Ethanolic extract of Kaempferia parviflora interrupts the mechanisms-associated rheumatoid arthritis in SW982 culture model via p38/STAT1 and STAT3 pathways

© 2018 Elsevier GmbH Background: Kaempferia parviflora Wall. ex Baker (KP) has long been used in traditional medicine to treat various diseases because active compounds in rhizome extracts are important anti-inflammatory agents. Purpose: This study aims to investigate the effects of an ethanolic ext...

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Main Authors: Patiwat Kongdang, Rungnaree Jaitham, Supitcha Thonghoi, Chakkrapong Kuensaen, Waranee Pradit, Siriwan Ongchai
Format: Journal
Published: 2019
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http://cmuir.cmu.ac.th/jspui/handle/6653943832/65372
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spelling th-cmuir.6653943832-653722019-08-05T04:42:45Z Ethanolic extract of Kaempferia parviflora interrupts the mechanisms-associated rheumatoid arthritis in SW982 culture model via p38/STAT1 and STAT3 pathways Patiwat Kongdang Rungnaree Jaitham Supitcha Thonghoi Chakkrapong Kuensaen Waranee Pradit Siriwan Ongchai Biochemistry, Genetics and Molecular Biology Medicine Pharmacology, Toxicology and Pharmaceutics © 2018 Elsevier GmbH Background: Kaempferia parviflora Wall. ex Baker (KP) has long been used in traditional medicine to treat various diseases because active compounds in rhizome extracts are important anti-inflammatory agents. Purpose: This study aims to investigate the effects of an ethanolic extract of KP on the molecular mechanisms associated with rheumatoid arthritis (RA), which was induced by a combination of proinflammatory cytokines (IL-1β or TNF-α with IL-17A) in a human synovial sarcoma cell line (SW982) culture model. Methods: SW982 cells pretreated with cytokines were incubated with KP extract at 3–30 µg/ml, or three major compounds of KP (5,7-dimethoxyflavone, 5,7,4′-trimethoxyflavone, and 3,5,7,3′,4′-pentamethoxyflavone) for up to 72 h. Dexamethasone was used as positive control. RA-associated genes and inflammatory products were measured in parallel with cell death genes. Apoptosis by flow cytometry and migration assay were also analyzed. Western blotting was used to examine the effects on intracellular signaling mechanisms. Results: KP extract markedly reduced the expression of genes and levels of proinflammatory cytokines, inflammatory mediators, and matrix-degraded enzymes, but neither induced apoptosis nor altered the cell cycle. Its major constituents differently exerted suppressive effects on inflammatory genes. The KP extract downregulated the expression of genes associated with autophagosome and necroptosome formations. The extract also inhibited cell migration, reduced the mRNA expression of cadherin-11, and selectively reduced the phosphorylation of p38 MAPK, STAT1, and STAT3 signaling molecules, but did not interfere with the NF-κB pathway. Conclusion: These results suggest that the anti-arthritic potential of KP extract results from anti-inflammation and anti-migration via the suppression of the cytokines-induced p38/STAT1 and STAT3 pathways. 2019-08-05T04:32:16Z 2019-08-05T04:32:16Z 2019-06-01 Journal 1618095X 09447113 2-s2.0-85064312138 10.1016/j.phymed.2018.11.015 https://www.scopus.com/inward/record.uri?partnerID=HzOxMe3b&scp=85064312138&origin=inward http://cmuir.cmu.ac.th/jspui/handle/6653943832/65372
institution Chiang Mai University
building Chiang Mai University Library
country Thailand
collection CMU Intellectual Repository
topic Biochemistry, Genetics and Molecular Biology
Medicine
Pharmacology, Toxicology and Pharmaceutics
spellingShingle Biochemistry, Genetics and Molecular Biology
Medicine
Pharmacology, Toxicology and Pharmaceutics
Patiwat Kongdang
Rungnaree Jaitham
Supitcha Thonghoi
Chakkrapong Kuensaen
Waranee Pradit
Siriwan Ongchai
Ethanolic extract of Kaempferia parviflora interrupts the mechanisms-associated rheumatoid arthritis in SW982 culture model via p38/STAT1 and STAT3 pathways
description © 2018 Elsevier GmbH Background: Kaempferia parviflora Wall. ex Baker (KP) has long been used in traditional medicine to treat various diseases because active compounds in rhizome extracts are important anti-inflammatory agents. Purpose: This study aims to investigate the effects of an ethanolic extract of KP on the molecular mechanisms associated with rheumatoid arthritis (RA), which was induced by a combination of proinflammatory cytokines (IL-1β or TNF-α with IL-17A) in a human synovial sarcoma cell line (SW982) culture model. Methods: SW982 cells pretreated with cytokines were incubated with KP extract at 3–30 µg/ml, or three major compounds of KP (5,7-dimethoxyflavone, 5,7,4′-trimethoxyflavone, and 3,5,7,3′,4′-pentamethoxyflavone) for up to 72 h. Dexamethasone was used as positive control. RA-associated genes and inflammatory products were measured in parallel with cell death genes. Apoptosis by flow cytometry and migration assay were also analyzed. Western blotting was used to examine the effects on intracellular signaling mechanisms. Results: KP extract markedly reduced the expression of genes and levels of proinflammatory cytokines, inflammatory mediators, and matrix-degraded enzymes, but neither induced apoptosis nor altered the cell cycle. Its major constituents differently exerted suppressive effects on inflammatory genes. The KP extract downregulated the expression of genes associated with autophagosome and necroptosome formations. The extract also inhibited cell migration, reduced the mRNA expression of cadherin-11, and selectively reduced the phosphorylation of p38 MAPK, STAT1, and STAT3 signaling molecules, but did not interfere with the NF-κB pathway. Conclusion: These results suggest that the anti-arthritic potential of KP extract results from anti-inflammation and anti-migration via the suppression of the cytokines-induced p38/STAT1 and STAT3 pathways.
format Journal
author Patiwat Kongdang
Rungnaree Jaitham
Supitcha Thonghoi
Chakkrapong Kuensaen
Waranee Pradit
Siriwan Ongchai
author_facet Patiwat Kongdang
Rungnaree Jaitham
Supitcha Thonghoi
Chakkrapong Kuensaen
Waranee Pradit
Siriwan Ongchai
author_sort Patiwat Kongdang
title Ethanolic extract of Kaempferia parviflora interrupts the mechanisms-associated rheumatoid arthritis in SW982 culture model via p38/STAT1 and STAT3 pathways
title_short Ethanolic extract of Kaempferia parviflora interrupts the mechanisms-associated rheumatoid arthritis in SW982 culture model via p38/STAT1 and STAT3 pathways
title_full Ethanolic extract of Kaempferia parviflora interrupts the mechanisms-associated rheumatoid arthritis in SW982 culture model via p38/STAT1 and STAT3 pathways
title_fullStr Ethanolic extract of Kaempferia parviflora interrupts the mechanisms-associated rheumatoid arthritis in SW982 culture model via p38/STAT1 and STAT3 pathways
title_full_unstemmed Ethanolic extract of Kaempferia parviflora interrupts the mechanisms-associated rheumatoid arthritis in SW982 culture model via p38/STAT1 and STAT3 pathways
title_sort ethanolic extract of kaempferia parviflora interrupts the mechanisms-associated rheumatoid arthritis in sw982 culture model via p38/stat1 and stat3 pathways
publishDate 2019
url https://www.scopus.com/inward/record.uri?partnerID=HzOxMe3b&scp=85064312138&origin=inward
http://cmuir.cmu.ac.th/jspui/handle/6653943832/65372
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