The Establishment and Activity of Bacterial Inoculant Immobilized in Agar Compared with Those of Alginate and Perlite After Being Introduced Into Soil

The objectives of this study were to select effective bacteria that produced high amounts of IAA and ammonia, and to determine the potential of using agar compared with alginate and perlite as cell immobilization materials to maintain a long survival of the inoculant strains. The Ensifer spp. strain...

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Main Authors: Pongrawee Nimnoi, Neelawan Pongsilp, Saisamorn Lumyong
Format: บทความวารสาร
Language:English
Published: Science Faculty of Chiang Mai University 2019
Online Access:http://it.science.cmu.ac.th/ejournal/dl.php?journal_id=8265
http://cmuir.cmu.ac.th/jspui/handle/6653943832/63901
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Institution: Chiang Mai University
Language: English
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spelling th-cmuir.6653943832-639012019-05-07T09:59:37Z The Establishment and Activity of Bacterial Inoculant Immobilized in Agar Compared with Those of Alginate and Perlite After Being Introduced Into Soil Pongrawee Nimnoi Neelawan Pongsilp Saisamorn Lumyong The objectives of this study were to select effective bacteria that produced high amounts of IAA and ammonia, and to determine the potential of using agar compared with alginate and perlite as cell immobilization materials to maintain a long survival of the inoculant strains. The Ensifer spp. strains LP2/20, NK2/9 and Bacillus sp. strain NK2/17 were selected, immobilized and stored at 30 °C for 15 days. After 15 days of storage, Ensifer sp. strain LP2/20 immobilized in agar exhibited the highest number of bacterial survival which was significantly different from those of other materials. In pot experiments, the establishment and activity of the inoculant after 15 days of introducing into soil were investigated by PCR-denaturing gradient gel electrophoresis (DGGE) and Reverse Transcription (RT) PCR-DGGE. Cloning and sequencing of 16S rRNA gene fragments obtained from DNA- and RNA-based DGGE gels confirmed that agar and alginate were the preferable immobilization materials that prolonged the survival times and supported the establishment of the inoculant after being introduced into soil rather than absorption in perlite. The inoculant immobilized in agar and alginate were still metabolically active after 15 days of introducing into soil, while usage of perlite as a carrier and liquid inoculation were proved ineffective in maintaining the activity of the inoculant. Moreover, inoculation with either immobilization material exhibited the potential to change bacterial community structure in soil. 2019-05-07T09:59:37Z 2019-05-07T09:59:37Z 2017 บทความวารสาร 0125-2526 http://it.science.cmu.ac.th/ejournal/dl.php?journal_id=8265 http://cmuir.cmu.ac.th/jspui/handle/6653943832/63901 Eng Science Faculty of Chiang Mai University
institution Chiang Mai University
building Chiang Mai University Library
country Thailand
collection CMU Intellectual Repository
language English
description The objectives of this study were to select effective bacteria that produced high amounts of IAA and ammonia, and to determine the potential of using agar compared with alginate and perlite as cell immobilization materials to maintain a long survival of the inoculant strains. The Ensifer spp. strains LP2/20, NK2/9 and Bacillus sp. strain NK2/17 were selected, immobilized and stored at 30 °C for 15 days. After 15 days of storage, Ensifer sp. strain LP2/20 immobilized in agar exhibited the highest number of bacterial survival which was significantly different from those of other materials. In pot experiments, the establishment and activity of the inoculant after 15 days of introducing into soil were investigated by PCR-denaturing gradient gel electrophoresis (DGGE) and Reverse Transcription (RT) PCR-DGGE. Cloning and sequencing of 16S rRNA gene fragments obtained from DNA- and RNA-based DGGE gels confirmed that agar and alginate were the preferable immobilization materials that prolonged the survival times and supported the establishment of the inoculant after being introduced into soil rather than absorption in perlite. The inoculant immobilized in agar and alginate were still metabolically active after 15 days of introducing into soil, while usage of perlite as a carrier and liquid inoculation were proved ineffective in maintaining the activity of the inoculant. Moreover, inoculation with either immobilization material exhibited the potential to change bacterial community structure in soil.
format บทความวารสาร
author Pongrawee Nimnoi
Neelawan Pongsilp
Saisamorn Lumyong
spellingShingle Pongrawee Nimnoi
Neelawan Pongsilp
Saisamorn Lumyong
The Establishment and Activity of Bacterial Inoculant Immobilized in Agar Compared with Those of Alginate and Perlite After Being Introduced Into Soil
author_facet Pongrawee Nimnoi
Neelawan Pongsilp
Saisamorn Lumyong
author_sort Pongrawee Nimnoi
title The Establishment and Activity of Bacterial Inoculant Immobilized in Agar Compared with Those of Alginate and Perlite After Being Introduced Into Soil
title_short The Establishment and Activity of Bacterial Inoculant Immobilized in Agar Compared with Those of Alginate and Perlite After Being Introduced Into Soil
title_full The Establishment and Activity of Bacterial Inoculant Immobilized in Agar Compared with Those of Alginate and Perlite After Being Introduced Into Soil
title_fullStr The Establishment and Activity of Bacterial Inoculant Immobilized in Agar Compared with Those of Alginate and Perlite After Being Introduced Into Soil
title_full_unstemmed The Establishment and Activity of Bacterial Inoculant Immobilized in Agar Compared with Those of Alginate and Perlite After Being Introduced Into Soil
title_sort establishment and activity of bacterial inoculant immobilized in agar compared with those of alginate and perlite after being introduced into soil
publisher Science Faculty of Chiang Mai University
publishDate 2019
url http://it.science.cmu.ac.th/ejournal/dl.php?journal_id=8265
http://cmuir.cmu.ac.th/jspui/handle/6653943832/63901
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