Enhanced mucosal immune responses, immune related genes and growth performance in common carp (Cyprinus carpio) juveniles fed dietary Pediococcus acidilactici MA18/5M and raffinose

© 2019 Elsevier Ltd The present study was conducted to evaluate the effects of dietary Pediococcus acidilactici (PA) and raffinose (RF) alone or in combination on growth performance, mucosal immune factors and immune related genes expression in common carp (Cyprinus carpio) juveniles. Fish with ini...

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Main Authors: Seyed Hossein Hoseinifar, Marjan Hosseini, Hamed Paknejad, Roghieh Safari, Ali Jafar, Morteza Yousefi, Hien Van Doan, Mansour Torfi Mozanzadeh
Format: Journal
Published: 2019
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http://cmuir.cmu.ac.th/jspui/handle/6653943832/63569
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spelling th-cmuir.6653943832-635692019-03-18T02:23:21Z Enhanced mucosal immune responses, immune related genes and growth performance in common carp (Cyprinus carpio) juveniles fed dietary Pediococcus acidilactici MA18/5M and raffinose Seyed Hossein Hoseinifar Marjan Hosseini Hamed Paknejad Roghieh Safari Ali Jafar Morteza Yousefi Hien Van Doan Mansour Torfi Mozanzadeh Biochemistry, Genetics and Molecular Biology Immunology and Microbiology © 2019 Elsevier Ltd The present study was conducted to evaluate the effects of dietary Pediococcus acidilactici (PA) and raffinose (RF) alone or in combination on growth performance, mucosal immune factors and immune related genes expression in common carp (Cyprinus carpio) juveniles. Fish with initial weight of 10.0 ± 2.5 g were fed the following experimental diets for 60 days: control (without supplementation), prebiotic (2 g RF kg −1 diet), probiotic (6 × 10 8 CFU g −1 PA) and synbiotic (2 g RF kg −1 + 6 × 10 8 CFU g −1 PA). Carp fed synbiotic and probiotic diets had the highest (19.53 ± 0.16) and the lowest (18.05 ± 0.65) final body weight, respectively and the other experimental groups showed intermediate values. Singular administration of PA or in combination with 2 g RF kg −1 significantly increased skin mucus total immunoglobulin (Ig) and protein compared other groups, meanwhile, values of skin mucus protease activity enhanced by dietary immunostimulants administration in comparison with the control (P < 0.05). The expression of gene encoding lysozyme in skin pronouncedly increased by supplementing diets with singular or mixture of PA and RF; however, the expression of intestinal lysozyme gene as well as tumor necrosis factor-α genes expression in skin and intestine were not affected by administrating different immunostimulants (P > 0.05). The highest growth performance was noticed in fish fed synbiotic (P < 0.05). Overall, the combination of 2 g RF kg −1 with 6 × 10 8 CFU g −1 PA is recommended for improving immunological responses of C. carpio juveniles. 2019-03-18T02:21:00Z 2019-03-18T02:21:00Z 2019-05-01 Journal 18790089 0145305X 2-s2.0-85061344587 10.1016/j.dci.2019.01.009 https://www.scopus.com/inward/record.uri?partnerID=HzOxMe3b&scp=85061344587&origin=inward http://cmuir.cmu.ac.th/jspui/handle/6653943832/63569
institution Chiang Mai University
building Chiang Mai University Library
country Thailand
collection CMU Intellectual Repository
topic Biochemistry, Genetics and Molecular Biology
Immunology and Microbiology
spellingShingle Biochemistry, Genetics and Molecular Biology
Immunology and Microbiology
Seyed Hossein Hoseinifar
Marjan Hosseini
Hamed Paknejad
Roghieh Safari
Ali Jafar
Morteza Yousefi
Hien Van Doan
Mansour Torfi Mozanzadeh
Enhanced mucosal immune responses, immune related genes and growth performance in common carp (Cyprinus carpio) juveniles fed dietary Pediococcus acidilactici MA18/5M and raffinose
description © 2019 Elsevier Ltd The present study was conducted to evaluate the effects of dietary Pediococcus acidilactici (PA) and raffinose (RF) alone or in combination on growth performance, mucosal immune factors and immune related genes expression in common carp (Cyprinus carpio) juveniles. Fish with initial weight of 10.0 ± 2.5 g were fed the following experimental diets for 60 days: control (without supplementation), prebiotic (2 g RF kg −1 diet), probiotic (6 × 10 8 CFU g −1 PA) and synbiotic (2 g RF kg −1 + 6 × 10 8 CFU g −1 PA). Carp fed synbiotic and probiotic diets had the highest (19.53 ± 0.16) and the lowest (18.05 ± 0.65) final body weight, respectively and the other experimental groups showed intermediate values. Singular administration of PA or in combination with 2 g RF kg −1 significantly increased skin mucus total immunoglobulin (Ig) and protein compared other groups, meanwhile, values of skin mucus protease activity enhanced by dietary immunostimulants administration in comparison with the control (P < 0.05). The expression of gene encoding lysozyme in skin pronouncedly increased by supplementing diets with singular or mixture of PA and RF; however, the expression of intestinal lysozyme gene as well as tumor necrosis factor-α genes expression in skin and intestine were not affected by administrating different immunostimulants (P > 0.05). The highest growth performance was noticed in fish fed synbiotic (P < 0.05). Overall, the combination of 2 g RF kg −1 with 6 × 10 8 CFU g −1 PA is recommended for improving immunological responses of C. carpio juveniles.
format Journal
author Seyed Hossein Hoseinifar
Marjan Hosseini
Hamed Paknejad
Roghieh Safari
Ali Jafar
Morteza Yousefi
Hien Van Doan
Mansour Torfi Mozanzadeh
author_facet Seyed Hossein Hoseinifar
Marjan Hosseini
Hamed Paknejad
Roghieh Safari
Ali Jafar
Morteza Yousefi
Hien Van Doan
Mansour Torfi Mozanzadeh
author_sort Seyed Hossein Hoseinifar
title Enhanced mucosal immune responses, immune related genes and growth performance in common carp (Cyprinus carpio) juveniles fed dietary Pediococcus acidilactici MA18/5M and raffinose
title_short Enhanced mucosal immune responses, immune related genes and growth performance in common carp (Cyprinus carpio) juveniles fed dietary Pediococcus acidilactici MA18/5M and raffinose
title_full Enhanced mucosal immune responses, immune related genes and growth performance in common carp (Cyprinus carpio) juveniles fed dietary Pediococcus acidilactici MA18/5M and raffinose
title_fullStr Enhanced mucosal immune responses, immune related genes and growth performance in common carp (Cyprinus carpio) juveniles fed dietary Pediococcus acidilactici MA18/5M and raffinose
title_full_unstemmed Enhanced mucosal immune responses, immune related genes and growth performance in common carp (Cyprinus carpio) juveniles fed dietary Pediococcus acidilactici MA18/5M and raffinose
title_sort enhanced mucosal immune responses, immune related genes and growth performance in common carp (cyprinus carpio) juveniles fed dietary pediococcus acidilactici ma18/5m and raffinose
publishDate 2019
url https://www.scopus.com/inward/record.uri?partnerID=HzOxMe3b&scp=85061344587&origin=inward
http://cmuir.cmu.ac.th/jspui/handle/6653943832/63569
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