Simultaneous Saccharification and Fermentation of L-(+)-Lactic Acid Production from Liquefied Cassava Starch by Immobilized Rhizopus oryzae in a 3 L Airlift Fermenter
L-(+)-lactic acid production by fermentation of starchy material (low-cost agricultural products) leads to a reduction in production cost and added value of agricultural products. Rhizopus oryzae DMKU 12 was selected because it can produce the highest amount of L-(+)-lactic acid from a-amylase-treat...
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| Main Authors: | , , |
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| Format: | บทความวารสาร |
| Language: | English |
| Published: |
Science Faculty of Chiang Mai University
2019
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| Online Access: | http://it.science.cmu.ac.th/ejournal/dl.php?journal_id=8731 http://cmuir.cmu.ac.th/jspui/handle/6653943832/64009 |
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| Institution: | Chiang Mai University |
| Language: | English |
| Summary: | L-(+)-lactic acid production by fermentation of starchy material (low-cost agricultural products) leads to a reduction in production cost and added value of agricultural products. Rhizopus oryzae DMKU 12 was selected because it can produce the highest amount of L-(+)-lactic acid from a-amylase-treated liquefied cassava starch. L-(+)-lactic acid production by R. oryzae DMKU 12 in a shaking flask indicated that KH2PO4 and ZnSO4.7H2O significantly affected L-(+)-lactic acid production; 150 g/L of a-amylase-treated liquefied cassava starch was the concentration that maximized production. L-(+)-lactic acid of 83.7 g/L with productivity of 1.16 g/L/h was obtained by R. oryzae DMKU 12 immobilized on a 0.25 ´ 0.25 ´ 0.25 cm3 polyurethane sponge at an aeration rate of 0.75 vvm after 72 h of cultivation in a 3 L airlift fermenter. Repeated-batch culture by PUS-immobilized R. oryzae was successful at 4 cycles, with average L-(+)-lactic acid concentration and product yield of 77.7 g/L and 0.62, respectively. |
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