Improvements in Activity and Stability of Immobilized Alcohol Dehydrogenase using Magnetic Carbon Nanotubes

Improvements in Activity and Stability of Immobilized Alcohol Dehydrogenase using Magnetic Carbon Nanotubes

Carbon nanotube (CNT) has been used for the immobilization of proteins and enzymes. However, the leakage of CNT into solution and difficulty in separation hindered the application of CNT. To minimize these shortcomings, CNT functionalized with magnetic particles was prepared and intended to be used...

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Main Authors: Xiaosheng Tang, Ping Tang, Liangliang Liu
Format: บทความวารสาร
Language:English
Published: Science Faculty of Chiang Mai University 2019
Online Access:http://it.science.cmu.ac.th/ejournal/dl.php?journal_id=8743
http://cmuir.cmu.ac.th/jspui/handle/6653943832/64019
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Institution: Chiang Mai University
Language: English
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spelling th-cmuir.6653943832-640192019-05-07T09:59:43Z Improvements in Activity and Stability of Immobilized Alcohol Dehydrogenase using Magnetic Carbon Nanotubes Xiaosheng Tang Ping Tang Liangliang Liu Carbon nanotube (CNT) has been used for the immobilization of proteins and enzymes. However, the leakage of CNT into solution and difficulty in separation hindered the application of CNT. To minimize these shortcomings, CNT functionalized with magnetic particles was prepared and intended to be used in the immobilization of alcohol dehydrogenase (ADH) in this study. The obtained magnetic CNT (MCNT) was characterized by Fourier transform infrared spectroscopy (FTIR), transmission electron microscopy (TEM), scanning electron microscopy (SEM) and vibration sample magnetometer (VSM). The operational stability was evaluated in repeated assay process, and the MCNT immobilized ADH (MCNT-ADH) retained 87.6 % of the initial activity after being used for 10 times. Compared with free ADH, MCNT-ADH exhibited improved pH and thermal stabilities. As a kind of novel immobilization support, MCNT could find considerable potential uses in industrial processes due to its better performance. 2019-05-07T09:59:43Z 2019-05-07T09:59:43Z 2018 บทความวารสาร 0125-2526 http://it.science.cmu.ac.th/ejournal/dl.php?journal_id=8743 http://cmuir.cmu.ac.th/jspui/handle/6653943832/64019 Eng Science Faculty of Chiang Mai University
institution Chiang Mai University
building Chiang Mai University Library
country Thailand
collection CMU Intellectual Repository
language English
description Carbon nanotube (CNT) has been used for the immobilization of proteins and enzymes. However, the leakage of CNT into solution and difficulty in separation hindered the application of CNT. To minimize these shortcomings, CNT functionalized with magnetic particles was prepared and intended to be used in the immobilization of alcohol dehydrogenase (ADH) in this study. The obtained magnetic CNT (MCNT) was characterized by Fourier transform infrared spectroscopy (FTIR), transmission electron microscopy (TEM), scanning electron microscopy (SEM) and vibration sample magnetometer (VSM). The operational stability was evaluated in repeated assay process, and the MCNT immobilized ADH (MCNT-ADH) retained 87.6 % of the initial activity after being used for 10 times. Compared with free ADH, MCNT-ADH exhibited improved pH and thermal stabilities. As a kind of novel immobilization support, MCNT could find considerable potential uses in industrial processes due to its better performance.
format บทความวารสาร
author Xiaosheng Tang
Ping Tang
Liangliang Liu
spellingShingle Xiaosheng Tang
Ping Tang
Liangliang Liu
Improvements in Activity and Stability of Immobilized Alcohol Dehydrogenase using Magnetic Carbon Nanotubes
author_facet Xiaosheng Tang
Ping Tang
Liangliang Liu
author_sort Xiaosheng Tang
title Improvements in Activity and Stability of Immobilized Alcohol Dehydrogenase using Magnetic Carbon Nanotubes
title_short Improvements in Activity and Stability of Immobilized Alcohol Dehydrogenase using Magnetic Carbon Nanotubes
title_full Improvements in Activity and Stability of Immobilized Alcohol Dehydrogenase using Magnetic Carbon Nanotubes
title_fullStr Improvements in Activity and Stability of Immobilized Alcohol Dehydrogenase using Magnetic Carbon Nanotubes
title_full_unstemmed Improvements in Activity and Stability of Immobilized Alcohol Dehydrogenase using Magnetic Carbon Nanotubes
title_sort improvements in activity and stability of immobilized alcohol dehydrogenase using magnetic carbon nanotubes
publisher Science Faculty of Chiang Mai University
publishDate 2019
url http://it.science.cmu.ac.th/ejournal/dl.php?journal_id=8743
http://cmuir.cmu.ac.th/jspui/handle/6653943832/64019
_version_ 1681426003481591808

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