Characterization and dye decolorization potential of two laccases from the marine-derived fungus Pestalotiopsis sp.

© 2019 by the authors. Licensee MDPI, Basel, Switzerland. Two laccase-encoding genes from the marine-derived fungus Pestalotiopsis sp. have been cloned in Aspergillus niger for heterologous production, and the recombinant enzymes have been characterized to study their physicochemical properties, the...

Full description

Saved in:
Bibliographic Details
Main Authors: Saowanee Wikee, Juliette Hatton, Annick Turbé-Doan, Yann Mathieu, Marianne Daou, Anne Lomascolo, Abhishek Kumar, Saisamorn Lumyong, Giuliano Sciara, Craig B. Faulds, Eric Record
Format: Journal
Published: 2019
Subjects:
Online Access:https://www.scopus.com/inward/record.uri?partnerID=HzOxMe3b&scp=85064965131&origin=inward
http://cmuir.cmu.ac.th/jspui/handle/6653943832/65386
Tags: Add Tag
No Tags, Be the first to tag this record!
Institution: Chiang Mai University
id th-cmuir.6653943832-65386
record_format dspace
spelling th-cmuir.6653943832-653862019-08-05T04:34:50Z Characterization and dye decolorization potential of two laccases from the marine-derived fungus Pestalotiopsis sp. Saowanee Wikee Juliette Hatton Annick Turbé-Doan Yann Mathieu Marianne Daou Anne Lomascolo Abhishek Kumar Saisamorn Lumyong Giuliano Sciara Craig B. Faulds Eric Record Biochemistry, Genetics and Molecular Biology Chemical Engineering Chemistry Computer Science © 2019 by the authors. Licensee MDPI, Basel, Switzerland. Two laccase-encoding genes from the marine-derived fungus Pestalotiopsis sp. have been cloned in Aspergillus niger for heterologous production, and the recombinant enzymes have been characterized to study their physicochemical properties, their ability to decolorize textile dyes for potential biotechnological applications, and their activity in the presence of sea salt. The optimal pH and temperature of PsLac1 and PsLac2 differed in relation to the substrates tested, and both enzymes were shown to be extremely stable at temperatures up to 50 °C, retaining 100% activity after 3 h at 50 °C. Both enzymes were stable between pH 4-6. Different substrate specificities were exhibited, and the lowest Km and highest catalytic efficiency values were obtained against syringaldazine and 2,6-dimethoxyphenol (DMP) for PsLac1 and PsLac2, respectively. The industrially important dyes-Acid Yellow, Bromo Cresol Purple, Nitrosulfonazo III, and Reactive Black 5-were more efficiently decolorized by PsLac1 in the presence of the redox mediator 1-hydroxybenzotriazole (HBT). Activities were compared in saline conditions, and PsLac2 seemed more adapted to the presence of sea salt than PsLac1. The overall surface charges of the predicted PsLac three-dimensional models showed large negatively charged surfaces for PsLac2, as found in proteins for marine organisms, and more balanced solvent exposed charges for PsLac1, as seen in proteins from terrestrial organisms. 2019-08-05T04:32:31Z 2019-08-05T04:32:31Z 2019-04-02 Journal 14220067 16616596 2-s2.0-85064965131 10.3390/ijms20081864 https://www.scopus.com/inward/record.uri?partnerID=HzOxMe3b&scp=85064965131&origin=inward http://cmuir.cmu.ac.th/jspui/handle/6653943832/65386
institution Chiang Mai University
building Chiang Mai University Library
country Thailand
collection CMU Intellectual Repository
topic Biochemistry, Genetics and Molecular Biology
Chemical Engineering
Chemistry
Computer Science
spellingShingle Biochemistry, Genetics and Molecular Biology
Chemical Engineering
Chemistry
Computer Science
Saowanee Wikee
Juliette Hatton
Annick Turbé-Doan
Yann Mathieu
Marianne Daou
Anne Lomascolo
Abhishek Kumar
Saisamorn Lumyong
Giuliano Sciara
Craig B. Faulds
Eric Record
Characterization and dye decolorization potential of two laccases from the marine-derived fungus Pestalotiopsis sp.
description © 2019 by the authors. Licensee MDPI, Basel, Switzerland. Two laccase-encoding genes from the marine-derived fungus Pestalotiopsis sp. have been cloned in Aspergillus niger for heterologous production, and the recombinant enzymes have been characterized to study their physicochemical properties, their ability to decolorize textile dyes for potential biotechnological applications, and their activity in the presence of sea salt. The optimal pH and temperature of PsLac1 and PsLac2 differed in relation to the substrates tested, and both enzymes were shown to be extremely stable at temperatures up to 50 °C, retaining 100% activity after 3 h at 50 °C. Both enzymes were stable between pH 4-6. Different substrate specificities were exhibited, and the lowest Km and highest catalytic efficiency values were obtained against syringaldazine and 2,6-dimethoxyphenol (DMP) for PsLac1 and PsLac2, respectively. The industrially important dyes-Acid Yellow, Bromo Cresol Purple, Nitrosulfonazo III, and Reactive Black 5-were more efficiently decolorized by PsLac1 in the presence of the redox mediator 1-hydroxybenzotriazole (HBT). Activities were compared in saline conditions, and PsLac2 seemed more adapted to the presence of sea salt than PsLac1. The overall surface charges of the predicted PsLac three-dimensional models showed large negatively charged surfaces for PsLac2, as found in proteins for marine organisms, and more balanced solvent exposed charges for PsLac1, as seen in proteins from terrestrial organisms.
format Journal
author Saowanee Wikee
Juliette Hatton
Annick Turbé-Doan
Yann Mathieu
Marianne Daou
Anne Lomascolo
Abhishek Kumar
Saisamorn Lumyong
Giuliano Sciara
Craig B. Faulds
Eric Record
author_facet Saowanee Wikee
Juliette Hatton
Annick Turbé-Doan
Yann Mathieu
Marianne Daou
Anne Lomascolo
Abhishek Kumar
Saisamorn Lumyong
Giuliano Sciara
Craig B. Faulds
Eric Record
author_sort Saowanee Wikee
title Characterization and dye decolorization potential of two laccases from the marine-derived fungus Pestalotiopsis sp.
title_short Characterization and dye decolorization potential of two laccases from the marine-derived fungus Pestalotiopsis sp.
title_full Characterization and dye decolorization potential of two laccases from the marine-derived fungus Pestalotiopsis sp.
title_fullStr Characterization and dye decolorization potential of two laccases from the marine-derived fungus Pestalotiopsis sp.
title_full_unstemmed Characterization and dye decolorization potential of two laccases from the marine-derived fungus Pestalotiopsis sp.
title_sort characterization and dye decolorization potential of two laccases from the marine-derived fungus pestalotiopsis sp.
publishDate 2019
url https://www.scopus.com/inward/record.uri?partnerID=HzOxMe3b&scp=85064965131&origin=inward
http://cmuir.cmu.ac.th/jspui/handle/6653943832/65386
_version_ 1681426258980765696