Pencil Graphite Based Biosensor for Rapid Malathion Pesticide Detection
This research described a sensitive deposable electrochemical biosensor for the detection of organophosphate pesticide based on acetylcholinesterase enzyme (AChE). The pencil graphite electrode was applied as working electrode to modify a disposable biosensor. A 0.5 U mg-1 AChE and 0.25 %wt Chitosan...
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| Main Author: | |
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| Format: | บทความวารสาร |
| Language: | English |
| Published: |
Science Faculty of Chiang Mai University
2019
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| Online Access: | http://it.science.cmu.ac.th/ejournal/dl.php?journal_id=9523 http://cmuir.cmu.ac.th/jspui/handle/6653943832/64212 |
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| Institution: | Chiang Mai University |
| Language: | English |
| Summary: | This research described a sensitive deposable electrochemical biosensor for the detection of organophosphate pesticide based on acetylcholinesterase enzyme (AChE). The pencil graphite electrode was applied as working electrode to modify a disposable biosensor. A 0.5 U mg-1 AChE and 0.25 %wt Chitosan (Chit) were modified as enzyme and conductive polymer to produce PGE/PB/AChE/Chit biosensor by dropping method. Malathion was applied as a pesticide sample in this research. The detection conditions were employed under potential 0.3 V in 0.1 M phosphate buffer solution (pH 7.0) containing 15 mM acetylcholine chloride by chronoamperometry (CA). The PGE/PB/AChE/Chit biosensor exhibited detection limits at 3.0607 ng ml-1. The linear range, sensitivity, response time, reproducibility are 0.5-16.5 ngml-1, 0.8867 mA nM-1, 50 s, 4.43% RSD, respectively. The storage performance at 35 days were 77.97% remained. The proposed biosensor was successfully applied in the determination of malathion pesticide sample. |
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