Screening of Antagonistic Bacterial Isolates from Hives of Apis cerana in Vietnam Against the Causal Agent of American Foulbrood of Honey Bees, Paenibacillus larvae
American foulbrood (AFB) is a virulent disease of honey bee brood caused by the Gram-positive, spore-forming bacterium; Paenibacillus larvae. In this study, we determined the potential of bacteria isolated from hives of Asian honey bees (Apis cerana) to act antagonistically against P. larvae. Isolat...
Saved in:
Main Authors: | , , , |
---|---|
Format: | บทความวารสาร |
Language: | English |
Published: |
Science Faculty of Chiang Mai University
2019
|
Online Access: | http://it.science.cmu.ac.th/ejournal/dl.php?journal_id=9130 http://cmuir.cmu.ac.th/jspui/handle/6653943832/64105 |
Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
Institution: | Chiang Mai University |
Language: | English |
id |
th-cmuir.6653943832-64105 |
---|---|
record_format |
dspace |
spelling |
th-cmuir.6653943832-641052019-05-07T09:59:47Z Screening of Antagonistic Bacterial Isolates from Hives of Apis cerana in Vietnam Against the Causal Agent of American Foulbrood of Honey Bees, Paenibacillus larvae Sasiprapa Krongdang Jeffery S. Pettis Geoffrey R. Williams Panuwan Chantawannakul American foulbrood (AFB) is a virulent disease of honey bee brood caused by the Gram-positive, spore-forming bacterium; Paenibacillus larvae. In this study, we determined the potential of bacteria isolated from hives of Asian honey bees (Apis cerana) to act antagonistically against P. larvae. Isolates were sampled from different locations on the fronts of A. cerana hives in Vietnam. A total of 69 isolates were obtained through a culture-dependent method and 16S rRNA gene sequencing showed affiliation to the phyla Firmicutes and Actinobacteria. Out of 69 isolates, 15 showed strong inhibitory activity against P. larvae; Bacillus pocheonensis (VN101) showed the largest zone of inhibition (26 ฑ 1 mm). In this study, the diversity and richness of antagonistic isolates indicated that Bacillus spp. are the most promising as inhibitors of P. larvae. These finding suggested that certain bacterial isolates can act as antagonist to control P. larvae and may have other biotechnological applications. 2019-05-07T09:59:47Z 2019-05-07T09:59:47Z 2018 บทความวารสาร 0125-2526 http://it.science.cmu.ac.th/ejournal/dl.php?journal_id=9130 http://cmuir.cmu.ac.th/jspui/handle/6653943832/64105 Eng Science Faculty of Chiang Mai University |
institution |
Chiang Mai University |
building |
Chiang Mai University Library |
country |
Thailand |
collection |
CMU Intellectual Repository |
language |
English |
description |
American foulbrood (AFB) is a virulent disease of honey bee brood caused by the Gram-positive, spore-forming bacterium; Paenibacillus larvae. In this study, we determined the potential of bacteria isolated from hives of Asian honey bees (Apis cerana) to act antagonistically against P. larvae. Isolates were sampled from different locations on the fronts of A. cerana hives in Vietnam. A total of 69 isolates were obtained through a culture-dependent method and 16S rRNA gene sequencing showed affiliation to the phyla Firmicutes and Actinobacteria. Out of 69 isolates, 15 showed strong inhibitory activity against P. larvae; Bacillus pocheonensis (VN101) showed the largest zone of inhibition (26 ฑ 1 mm). In this study, the diversity and richness of antagonistic isolates indicated that Bacillus spp. are the most promising as inhibitors of P. larvae. These finding suggested that certain bacterial isolates can act as antagonist to control P. larvae and may have other biotechnological applications. |
format |
บทความวารสาร |
author |
Sasiprapa Krongdang Jeffery S. Pettis Geoffrey R. Williams Panuwan Chantawannakul |
spellingShingle |
Sasiprapa Krongdang Jeffery S. Pettis Geoffrey R. Williams Panuwan Chantawannakul Screening of Antagonistic Bacterial Isolates from Hives of Apis cerana in Vietnam Against the Causal Agent of American Foulbrood of Honey Bees, Paenibacillus larvae |
author_facet |
Sasiprapa Krongdang Jeffery S. Pettis Geoffrey R. Williams Panuwan Chantawannakul |
author_sort |
Sasiprapa Krongdang |
title |
Screening of Antagonistic Bacterial Isolates from Hives of Apis cerana in Vietnam Against the Causal Agent of American Foulbrood of Honey Bees, Paenibacillus larvae |
title_short |
Screening of Antagonistic Bacterial Isolates from Hives of Apis cerana in Vietnam Against the Causal Agent of American Foulbrood of Honey Bees, Paenibacillus larvae |
title_full |
Screening of Antagonistic Bacterial Isolates from Hives of Apis cerana in Vietnam Against the Causal Agent of American Foulbrood of Honey Bees, Paenibacillus larvae |
title_fullStr |
Screening of Antagonistic Bacterial Isolates from Hives of Apis cerana in Vietnam Against the Causal Agent of American Foulbrood of Honey Bees, Paenibacillus larvae |
title_full_unstemmed |
Screening of Antagonistic Bacterial Isolates from Hives of Apis cerana in Vietnam Against the Causal Agent of American Foulbrood of Honey Bees, Paenibacillus larvae |
title_sort |
screening of antagonistic bacterial isolates from hives of apis cerana in vietnam against the causal agent of american foulbrood of honey bees, paenibacillus larvae |
publisher |
Science Faculty of Chiang Mai University |
publishDate |
2019 |
url |
http://it.science.cmu.ac.th/ejournal/dl.php?journal_id=9130 http://cmuir.cmu.ac.th/jspui/handle/6653943832/64105 |
_version_ |
1681426019542630400 |