Flow Injection Amperometric Detection and Off-line Sequential Extraction Column for Fractionation of Phosphorus in Sediment

Flow injection amperometric system has been developed for determination of phosphorus. It is based on in-line formation of 12-molybdophosphate complex, which is further electrochemically reduced on a glassy carbon working electrode at a constant applied potential of 0.20 V vs. Ag/AgCl. An electrical...

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Main Authors: Pimporn Insain, Jaroon Jakmunee
Format: บทความวารสาร
Language:English
Published: Science Faculty of Chiang Mai University 2019
Online Access:http://it.science.cmu.ac.th/ejournal/dl.php?journal_id=8284
http://cmuir.cmu.ac.th/jspui/handle/6653943832/63920
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Institution: Chiang Mai University
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spelling th-cmuir.6653943832-639202019-05-07T09:59:38Z Flow Injection Amperometric Detection and Off-line Sequential Extraction Column for Fractionation of Phosphorus in Sediment Pimporn Insain Jaroon Jakmunee Flow injection amperometric system has been developed for determination of phosphorus. It is based on in-line formation of 12-molybdophosphate complex, which is further electrochemically reduced on a glassy carbon working electrode at a constant applied potential of 0.20 V vs. Ag/AgCl. An electrical current is directly proportional to phosphate concentration. Calibration graphs plotting between peak height and phosphate concentration were obtained in range of 0.1-1.0 and 1.0-10.0 mg L-1 P-PO4, with a detection limit of 0.03 mg L-1 P-PO4. The precision of 2.1, 2.6, 1.5, 1.2 and 1.0% (n=11, 0.1, 0.5, 1.0, 5.0 and 10.0 mg L-1 P-PO4, respectively) and sample throughput of 90 h-1 were achieved. The method has lower interferences (i.e., from silicate, colored and colloidal substances, and the Schlieren’s effect) than the commonly used spectrophotometric method. Although it may be interfered by cations, e.g., Fe3+ and Al3+, these ions could be eliminated by using Chelex 100 resin column. The proposed system was successfully applied to determine phosphorus that sequentially leached from sediments. An off-line leaching column was proposed for fractionation of phosphorus according to Hieltjes-Lijklema scheme (HLS), employing 1.0 M NH4Cl, 0.1 M NaOH, and 0.5 M HCl as extractants, respectively. The results obtained from extraction by using the proposed column method and the batch shaking method were in good agreement (no significant difference at 95% confident level). 2019-05-07T09:59:38Z 2019-05-07T09:59:38Z 2017 บทความวารสาร 0125-2526 http://it.science.cmu.ac.th/ejournal/dl.php?journal_id=8284 http://cmuir.cmu.ac.th/jspui/handle/6653943832/63920 Eng Science Faculty of Chiang Mai University
institution Chiang Mai University
building Chiang Mai University Library
country Thailand
collection CMU Intellectual Repository
language English
description Flow injection amperometric system has been developed for determination of phosphorus. It is based on in-line formation of 12-molybdophosphate complex, which is further electrochemically reduced on a glassy carbon working electrode at a constant applied potential of 0.20 V vs. Ag/AgCl. An electrical current is directly proportional to phosphate concentration. Calibration graphs plotting between peak height and phosphate concentration were obtained in range of 0.1-1.0 and 1.0-10.0 mg L-1 P-PO4, with a detection limit of 0.03 mg L-1 P-PO4. The precision of 2.1, 2.6, 1.5, 1.2 and 1.0% (n=11, 0.1, 0.5, 1.0, 5.0 and 10.0 mg L-1 P-PO4, respectively) and sample throughput of 90 h-1 were achieved. The method has lower interferences (i.e., from silicate, colored and colloidal substances, and the Schlieren’s effect) than the commonly used spectrophotometric method. Although it may be interfered by cations, e.g., Fe3+ and Al3+, these ions could be eliminated by using Chelex 100 resin column. The proposed system was successfully applied to determine phosphorus that sequentially leached from sediments. An off-line leaching column was proposed for fractionation of phosphorus according to Hieltjes-Lijklema scheme (HLS), employing 1.0 M NH4Cl, 0.1 M NaOH, and 0.5 M HCl as extractants, respectively. The results obtained from extraction by using the proposed column method and the batch shaking method were in good agreement (no significant difference at 95% confident level).
format บทความวารสาร
author Pimporn Insain
Jaroon Jakmunee
spellingShingle Pimporn Insain
Jaroon Jakmunee
Flow Injection Amperometric Detection and Off-line Sequential Extraction Column for Fractionation of Phosphorus in Sediment
author_facet Pimporn Insain
Jaroon Jakmunee
author_sort Pimporn Insain
title Flow Injection Amperometric Detection and Off-line Sequential Extraction Column for Fractionation of Phosphorus in Sediment
title_short Flow Injection Amperometric Detection and Off-line Sequential Extraction Column for Fractionation of Phosphorus in Sediment
title_full Flow Injection Amperometric Detection and Off-line Sequential Extraction Column for Fractionation of Phosphorus in Sediment
title_fullStr Flow Injection Amperometric Detection and Off-line Sequential Extraction Column for Fractionation of Phosphorus in Sediment
title_full_unstemmed Flow Injection Amperometric Detection and Off-line Sequential Extraction Column for Fractionation of Phosphorus in Sediment
title_sort flow injection amperometric detection and off-line sequential extraction column for fractionation of phosphorus in sediment
publisher Science Faculty of Chiang Mai University
publishDate 2019
url http://it.science.cmu.ac.th/ejournal/dl.php?journal_id=8284
http://cmuir.cmu.ac.th/jspui/handle/6653943832/63920
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