Triggering of CD99 on monocytes by a specific monoclonal antibody regulates T cell activation
© 2018 Elsevier Inc. CD99, a leukocyte surface glycoprotein, has been implicated in many cellular processes including cell adhesion, cell migration and T cell activation. Our previous study demonstrated the anti-CD99 monoclonal antibody (mAb) clone MT99/3 inhibited T cell activation; however, the me...
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th-cmuir.6653943832-627452018-11-29T07:45:22Z Triggering of CD99 on monocytes by a specific monoclonal antibody regulates T cell activation Witida Laopajon Supansa Pata Nuchjira Takheaw Sirirat Surinkaew Saichit Khummuang Watchara Kasinrerk Immunology and Microbiology © 2018 Elsevier Inc. CD99, a leukocyte surface glycoprotein, has been implicated in many cellular processes including cell adhesion, cell migration and T cell activation. Our previous study demonstrated the anti-CD99 monoclonal antibody (mAb) clone MT99/3 inhibited T cell activation; however, the mechanism is unclear. In this study, we demonstrated that CD99 expressed on monocytes played a role in the inhibition of T cell activation. Anti-CD99 mAb MT99/3 downregulated the expression of costimulatory molecule CD86, but upregulated IL-6, IL-10 and TNF-α production by monocytes. The inhibitory effect of mAb MT99/3 required cell to cell contact between monocytes and lymphocytes. The soluble mediators produced by monocytes alone were insufficient to induce hypo-function of T lymphocytes. In summary, we demonstrated that ligation of CD99 on monocytes by anti-CD99 mAb MT99/3 could mediate T cell hypo-responsiveness. These findings provide the first evidence of the role of CD99 on monocytes that contributes to T cell activation. 2018-11-29T07:45:22Z 2018-11-29T07:45:22Z 2018-01-01 Journal 10902163 00088749 2-s2.0-85055908810 10.1016/j.cellimm.2018.10.012 https://www.scopus.com/inward/record.uri?partnerID=HzOxMe3b&scp=85055908810&origin=inward http://cmuir.cmu.ac.th/jspui/handle/6653943832/62745 |
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Immunology and Microbiology Witida Laopajon Supansa Pata Nuchjira Takheaw Sirirat Surinkaew Saichit Khummuang Watchara Kasinrerk Triggering of CD99 on monocytes by a specific monoclonal antibody regulates T cell activation |
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© 2018 Elsevier Inc. CD99, a leukocyte surface glycoprotein, has been implicated in many cellular processes including cell adhesion, cell migration and T cell activation. Our previous study demonstrated the anti-CD99 monoclonal antibody (mAb) clone MT99/3 inhibited T cell activation; however, the mechanism is unclear. In this study, we demonstrated that CD99 expressed on monocytes played a role in the inhibition of T cell activation. Anti-CD99 mAb MT99/3 downregulated the expression of costimulatory molecule CD86, but upregulated IL-6, IL-10 and TNF-α production by monocytes. The inhibitory effect of mAb MT99/3 required cell to cell contact between monocytes and lymphocytes. The soluble mediators produced by monocytes alone were insufficient to induce hypo-function of T lymphocytes. In summary, we demonstrated that ligation of CD99 on monocytes by anti-CD99 mAb MT99/3 could mediate T cell hypo-responsiveness. These findings provide the first evidence of the role of CD99 on monocytes that contributes to T cell activation. |
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Witida Laopajon Supansa Pata Nuchjira Takheaw Sirirat Surinkaew Saichit Khummuang Watchara Kasinrerk |
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Witida Laopajon Supansa Pata Nuchjira Takheaw Sirirat Surinkaew Saichit Khummuang Watchara Kasinrerk |
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Witida Laopajon |
title |
Triggering of CD99 on monocytes by a specific monoclonal antibody regulates T cell activation |
title_short |
Triggering of CD99 on monocytes by a specific monoclonal antibody regulates T cell activation |
title_full |
Triggering of CD99 on monocytes by a specific monoclonal antibody regulates T cell activation |
title_fullStr |
Triggering of CD99 on monocytes by a specific monoclonal antibody regulates T cell activation |
title_full_unstemmed |
Triggering of CD99 on monocytes by a specific monoclonal antibody regulates T cell activation |
title_sort |
triggering of cd99 on monocytes by a specific monoclonal antibody regulates t cell activation |
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2018 |
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https://www.scopus.com/inward/record.uri?partnerID=HzOxMe3b&scp=85055908810&origin=inward http://cmuir.cmu.ac.th/jspui/handle/6653943832/62745 |
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1681425864294662144 |