Characterization, Antifungal Activity and Plant Growth Promoting Potential of Endophytic Actinomycetes Isolated from Rice (Oryza sativa L.)

Actinomycetes are well known as producers of biologically active compounds with high commercial value. They have been isolated from various sources including plant species. In this study, the plant growth promoting traits of endophytic actinomycetes isolated from rice plants were evaluated, as well...

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Main Authors: Ratchanee Mingma, Kannika Duangmal
Format: บทความวารสาร
Language:English
Published: Science Faculty of Chiang Mai University 2019
Online Access:http://it.science.cmu.ac.th/ejournal/dl.php?journal_id=9658
http://cmuir.cmu.ac.th/jspui/handle/6653943832/64234
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Institution: Chiang Mai University
Language: English
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spelling th-cmuir.6653943832-642342019-05-07T09:59:54Z Characterization, Antifungal Activity and Plant Growth Promoting Potential of Endophytic Actinomycetes Isolated from Rice (Oryza sativa L.) Ratchanee Mingma Kannika Duangmal Actinomycetes are well known as producers of biologically active compounds with high commercial value. They have been isolated from various sources including plant species. In this study, the plant growth promoting traits of endophytic actinomycetes isolated from rice plants were evaluated, as well as their antagonistic activities against rice pathogenic fungi. Healthy rice plants were collected from nine provinces in Thailand. One hundred and ninety-one isolates were able to be recovered from the surface-sterilized roots and stems of the rice plants. Of these, 96 actinomycetes were classified as streptomycetes and 95 strains were non-streptomycetes species. Plant growth promotion assays showed that 79.6% and 30.9% of these isolates could produce siderophore and indole-3-acetic acid, respectively and 42.9% could solubilize phosphate. Potential strains which showed strongly antagonistic against rice pathogenic fungi, Fusarium oxysporum, Rhizoctonia solani and Helminthosporium oryzae, with plant growth promoting activities were selected to determine their effects on root system of rice seedlings in vitro. The results showed that the isolates R07-04, R07-06 and S03-26 could increase the number of adventitious roots, but led to shorter seminal roots. However, in the pot experiment, isolate R07-06 could promote rice growth better than the other isolates and significantly enhanced shoot length, root dry weight and shoot dry weight. An identification of the isolate R07-06 using the 16S rRNA gene showed that the strain was most closely related to Streptomyces lydicus DSM 40002T (100% similarity). These results demonstrate that endophytic Streptomyces R07-06 could be a promising strain to develop as an antifungal and plant growth promoting agent for rice plants. 2019-05-07T09:59:53Z 2019-05-07T09:59:53Z 2018 บทความวารสาร 0125-2526 http://it.science.cmu.ac.th/ejournal/dl.php?journal_id=9658 http://cmuir.cmu.ac.th/jspui/handle/6653943832/64234 Eng Science Faculty of Chiang Mai University
institution Chiang Mai University
building Chiang Mai University Library
country Thailand
collection CMU Intellectual Repository
language English
description Actinomycetes are well known as producers of biologically active compounds with high commercial value. They have been isolated from various sources including plant species. In this study, the plant growth promoting traits of endophytic actinomycetes isolated from rice plants were evaluated, as well as their antagonistic activities against rice pathogenic fungi. Healthy rice plants were collected from nine provinces in Thailand. One hundred and ninety-one isolates were able to be recovered from the surface-sterilized roots and stems of the rice plants. Of these, 96 actinomycetes were classified as streptomycetes and 95 strains were non-streptomycetes species. Plant growth promotion assays showed that 79.6% and 30.9% of these isolates could produce siderophore and indole-3-acetic acid, respectively and 42.9% could solubilize phosphate. Potential strains which showed strongly antagonistic against rice pathogenic fungi, Fusarium oxysporum, Rhizoctonia solani and Helminthosporium oryzae, with plant growth promoting activities were selected to determine their effects on root system of rice seedlings in vitro. The results showed that the isolates R07-04, R07-06 and S03-26 could increase the number of adventitious roots, but led to shorter seminal roots. However, in the pot experiment, isolate R07-06 could promote rice growth better than the other isolates and significantly enhanced shoot length, root dry weight and shoot dry weight. An identification of the isolate R07-06 using the 16S rRNA gene showed that the strain was most closely related to Streptomyces lydicus DSM 40002T (100% similarity). These results demonstrate that endophytic Streptomyces R07-06 could be a promising strain to develop as an antifungal and plant growth promoting agent for rice plants.
format บทความวารสาร
author Ratchanee Mingma
Kannika Duangmal
spellingShingle Ratchanee Mingma
Kannika Duangmal
Characterization, Antifungal Activity and Plant Growth Promoting Potential of Endophytic Actinomycetes Isolated from Rice (Oryza sativa L.)
author_facet Ratchanee Mingma
Kannika Duangmal
author_sort Ratchanee Mingma
title Characterization, Antifungal Activity and Plant Growth Promoting Potential of Endophytic Actinomycetes Isolated from Rice (Oryza sativa L.)
title_short Characterization, Antifungal Activity and Plant Growth Promoting Potential of Endophytic Actinomycetes Isolated from Rice (Oryza sativa L.)
title_full Characterization, Antifungal Activity and Plant Growth Promoting Potential of Endophytic Actinomycetes Isolated from Rice (Oryza sativa L.)
title_fullStr Characterization, Antifungal Activity and Plant Growth Promoting Potential of Endophytic Actinomycetes Isolated from Rice (Oryza sativa L.)
title_full_unstemmed Characterization, Antifungal Activity and Plant Growth Promoting Potential of Endophytic Actinomycetes Isolated from Rice (Oryza sativa L.)
title_sort characterization, antifungal activity and plant growth promoting potential of endophytic actinomycetes isolated from rice (oryza sativa l.)
publisher Science Faculty of Chiang Mai University
publishDate 2019
url http://it.science.cmu.ac.th/ejournal/dl.php?journal_id=9658
http://cmuir.cmu.ac.th/jspui/handle/6653943832/64234
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