A Simple High-performance Liquid Chromatography Coupled to Fluorescence Detection Method using Column-switching Technique for Measuring Urinary 1-hydroxypyrene from Environmental Exposure

• المؤلفون الرئيسيون: Kunrunya Sutan, Warangakana Naksen, Tippawan Prapamontol
• التنسيق: บทความวารสาร
• اللغة: English
• منشور في: Science Faculty of Chiang Mai University 2019
• الوصول للمادة أونلاين: http://it.science.cmu.ac.th/ejournal/dl.php?journal_id=8471; http://cmuir.cmu.ac.th/jspui/handle/6653943832/63971

A method for determination of urinary 1-hydroxypyrene (1-OHP) was developed using HPLC-FLD with a column-switching system. 1-OHP in urine samples was extracted by b-glucuronidase hydrolysis, followed by C18 SPE with optimized conditions. LOD and LOQ were 0.01 and 0.03 ng/mL, respectively; this was s...