–threonine kinase receptor-associated protein (STRAP) in osteosarcoma
title_sort oncogenic roles of serine–threonine kinase receptor-associated protein (strap) in osteosarcoma
publishDate 2018
url https://www.scopus.com/inward/record.uri?partnerID=HzOxMe3b&scp=85054302098&origin=inward
http://cmuir.cmu.ac.th/jspui/handle/6653943832/62594
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