Sensitive amperometric biosensors for detection of glucose and cholesterol using a platinum/reduced graphene oxide/poly(3-aminobenzoic acid) film-modified screen-printed carbon electrode
© 2019 A facile one-step electrochemical synthesis of a platinum/reduced graphene oxide/poly(3-aminobenzoic acid) (Pt/rGO/P3ABA) nanocomposite film on a screen-printed carbon electrode (SPCE) and its application in the development of sensitive amperometric biosensors was successfully demonstrated h...
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th-cmuir.6653943832-635642019-03-18T02:21:45Z Sensitive amperometric biosensors for detection of glucose and cholesterol using a platinum/reduced graphene oxide/poly(3-aminobenzoic acid) film-modified screen-printed carbon electrode Sopit Phetsang Jaroon Jakmunee Pitchaya Mungkornasawakul Rawiwan Laocharoensuk Kontad Ounnunkad Biochemistry, Genetics and Molecular Biology Chemistry © 2019 A facile one-step electrochemical synthesis of a platinum/reduced graphene oxide/poly(3-aminobenzoic acid) (Pt/rGO/P3ABA) nanocomposite film on a screen-printed carbon electrode (SPCE) and its application in the development of sensitive amperometric biosensors was successfully demonstrated herein. The electropolymerization of P3ABA together with co-electrodeposition of rGO and Pt was conducted by cyclic voltammetry, as was the GO reduction to rGO. A Pt/rGO/P3ABA-modified SPCE exhibited excellent electrocatalytic oxidation towards hydrogen peroxide (H 2 O 2 ) and can be employed as an electrochemical platform for the immobilization of glucose oxidase (GOx) and cholesterol oxidase (ChOx) to fabricate glucose and cholesterol biosensors, respectively. Under the optimized conditions at a working potential of +0.50 V, the proposed biosensors revealed excellent linear responses to glucose and cholesterol in the concentration ranges of 0.25–6.00 mM and 0.25–4.00 mM, respectively, with high sensitivities of 22.01 and 15.94 μA mM −1 cm −2 and low detection limits (LODs) of 44.3 and 40.5 μM. Additionally, the Michaelis-Menten constant (K m ) of GOx was 3.54 mM, while the K m of ChOx was 3.82 mM. Both biosensors displayed a good anti-interference ability and clearly exhibited acceptable recoveries for the detection of glucose and cholesterol in a human serum sample (98.2–104.1%). 2019-03-18T02:20:56Z 2019-03-18T02:20:56Z 2019-06-01 Journal 1878562X 15675394 2-s2.0-85061978502 10.1016/j.bioelechem.2019.01.008 https://www.scopus.com/inward/record.uri?partnerID=HzOxMe3b&scp=85061978502&origin=inward http://cmuir.cmu.ac.th/jspui/handle/6653943832/63564 |
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Biochemistry, Genetics and Molecular Biology Chemistry Sopit Phetsang Jaroon Jakmunee Pitchaya Mungkornasawakul Rawiwan Laocharoensuk Kontad Ounnunkad Sensitive amperometric biosensors for detection of glucose and cholesterol using a platinum/reduced graphene oxide/poly(3-aminobenzoic acid) film-modified screen-printed carbon electrode |
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© 2019 A facile one-step electrochemical synthesis of a platinum/reduced graphene oxide/poly(3-aminobenzoic acid) (Pt/rGO/P3ABA) nanocomposite film on a screen-printed carbon electrode (SPCE) and its application in the development of sensitive amperometric biosensors was successfully demonstrated herein. The electropolymerization of P3ABA together with co-electrodeposition of rGO and Pt was conducted by cyclic voltammetry, as was the GO reduction to rGO. A Pt/rGO/P3ABA-modified SPCE exhibited excellent electrocatalytic oxidation towards hydrogen peroxide (H 2 O 2 ) and can be employed as an electrochemical platform for the immobilization of glucose oxidase (GOx) and cholesterol oxidase (ChOx) to fabricate glucose and cholesterol biosensors, respectively. Under the optimized conditions at a working potential of +0.50 V, the proposed biosensors revealed excellent linear responses to glucose and cholesterol in the concentration ranges of 0.25–6.00 mM and 0.25–4.00 mM, respectively, with high sensitivities of 22.01 and 15.94 μA mM −1 cm −2 and low detection limits (LODs) of 44.3 and 40.5 μM. Additionally, the Michaelis-Menten constant (K m ) of GOx was 3.54 mM, while the K m of ChOx was 3.82 mM. Both biosensors displayed a good anti-interference ability and clearly exhibited acceptable recoveries for the detection of glucose and cholesterol in a human serum sample (98.2–104.1%). |
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Sopit Phetsang Jaroon Jakmunee Pitchaya Mungkornasawakul Rawiwan Laocharoensuk Kontad Ounnunkad |
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Sopit Phetsang Jaroon Jakmunee Pitchaya Mungkornasawakul Rawiwan Laocharoensuk Kontad Ounnunkad |
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Sopit Phetsang |
title |
Sensitive amperometric biosensors for detection of glucose and cholesterol using a platinum/reduced graphene oxide/poly(3-aminobenzoic acid) film-modified screen-printed carbon electrode |
title_short |
Sensitive amperometric biosensors for detection of glucose and cholesterol using a platinum/reduced graphene oxide/poly(3-aminobenzoic acid) film-modified screen-printed carbon electrode |
title_full |
Sensitive amperometric biosensors for detection of glucose and cholesterol using a platinum/reduced graphene oxide/poly(3-aminobenzoic acid) film-modified screen-printed carbon electrode |
title_fullStr |
Sensitive amperometric biosensors for detection of glucose and cholesterol using a platinum/reduced graphene oxide/poly(3-aminobenzoic acid) film-modified screen-printed carbon electrode |
title_full_unstemmed |
Sensitive amperometric biosensors for detection of glucose and cholesterol using a platinum/reduced graphene oxide/poly(3-aminobenzoic acid) film-modified screen-printed carbon electrode |
title_sort |
sensitive amperometric biosensors for detection of glucose and cholesterol using a platinum/reduced graphene oxide/poly(3-aminobenzoic acid) film-modified screen-printed carbon electrode |
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2019 |
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https://www.scopus.com/inward/record.uri?partnerID=HzOxMe3b&scp=85061978502&origin=inward http://cmuir.cmu.ac.th/jspui/handle/6653943832/63564 |
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