Comparative effects of sex hormone deprivation on the brain of insulin-resistant rats

© 2019 Society for Endocrinology Published by Bioscientifica Ltd. Obese-insulin resistance following chronic high-fat diet consumption led to cognitive decline through several mechanisms. Moreover, sex hormone deprivation, including estrogen and testosterone, could be a causative factor in inducing...

Full description

Saved in:
Bibliographic Details
Main Authors: Jirapas Sripetchwandee, Hiranya Pintana, Piangkwan Sanguanmoo, Chiraphat Boonnag, Wasana Pratchayasakul, Nipon Chattipakorn, Siriporn C. Chattipakorn
Format: Journal
Published: 2019
Subjects:
Online Access:https://www.scopus.com/inward/record.uri?partnerID=HzOxMe3b&scp=85063964195&origin=inward
http://cmuir.cmu.ac.th/jspui/handle/6653943832/65407
Tags: Add Tag
No Tags, Be the first to tag this record!
Institution: Chiang Mai University
id th-cmuir.6653943832-65407
record_format dspace
spelling th-cmuir.6653943832-654072019-08-05T04:41:37Z Comparative effects of sex hormone deprivation on the brain of insulin-resistant rats Jirapas Sripetchwandee Hiranya Pintana Piangkwan Sanguanmoo Chiraphat Boonnag Wasana Pratchayasakul Nipon Chattipakorn Siriporn C. Chattipakorn Biochemistry, Genetics and Molecular Biology Medicine © 2019 Society for Endocrinology Published by Bioscientifica Ltd. Obese-insulin resistance following chronic high-fat diet consumption led to cognitive decline through several mechanisms. Moreover, sex hormone deprivation, including estrogen and testosterone, could be a causative factor in inducing cognitive decline. However, comparative studies on the effects of hormone deprivation on the brain are still lacking. Adult Wistar rats from both genders were operated upon (sham operations or orchiectomies/ovariectomies) and given a normal diet or high-fat diet for 4, 8 and 12 weeks. Blood was collected to determine the metabolic parameters. At the end of the experiments, rats were decapitated and their brains were collected to determine brain mitochondrial function, brain oxidative stress, hippocampal plasticity, insulin-induced long-term depression, dendritic spine density and cognition. We found that male and female rats fed a high-fat diet developed obese-insulin resistance by week 8 and brain defects via elevated brain oxidative stress, brain mitochondrial dysfunction, impaired insulin-induced long-term depression, hippocampal dysplasticity, reduced dendritic spine density and cognitive decline by week 12. In normal diet-fed rats, estrogen deprivation, not testosterone deprivation, induced obese-insulin resistance, oxidative stress, brain mitochondrial dysfunction, impaired insulin-induced long-term depression, hippocampal dysplasticity and reduced dendritic spine density. In high-fat-diet-fed rats, estrogen deprivation, not testosterone deprivation, accelerated and aggravated obese-insulin resistance and brain defects at week 8. In conclusion, estrogen deprivation aggravates brain dysfunction more than testosterone deprivation through increased oxidative stress, brain mitochondrial dysfunction, impaired insulin-induced long-term depression and dendritic spine reduction. These findings may explain clinical reports which show more severe cognitive decline in aging females than males with obese-insulin resistance. 2019-08-05T04:32:55Z 2019-08-05T04:32:55Z 2019-01-01 Journal 14796805 00220795 2-s2.0-85063964195 10.1530/JOE-18-0552 https://www.scopus.com/inward/record.uri?partnerID=HzOxMe3b&scp=85063964195&origin=inward http://cmuir.cmu.ac.th/jspui/handle/6653943832/65407
institution Chiang Mai University
building Chiang Mai University Library
country Thailand
collection CMU Intellectual Repository
topic Biochemistry, Genetics and Molecular Biology
Medicine
spellingShingle Biochemistry, Genetics and Molecular Biology
Medicine
Jirapas Sripetchwandee
Hiranya Pintana
Piangkwan Sanguanmoo
Chiraphat Boonnag
Wasana Pratchayasakul
Nipon Chattipakorn
Siriporn C. Chattipakorn
Comparative effects of sex hormone deprivation on the brain of insulin-resistant rats
description © 2019 Society for Endocrinology Published by Bioscientifica Ltd. Obese-insulin resistance following chronic high-fat diet consumption led to cognitive decline through several mechanisms. Moreover, sex hormone deprivation, including estrogen and testosterone, could be a causative factor in inducing cognitive decline. However, comparative studies on the effects of hormone deprivation on the brain are still lacking. Adult Wistar rats from both genders were operated upon (sham operations or orchiectomies/ovariectomies) and given a normal diet or high-fat diet for 4, 8 and 12 weeks. Blood was collected to determine the metabolic parameters. At the end of the experiments, rats were decapitated and their brains were collected to determine brain mitochondrial function, brain oxidative stress, hippocampal plasticity, insulin-induced long-term depression, dendritic spine density and cognition. We found that male and female rats fed a high-fat diet developed obese-insulin resistance by week 8 and brain defects via elevated brain oxidative stress, brain mitochondrial dysfunction, impaired insulin-induced long-term depression, hippocampal dysplasticity, reduced dendritic spine density and cognitive decline by week 12. In normal diet-fed rats, estrogen deprivation, not testosterone deprivation, induced obese-insulin resistance, oxidative stress, brain mitochondrial dysfunction, impaired insulin-induced long-term depression, hippocampal dysplasticity and reduced dendritic spine density. In high-fat-diet-fed rats, estrogen deprivation, not testosterone deprivation, accelerated and aggravated obese-insulin resistance and brain defects at week 8. In conclusion, estrogen deprivation aggravates brain dysfunction more than testosterone deprivation through increased oxidative stress, brain mitochondrial dysfunction, impaired insulin-induced long-term depression and dendritic spine reduction. These findings may explain clinical reports which show more severe cognitive decline in aging females than males with obese-insulin resistance.
format Journal
author Jirapas Sripetchwandee
Hiranya Pintana
Piangkwan Sanguanmoo
Chiraphat Boonnag
Wasana Pratchayasakul
Nipon Chattipakorn
Siriporn C. Chattipakorn
author_facet Jirapas Sripetchwandee
Hiranya Pintana
Piangkwan Sanguanmoo
Chiraphat Boonnag
Wasana Pratchayasakul
Nipon Chattipakorn
Siriporn C. Chattipakorn
author_sort Jirapas Sripetchwandee
title Comparative effects of sex hormone deprivation on the brain of insulin-resistant rats
title_short Comparative effects of sex hormone deprivation on the brain of insulin-resistant rats
title_full Comparative effects of sex hormone deprivation on the brain of insulin-resistant rats
title_fullStr Comparative effects of sex hormone deprivation on the brain of insulin-resistant rats
title_full_unstemmed Comparative effects of sex hormone deprivation on the brain of insulin-resistant rats
title_sort comparative effects of sex hormone deprivation on the brain of insulin-resistant rats
publishDate 2019
url https://www.scopus.com/inward/record.uri?partnerID=HzOxMe3b&scp=85063964195&origin=inward
http://cmuir.cmu.ac.th/jspui/handle/6653943832/65407
_version_ 1681426262875176960