Comparative GC-MS Analysis, In-vitro Antioxidant and Antimicrobial Activities of the Essential Oils Isolated from the Peel of Omani Lime
The aim of this study was, to analyze and compare the composition of volatile constituents in the essential oils from the peel of Citrus aurantifolia L. grown in three different regions of Oman and to investigate their antioxidant and antibacterial potential. The essential oil from the fresh peels w...
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Science Faculty of Chiang Mai University
2019
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th-cmuir.6653943832-641542019-05-07T09:59:50Z Comparative GC-MS Analysis, In-vitro Antioxidant and Antimicrobial Activities of the Essential Oils Isolated from the Peel of Omani Lime Amwaj Mohammed Al-Breiki Huda Mubarak Al-Brashdi Jamal Nasser Al-Sabahi Shah Alam Khan The aim of this study was, to analyze and compare the composition of volatile constituents in the essential oils from the peel of Citrus aurantifolia L. grown in three different regions of Oman and to investigate their antioxidant and antibacterial potential. The essential oil from the fresh peels was isolated by hydro-distillation method and chemical composition analysis of oils was carried out by Gas chromatography–Mass spectrometry (GC-MS). The antioxidant activity was investigated by a non enzymatic free radical scavenging method while antibacterial activity was tested against gram positive and gram negative bacteria by disc diffusion method. Molecular docking studies were also carried out to probe the mechanism of antioxidant activity. GC-MS analysis indicated variation in content and composition of chemical constituents in the peel oils. Limonene was found to be the major volatile constituent thus establishing lime peel oil as limonene chemotype. The peel essential oils of C. aurantifolia showed moderate antioxidant and antibacterial activitities. Molecular docking studies indicated α-farnesene to have better binding affinity for Xanthine oxidoreductase in comparison to limonene and pinene. Further studies are needed to develop the lime peel oil as an antimicrobial preservative and an alternative source of natural antioxidant(s). 2019-05-07T09:59:50Z 2019-05-07T09:59:50Z 2018 บทความวารสาร 0125-2526 http://it.science.cmu.ac.th/ejournal/dl.php?journal_id=9318 http://cmuir.cmu.ac.th/jspui/handle/6653943832/64154 Eng Science Faculty of Chiang Mai University |
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The aim of this study was, to analyze and compare the composition of volatile constituents in the essential oils from the peel of Citrus aurantifolia L. grown in three different regions of Oman and to investigate their antioxidant and antibacterial potential. The essential oil from the fresh peels was isolated by hydro-distillation method and chemical composition analysis of oils was carried out by Gas chromatography–Mass spectrometry (GC-MS). The antioxidant activity was investigated by a non enzymatic free radical scavenging method while antibacterial activity was tested against gram positive and gram negative bacteria by disc diffusion method. Molecular docking studies were also carried out to probe the mechanism of antioxidant activity. GC-MS analysis indicated variation in content and composition of chemical constituents in the peel oils. Limonene was found to be the major volatile constituent thus establishing lime peel oil as limonene chemotype. The peel essential oils of C. aurantifolia showed moderate antioxidant and antibacterial activitities. Molecular docking studies indicated α-farnesene to have better binding affinity for Xanthine oxidoreductase in comparison to limonene and pinene. Further studies are needed to develop the lime peel oil as an antimicrobial preservative and an alternative source of natural antioxidant(s). |
format |
บทความวารสาร |
author |
Amwaj Mohammed Al-Breiki Huda Mubarak Al-Brashdi Jamal Nasser Al-Sabahi Shah Alam Khan |
spellingShingle |
Amwaj Mohammed Al-Breiki Huda Mubarak Al-Brashdi Jamal Nasser Al-Sabahi Shah Alam Khan Comparative GC-MS Analysis, In-vitro Antioxidant and Antimicrobial Activities of the Essential Oils Isolated from the Peel of Omani Lime |
author_facet |
Amwaj Mohammed Al-Breiki Huda Mubarak Al-Brashdi Jamal Nasser Al-Sabahi Shah Alam Khan |
author_sort |
Amwaj Mohammed Al-Breiki |
title |
Comparative GC-MS Analysis, In-vitro Antioxidant and Antimicrobial Activities of the Essential Oils Isolated from the Peel of Omani Lime |
title_short |
Comparative GC-MS Analysis, In-vitro Antioxidant and Antimicrobial Activities of the Essential Oils Isolated from the Peel of Omani Lime |
title_full |
Comparative GC-MS Analysis, In-vitro Antioxidant and Antimicrobial Activities of the Essential Oils Isolated from the Peel of Omani Lime |
title_fullStr |
Comparative GC-MS Analysis, In-vitro Antioxidant and Antimicrobial Activities of the Essential Oils Isolated from the Peel of Omani Lime |
title_full_unstemmed |
Comparative GC-MS Analysis, In-vitro Antioxidant and Antimicrobial Activities of the Essential Oils Isolated from the Peel of Omani Lime |
title_sort |
comparative gc-ms analysis, in-vitro antioxidant and antimicrobial activities of the essential oils isolated from the peel of omani lime |
publisher |
Science Faculty of Chiang Mai University |
publishDate |
2019 |
url |
http://it.science.cmu.ac.th/ejournal/dl.php?journal_id=9318 http://cmuir.cmu.ac.th/jspui/handle/6653943832/64154 |
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1681426028702990336 |