In vitro Anti-cancer Activity Comparison of the Freeze-dried and Spray-dried Bromelain from Pineapple Stems

Bromelain is one of the proteolytic enzymes found in pineapple stems and fruits. It has been investigated for medical uses including anti-cancer activity. The objective of this study was to compare the in vitro anti-cancer activities of the freeze-dried and spray-dried bromelain from pineapple stems...

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Main Authors: Worapaka Manosroi, Charinya Chankhampan, Jiradej Manosroi, Aranya Manosroi
Format: บทความวารสาร
Language:English
Published: Science Faculty of Chiang Mai University 2019
Online Access:http://it.science.cmu.ac.th/ejournal/dl.php?journal_id=8468
http://cmuir.cmu.ac.th/jspui/handle/6653943832/63964
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spelling th-cmuir.6653943832-639642019-05-07T09:59:40Z In vitro Anti-cancer Activity Comparison of the Freeze-dried and Spray-dried Bromelain from Pineapple Stems Worapaka Manosroi Charinya Chankhampan Jiradej Manosroi Aranya Manosroi Bromelain is one of the proteolytic enzymes found in pineapple stems and fruits. It has been investigated for medical uses including anti-cancer activity. The objective of this study was to compare the in vitro anti-cancer activities of the freeze-dried and spray-dried bromelain from pineapple stems. Bromelain was precipitated from the pineapple stem juice by 95%v/v ethanol and then freeze-dried or spray-dried to get the dry powder. Both bromelain samples were investigated for anti-cancer activities including anti-proliferative by sulforhodamine B (SRB) assay and apoptotic induction by acridine orange (AO) and ethidium bromide (EB) double staining on seven human cancer cell lines. The highest anti-proliferation of the freeze-dried and the spray-dried bromelain was observed on A549 with the IC50 values of 18.31±5.11 and 26.36±9.76 mg/ml, respectively. For apoptotic induction, the highest activity of the freeze-dried and the spray-dried bromelain was observed on KB (7.53±3.51%) and A549 (6.29±0.28%) cell lines, respectively. The freeze-dried and the spray-dried stem bromelain exhibited higher immunostimulating activity of the bromelain samples performed in murine neutrophils using nitroblue tetrazolium (NBT) dye reduction test than the negative control of 4.37 and 4.40 times, respectively. This study has demonstrated not only the no significant different effect of the drying processes on the anti-cancer activities of the stem bromelain in seven cancer cell lines, but also the potential of the stem bromelain to be developed as an active compound for lung cancer because of its highest anti-proliferation and apoptosis induction in A549. 2019-05-07T09:59:40Z 2019-05-07T09:59:40Z 2017 บทความวารสาร 0125-2526 http://it.science.cmu.ac.th/ejournal/dl.php?journal_id=8468 http://cmuir.cmu.ac.th/jspui/handle/6653943832/63964 Eng Science Faculty of Chiang Mai University
institution Chiang Mai University
building Chiang Mai University Library
country Thailand
collection CMU Intellectual Repository
language English
description Bromelain is one of the proteolytic enzymes found in pineapple stems and fruits. It has been investigated for medical uses including anti-cancer activity. The objective of this study was to compare the in vitro anti-cancer activities of the freeze-dried and spray-dried bromelain from pineapple stems. Bromelain was precipitated from the pineapple stem juice by 95%v/v ethanol and then freeze-dried or spray-dried to get the dry powder. Both bromelain samples were investigated for anti-cancer activities including anti-proliferative by sulforhodamine B (SRB) assay and apoptotic induction by acridine orange (AO) and ethidium bromide (EB) double staining on seven human cancer cell lines. The highest anti-proliferation of the freeze-dried and the spray-dried bromelain was observed on A549 with the IC50 values of 18.31±5.11 and 26.36±9.76 mg/ml, respectively. For apoptotic induction, the highest activity of the freeze-dried and the spray-dried bromelain was observed on KB (7.53±3.51%) and A549 (6.29±0.28%) cell lines, respectively. The freeze-dried and the spray-dried stem bromelain exhibited higher immunostimulating activity of the bromelain samples performed in murine neutrophils using nitroblue tetrazolium (NBT) dye reduction test than the negative control of 4.37 and 4.40 times, respectively. This study has demonstrated not only the no significant different effect of the drying processes on the anti-cancer activities of the stem bromelain in seven cancer cell lines, but also the potential of the stem bromelain to be developed as an active compound for lung cancer because of its highest anti-proliferation and apoptosis induction in A549.
format บทความวารสาร
author Worapaka Manosroi
Charinya Chankhampan
Jiradej Manosroi
Aranya Manosroi
spellingShingle Worapaka Manosroi
Charinya Chankhampan
Jiradej Manosroi
Aranya Manosroi
In vitro Anti-cancer Activity Comparison of the Freeze-dried and Spray-dried Bromelain from Pineapple Stems
author_facet Worapaka Manosroi
Charinya Chankhampan
Jiradej Manosroi
Aranya Manosroi
author_sort Worapaka Manosroi
title In vitro Anti-cancer Activity Comparison of the Freeze-dried and Spray-dried Bromelain from Pineapple Stems
title_short In vitro Anti-cancer Activity Comparison of the Freeze-dried and Spray-dried Bromelain from Pineapple Stems
title_full In vitro Anti-cancer Activity Comparison of the Freeze-dried and Spray-dried Bromelain from Pineapple Stems
title_fullStr In vitro Anti-cancer Activity Comparison of the Freeze-dried and Spray-dried Bromelain from Pineapple Stems
title_full_unstemmed In vitro Anti-cancer Activity Comparison of the Freeze-dried and Spray-dried Bromelain from Pineapple Stems
title_sort in vitro anti-cancer activity comparison of the freeze-dried and spray-dried bromelain from pineapple stems
publisher Science Faculty of Chiang Mai University
publishDate 2019
url http://it.science.cmu.ac.th/ejournal/dl.php?journal_id=8468
http://cmuir.cmu.ac.th/jspui/handle/6653943832/63964
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