Interaction of CD99 and its ligand upregulates IL-6 and TNF-α upon T cell activation

© 2019 Takheaw et al. This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. CD99 has been reported to be involved in T...

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Main Authors: Nuchjira Takheaw, Papawadee Earwong, Witida Laopajon, Supansa Pata, Watchara Kasinrerk
Format: Journal
Published: 2019
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http://cmuir.cmu.ac.th/jspui/handle/6653943832/65264
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Institution: Chiang Mai University
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spelling th-cmuir.6653943832-652642020-04-02T15:21:19Z Interaction of CD99 and its ligand upregulates IL-6 and TNF-α upon T cell activation Nuchjira Takheaw Papawadee Earwong Witida Laopajon Supansa Pata Watchara Kasinrerk Agricultural and Biological Sciences Biochemistry, Genetics and Molecular Biology Multidisciplinary © 2019 Takheaw et al. This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. CD99 has been reported to be involved in T cell regulation. CD99 ligand involvement in the regulation of T cell activation has been postulated. In this study, recombinant CD99 proteins were produced and used as a tool for determining the role of CD99 and its ligand interaction. Recombinant CD99 proteins induced the upregulation of IL-6 and TNF-α expression, but not IFN-γ, in anti-CD3 monoclonal antibody activated T cells. The cytokine alteration was not observed in unstimulated T cells indicating the cytokine upregulation required the signal from T cell activation. The upregulation of IL-6 and TNF-α was, in addition, observed in CD3-mononuclear cell population including monocytes and NK cells. The recombinant CD99 proteins, however, did not affect either CD25, CD69 or MHC class II expression or T cell proliferation, upon T cell activation. The CD99 ligands were demonstrated to be expressed on monocytes, NK cells and dendritic cells, but not on B and T cells. Our results indicated the presence of CD99 ligands on leukocyte surface. Interaction between CD99 and its ligands involves the regulation of cytokine production. 2019-08-05T04:31:05Z 2019-08-05T04:31:05Z 2019-05-01 Journal 19326203 2-s2.0-85066821898 10.1371/journal.pone.0217393 https://www.scopus.com/inward/record.uri?partnerID=HzOxMe3b&scp=85066821898&origin=inward http://cmuir.cmu.ac.th/jspui/handle/6653943832/65264
institution Chiang Mai University
building Chiang Mai University Library
country Thailand
collection CMU Intellectual Repository
topic Agricultural and Biological Sciences
Biochemistry, Genetics and Molecular Biology
Multidisciplinary
spellingShingle Agricultural and Biological Sciences
Biochemistry, Genetics and Molecular Biology
Multidisciplinary
Nuchjira Takheaw
Papawadee Earwong
Witida Laopajon
Supansa Pata
Watchara Kasinrerk
Interaction of CD99 and its ligand upregulates IL-6 and TNF-α upon T cell activation
description © 2019 Takheaw et al. This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. CD99 has been reported to be involved in T cell regulation. CD99 ligand involvement in the regulation of T cell activation has been postulated. In this study, recombinant CD99 proteins were produced and used as a tool for determining the role of CD99 and its ligand interaction. Recombinant CD99 proteins induced the upregulation of IL-6 and TNF-α expression, but not IFN-γ, in anti-CD3 monoclonal antibody activated T cells. The cytokine alteration was not observed in unstimulated T cells indicating the cytokine upregulation required the signal from T cell activation. The upregulation of IL-6 and TNF-α was, in addition, observed in CD3-mononuclear cell population including monocytes and NK cells. The recombinant CD99 proteins, however, did not affect either CD25, CD69 or MHC class II expression or T cell proliferation, upon T cell activation. The CD99 ligands were demonstrated to be expressed on monocytes, NK cells and dendritic cells, but not on B and T cells. Our results indicated the presence of CD99 ligands on leukocyte surface. Interaction between CD99 and its ligands involves the regulation of cytokine production.
format Journal
author Nuchjira Takheaw
Papawadee Earwong
Witida Laopajon
Supansa Pata
Watchara Kasinrerk
author_facet Nuchjira Takheaw
Papawadee Earwong
Witida Laopajon
Supansa Pata
Watchara Kasinrerk
author_sort Nuchjira Takheaw
title Interaction of CD99 and its ligand upregulates IL-6 and TNF-α upon T cell activation
title_short Interaction of CD99 and its ligand upregulates IL-6 and TNF-α upon T cell activation
title_full Interaction of CD99 and its ligand upregulates IL-6 and TNF-α upon T cell activation
title_fullStr Interaction of CD99 and its ligand upregulates IL-6 and TNF-α upon T cell activation
title_full_unstemmed Interaction of CD99 and its ligand upregulates IL-6 and TNF-α upon T cell activation
title_sort interaction of cd99 and its ligand upregulates il-6 and tnf-α upon t cell activation
publishDate 2019
url https://www.scopus.com/inward/record.uri?partnerID=HzOxMe3b&scp=85066821898&origin=inward
http://cmuir.cmu.ac.th/jspui/handle/6653943832/65264
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