Novel bioactive peptides demonstrating anti-dengue virus activity isolated from the Asian medicinal plant Acacia Catechu

© 2018 John Wiley & Sons A/S The therapeutic activities of food-derived bioactive proteins and peptides are attracting increased attention within the research community. Medicinal plants used in traditional medicines are an excellent source of bioactive proteins and peptides, especially those...

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Main Authors: Aussara Panya, Petlada Yongpitakwattana, Prapaphan Budchart, Nunghathai Sawasdee, Sucheewin Krobthong, Atchara Paemanee, Sittiruk Roytrakul, Siriluk Rattanabunyong, Kiattawee Choowongkomon, Pa thai Yenchitsomanus
Format: Journal
Published: 2018
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http://cmuir.cmu.ac.th/jspui/handle/6653943832/62599
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Institution: Chiang Mai University
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spelling th-cmuir.6653943832-625992018-11-29T07:56:43Z Novel bioactive peptides demonstrating anti-dengue virus activity isolated from the Asian medicinal plant Acacia Catechu Aussara Panya Petlada Yongpitakwattana Prapaphan Budchart Nunghathai Sawasdee Sucheewin Krobthong Atchara Paemanee Sittiruk Roytrakul Siriluk Rattanabunyong Kiattawee Choowongkomon Pa thai Yenchitsomanus Biochemistry, Genetics and Molecular Biology Chemistry Pharmacology, Toxicology and Pharmaceutics © 2018 John Wiley & Sons A/S The therapeutic activities of food-derived bioactive proteins and peptides are attracting increased attention within the research community. Medicinal plants used in traditional medicines are an excellent source of bioactive proteins and peptides, especially those traditionally prepared by water extraction for use as tea or food supplement. In this study, novel bioactive peptides were isolated from enzymatic digests of 33 Thai medicinal plants. The inhibitory activity of each against dengue virus (DENV) infection was investigated. Of 33 plants, peptides from Acacia catechu extract demonstrated the most pronounced anti-DENV activity. Half maximal inhibitory concentration of 0.18 μg/ml effectively inhibited DENV foci formation. Treatment with 1.25 μg/ml crude peptide extract could reduce virus production less than 100-fold with no observable cell toxicity. Peptide sequences were determined by high-performance liquid chromatography and liquid chromatography–tandem mass spectrometry. Two bioactive peptides isolated from Acacia catechu inhibited DENV foci formation >90% at the concentration of 50 μM; therefore, they are recommended for further investigation as antiviral peptides against DENV infection. 2018-11-29T07:34:51Z 2018-11-29T07:34:51Z 2018-01-01 Journal 17470285 17470277 2-s2.0-85054674941 10.1111/cbdd.13400 https://www.scopus.com/inward/record.uri?partnerID=HzOxMe3b&scp=85054674941&origin=inward http://cmuir.cmu.ac.th/jspui/handle/6653943832/62599
institution Chiang Mai University
building Chiang Mai University Library
country Thailand
collection CMU Intellectual Repository
topic Biochemistry, Genetics and Molecular Biology
Chemistry
Pharmacology, Toxicology and Pharmaceutics
spellingShingle Biochemistry, Genetics and Molecular Biology
Chemistry
Pharmacology, Toxicology and Pharmaceutics
Aussara Panya
Petlada Yongpitakwattana
Prapaphan Budchart
Nunghathai Sawasdee
Sucheewin Krobthong
Atchara Paemanee
Sittiruk Roytrakul
Siriluk Rattanabunyong
Kiattawee Choowongkomon
Pa thai Yenchitsomanus
Novel bioactive peptides demonstrating anti-dengue virus activity isolated from the Asian medicinal plant Acacia Catechu
description © 2018 John Wiley & Sons A/S The therapeutic activities of food-derived bioactive proteins and peptides are attracting increased attention within the research community. Medicinal plants used in traditional medicines are an excellent source of bioactive proteins and peptides, especially those traditionally prepared by water extraction for use as tea or food supplement. In this study, novel bioactive peptides were isolated from enzymatic digests of 33 Thai medicinal plants. The inhibitory activity of each against dengue virus (DENV) infection was investigated. Of 33 plants, peptides from Acacia catechu extract demonstrated the most pronounced anti-DENV activity. Half maximal inhibitory concentration of 0.18 μg/ml effectively inhibited DENV foci formation. Treatment with 1.25 μg/ml crude peptide extract could reduce virus production less than 100-fold with no observable cell toxicity. Peptide sequences were determined by high-performance liquid chromatography and liquid chromatography–tandem mass spectrometry. Two bioactive peptides isolated from Acacia catechu inhibited DENV foci formation >90% at the concentration of 50 μM; therefore, they are recommended for further investigation as antiviral peptides against DENV infection.
format Journal
author Aussara Panya
Petlada Yongpitakwattana
Prapaphan Budchart
Nunghathai Sawasdee
Sucheewin Krobthong
Atchara Paemanee
Sittiruk Roytrakul
Siriluk Rattanabunyong
Kiattawee Choowongkomon
Pa thai Yenchitsomanus
author_facet Aussara Panya
Petlada Yongpitakwattana
Prapaphan Budchart
Nunghathai Sawasdee
Sucheewin Krobthong
Atchara Paemanee
Sittiruk Roytrakul
Siriluk Rattanabunyong
Kiattawee Choowongkomon
Pa thai Yenchitsomanus
author_sort Aussara Panya
title Novel bioactive peptides demonstrating anti-dengue virus activity isolated from the Asian medicinal plant Acacia Catechu
title_short Novel bioactive peptides demonstrating anti-dengue virus activity isolated from the Asian medicinal plant Acacia Catechu
title_full Novel bioactive peptides demonstrating anti-dengue virus activity isolated from the Asian medicinal plant Acacia Catechu
title_fullStr Novel bioactive peptides demonstrating anti-dengue virus activity isolated from the Asian medicinal plant Acacia Catechu
title_full_unstemmed Novel bioactive peptides demonstrating anti-dengue virus activity isolated from the Asian medicinal plant Acacia Catechu
title_sort novel bioactive peptides demonstrating anti-dengue virus activity isolated from the asian medicinal plant acacia catechu
publishDate 2018
url https://www.scopus.com/inward/record.uri?partnerID=HzOxMe3b&scp=85054674941&origin=inward
http://cmuir.cmu.ac.th/jspui/handle/6653943832/62599
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