Partial Purification and Comparison of Precipitation Techniques of Pyruvate Decarboxylase Enzyme

• Main Authors: Julaluk Tangtua, Charin Techapun, Ronachai Pratanaphon, Ampin Kuntiya, Vorapat Sanguanchaipaiwong, Thanongsak Chaiyaso, Prasert Hanmoungjai, Phisit Seesuriyachan, Nopphorn Leksawasdi, Noppol Leksawasdi
• Format: บทความวารสาร
• Language: English
• Published: Science Faculty of Chiang Mai University 2019
• Online Access: http://it.science.cmu.ac.th/ejournal/dl.php?journal_id=7671; http://cmuir.cmu.ac.th/jspui/handle/6653943832/63844
• Institution: Chiang Mai University

The intracellular pyruvate decarboxylase enzyme (PDC, EC 4.1.1.1) extract from Candida tropicalis TISTR 5350 was compared by two different purification methods using ammonium sulphate and acetone precipitation. The total volumetric PDC activity and percentage recovery (yield) of precipitated PDC ba...