Caffeoylquinic Acids Induce Cell Death and Cell Cycle Arrest on HCT 116 Cells via Formation of Extracellular H2O2 and Quinones

Caffeoylquinic Acids Induce Cell Death and Cell Cycle Arrest on HCT 116 Cells via Formation of Extracellular H2O2 and Quinones

Caffeoylquinic acids are frequently present in variety of medicinal plants, fruits, vegetables and beverages. These phenolic acids are widely accepted as antioxidants but have pro-oxidant activity under certain condition. The cell death and cell cycle arrest on HCT 116 human colon carcinoma cells we...

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Main Authors: Wuen Yew Teoh, Norhanom Abdul Wahab, Kae Shin Sim
Format: บทความวารสาร
Language:English
Published: Science Faculty of Chiang Mai University 2019
Online Access:http://it.science.cmu.ac.th/ejournal/dl.php?journal_id=8752
http://cmuir.cmu.ac.th/jspui/handle/6653943832/64034
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Institution: Chiang Mai University
Language: English
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spelling th-cmuir.6653943832-640342019-05-07T09:59:44Z Caffeoylquinic Acids Induce Cell Death and Cell Cycle Arrest on HCT 116 Cells via Formation of Extracellular H2O2 and Quinones Wuen Yew Teoh Norhanom Abdul Wahab Kae Shin Sim Caffeoylquinic acids are frequently present in variety of medicinal plants, fruits, vegetables and beverages. These phenolic acids are widely accepted as antioxidants but have pro-oxidant activity under certain condition. The cell death and cell cycle arrest on HCT 116 human colon carcinoma cells were observed by flow cytometry when treated with 5-O-caffeoylquinic acid (CQ) and 3,5-dicaffeoylquinic acid (DCQ) at 1 and 2 mg/ml. The addition of cell impermeable catalase and reduced glutathione (GSH) could protect HCT 116 cells from cell death and cell cycle arrest effect of CQ and DCQ. It was observed that CQ and DCQ generated extracellular hydrogen peroxide (H2O2) and quinones in the culture medium. These in vitro data showed that caffeoylquinic acids have to undergo oxidative mechanism in order to induce anti-proliferation effect on colon cancer cells and this may be beneficial in prevention or treatment of colorectal cancer. 2019-05-07T09:59:44Z 2019-05-07T09:59:44Z 2018 บทความวารสาร 0125-2526 http://it.science.cmu.ac.th/ejournal/dl.php?journal_id=8752 http://cmuir.cmu.ac.th/jspui/handle/6653943832/64034 Eng Science Faculty of Chiang Mai University
institution Chiang Mai University
building Chiang Mai University Library
country Thailand
collection CMU Intellectual Repository
language English
description Caffeoylquinic acids are frequently present in variety of medicinal plants, fruits, vegetables and beverages. These phenolic acids are widely accepted as antioxidants but have pro-oxidant activity under certain condition. The cell death and cell cycle arrest on HCT 116 human colon carcinoma cells were observed by flow cytometry when treated with 5-O-caffeoylquinic acid (CQ) and 3,5-dicaffeoylquinic acid (DCQ) at 1 and 2 mg/ml. The addition of cell impermeable catalase and reduced glutathione (GSH) could protect HCT 116 cells from cell death and cell cycle arrest effect of CQ and DCQ. It was observed that CQ and DCQ generated extracellular hydrogen peroxide (H2O2) and quinones in the culture medium. These in vitro data showed that caffeoylquinic acids have to undergo oxidative mechanism in order to induce anti-proliferation effect on colon cancer cells and this may be beneficial in prevention or treatment of colorectal cancer.
format บทความวารสาร
author Wuen Yew Teoh
Norhanom Abdul Wahab
Kae Shin Sim
spellingShingle Wuen Yew Teoh
Norhanom Abdul Wahab
Kae Shin Sim
Caffeoylquinic Acids Induce Cell Death and Cell Cycle Arrest on HCT 116 Cells via Formation of Extracellular H2O2 and Quinones
author_facet Wuen Yew Teoh
Norhanom Abdul Wahab
Kae Shin Sim
author_sort Wuen Yew Teoh
title Caffeoylquinic Acids Induce Cell Death and Cell Cycle Arrest on HCT 116 Cells via Formation of Extracellular H2O2 and Quinones
title_short Caffeoylquinic Acids Induce Cell Death and Cell Cycle Arrest on HCT 116 Cells via Formation of Extracellular H2O2 and Quinones
title_full Caffeoylquinic Acids Induce Cell Death and Cell Cycle Arrest on HCT 116 Cells via Formation of Extracellular H2O2 and Quinones
title_fullStr Caffeoylquinic Acids Induce Cell Death and Cell Cycle Arrest on HCT 116 Cells via Formation of Extracellular H2O2 and Quinones
title_full_unstemmed Caffeoylquinic Acids Induce Cell Death and Cell Cycle Arrest on HCT 116 Cells via Formation of Extracellular H2O2 and Quinones
title_sort caffeoylquinic acids induce cell death and cell cycle arrest on hct 116 cells via formation of extracellular h2o2 and quinones
publisher Science Faculty of Chiang Mai University
publishDate 2019
url http://it.science.cmu.ac.th/ejournal/dl.php?journal_id=8752
http://cmuir.cmu.ac.th/jspui/handle/6653943832/64034
_version_ 1681426006289678336

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