Inhibition of dengue virus replication in monocyte-derived dendritic cells by vivo-morpholino oligomers

© 2018 Elsevier B.V. Skin dendritic cells (DCs) are primary target cells of dengue virus (DENV) infection and they play an important role in its immunopathogenesis. Monocyte-derived dendritic cells (MDDCs) represent dermal and bloodstream DCs that serve as human primary cells for ex vivo studies of...

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Main Authors: Patta Phumesin, Mutita Junking, Aussara Panya, Petlada Yongpitakwattana, Sansanee Noisakran, Thawornchai Limjindaporn, Pa thai Yenchitsomanus
Format: Journal
Published: 2019
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http://cmuir.cmu.ac.th/jspui/handle/6653943832/63580
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spelling th-cmuir.6653943832-635802019-03-18T02:24:39Z Inhibition of dengue virus replication in monocyte-derived dendritic cells by vivo-morpholino oligomers Patta Phumesin Mutita Junking Aussara Panya Petlada Yongpitakwattana Sansanee Noisakran Thawornchai Limjindaporn Pa thai Yenchitsomanus Biochemistry, Genetics and Molecular Biology Immunology and Microbiology Medicine © 2018 Elsevier B.V. Skin dendritic cells (DCs) are primary target cells of dengue virus (DENV) infection and they play an important role in its immunopathogenesis. Monocyte-derived dendritic cells (MDDCs) represent dermal and bloodstream DCs that serve as human primary cells for ex vivo studies of DENV infection. Improved understanding of the mechanisms that effectuate the inhibition of DENV replication in MDDCs will accelerate the development of antiviral drugs to treat DENV infection. In this study, we investigated whether or not vivo-morpholino oligomer (vivo-MO), which was designed to target the top of the 3′ stem-loop (3′ SL) at the 3′ UTR of the DENV genome, could inhibit DENV infection and replication in MDDCs. The findings of this study revealed that vivo-MO-1 could inhibit DENV-2 infection in MDDCs, and that it could significantly reduce DENV RNA, protein, and viral production in a dose-dependent manner. Treatment of MDDCs with 4 μM of vivo-MO-1 decreased DENV production by more than 1,000-fold, when compared to that of the vivo-MO-NC control. Thus, vivo-MO-1 targeting of DENV RNA demonstrates potential for further development into an anti-DENV agent. 2019-03-18T02:21:11Z 2019-03-18T02:21:11Z 2019-01-15 Journal 18727492 01681702 2-s2.0-85058065238 10.1016/j.virusres.2018.11.014 https://www.scopus.com/inward/record.uri?partnerID=HzOxMe3b&scp=85058065238&origin=inward http://cmuir.cmu.ac.th/jspui/handle/6653943832/63580
institution Chiang Mai University
building Chiang Mai University Library
country Thailand
collection CMU Intellectual Repository
topic Biochemistry, Genetics and Molecular Biology
Immunology and Microbiology
Medicine
spellingShingle Biochemistry, Genetics and Molecular Biology
Immunology and Microbiology
Medicine
Patta Phumesin
Mutita Junking
Aussara Panya
Petlada Yongpitakwattana
Sansanee Noisakran
Thawornchai Limjindaporn
Pa thai Yenchitsomanus
Inhibition of dengue virus replication in monocyte-derived dendritic cells by vivo-morpholino oligomers
description © 2018 Elsevier B.V. Skin dendritic cells (DCs) are primary target cells of dengue virus (DENV) infection and they play an important role in its immunopathogenesis. Monocyte-derived dendritic cells (MDDCs) represent dermal and bloodstream DCs that serve as human primary cells for ex vivo studies of DENV infection. Improved understanding of the mechanisms that effectuate the inhibition of DENV replication in MDDCs will accelerate the development of antiviral drugs to treat DENV infection. In this study, we investigated whether or not vivo-morpholino oligomer (vivo-MO), which was designed to target the top of the 3′ stem-loop (3′ SL) at the 3′ UTR of the DENV genome, could inhibit DENV infection and replication in MDDCs. The findings of this study revealed that vivo-MO-1 could inhibit DENV-2 infection in MDDCs, and that it could significantly reduce DENV RNA, protein, and viral production in a dose-dependent manner. Treatment of MDDCs with 4 μM of vivo-MO-1 decreased DENV production by more than 1,000-fold, when compared to that of the vivo-MO-NC control. Thus, vivo-MO-1 targeting of DENV RNA demonstrates potential for further development into an anti-DENV agent.
format Journal
author Patta Phumesin
Mutita Junking
Aussara Panya
Petlada Yongpitakwattana
Sansanee Noisakran
Thawornchai Limjindaporn
Pa thai Yenchitsomanus
author_facet Patta Phumesin
Mutita Junking
Aussara Panya
Petlada Yongpitakwattana
Sansanee Noisakran
Thawornchai Limjindaporn
Pa thai Yenchitsomanus
author_sort Patta Phumesin
title Inhibition of dengue virus replication in monocyte-derived dendritic cells by vivo-morpholino oligomers
title_short Inhibition of dengue virus replication in monocyte-derived dendritic cells by vivo-morpholino oligomers
title_full Inhibition of dengue virus replication in monocyte-derived dendritic cells by vivo-morpholino oligomers
title_fullStr Inhibition of dengue virus replication in monocyte-derived dendritic cells by vivo-morpholino oligomers
title_full_unstemmed Inhibition of dengue virus replication in monocyte-derived dendritic cells by vivo-morpholino oligomers
title_sort inhibition of dengue virus replication in monocyte-derived dendritic cells by vivo-morpholino oligomers
publishDate 2019
url https://www.scopus.com/inward/record.uri?partnerID=HzOxMe3b&scp=85058065238&origin=inward
http://cmuir.cmu.ac.th/jspui/handle/6653943832/63580
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