Optimization and characterization of red pigment production from an endophytic fungus, Nigrospora aurantiaca CMU-ZY2045, and its potential source of natural dye for use in textile dyeing

© 2019, Springer-Verlag GmbH Germany, part of Springer Nature. Some of the most important natural pigments have been produced from fungi and used for coloring in food, cosmetics, textiles, and pharmaceutical products. Forty-seven isolates of endophytic fungi were isolated from Cinnamomum zeylanicum...

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Main Authors: Nakarin Suwannarach, Jaturong Kumla, Yuzo Nishizaki, Naoki Sugimoto, Jomkwan Meerak, Kenji Matsui, Saisamorn Lumyong
Format: Journal
Published: 2019
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Institution: Chiang Mai University
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spelling th-cmuir.6653943832-654162019-08-05T04:38:23Z Optimization and characterization of red pigment production from an endophytic fungus, Nigrospora aurantiaca CMU-ZY2045, and its potential source of natural dye for use in textile dyeing Nakarin Suwannarach Jaturong Kumla Yuzo Nishizaki Naoki Sugimoto Jomkwan Meerak Kenji Matsui Saisamorn Lumyong Biochemistry, Genetics and Molecular Biology Immunology and Microbiology © 2019, Springer-Verlag GmbH Germany, part of Springer Nature. Some of the most important natural pigments have been produced from fungi and used for coloring in food, cosmetics, textiles, and pharmaceutical products. Forty-seven isolates of endophytic fungi were isolated from Cinnamomum zeylanicum in northern Thailand. Only one isolate, CMU-ZY2045, produced an extracellularly red pigment. This isolate was identified as Nigrospora aurantiaca based on morphological characteristics and the molecular phylogenetic analysis of a combined four loci (large subunit and internal transcribed spacer of ribosomal DNA, β-tubulin, and translation elongation factor 1-alpha genes). The optimum conditions for red pigment production from this fungus were investigated. The results indicated that the highest red pigment yield was observed in the liquid medium containing glucose as a carbon source and yeast extract as a nitrogen source, at a pH value of 5.0 and at 27 °C with shaking for 5 days. The crude red pigment revealed the highest level of solubility in methanol. A fungal red pigment was found to have high stability at temperatures ranging from 20 to 50 °C and pH values at a range of 5.0–6.0. Based on liquid chromatography-mass spectrometry analyses, the red pigment was characterized as bostrycin. The extracted pigment was used for the textile dyeing process. Crude fungal red pigment revealed the highest staining ability in cotton fabrics and displayed excellent fastness to washing, which showing negative cytotoxicity at the concentrations used to cell culture. This is the first report on bostrycin production from N. aurantiaca. 2019-08-05T04:33:06Z 2019-08-05T04:33:06Z 2019-01-01 Journal 14320614 01757598 2-s2.0-85068325485 10.1007/s00253-019-09926-5 https://www.scopus.com/inward/record.uri?partnerID=HzOxMe3b&scp=85068325485&origin=inward http://cmuir.cmu.ac.th/jspui/handle/6653943832/65416
institution Chiang Mai University
building Chiang Mai University Library
country Thailand
collection CMU Intellectual Repository
topic Biochemistry, Genetics and Molecular Biology
Immunology and Microbiology
spellingShingle Biochemistry, Genetics and Molecular Biology
Immunology and Microbiology
Nakarin Suwannarach
Jaturong Kumla
Yuzo Nishizaki
Naoki Sugimoto
Jomkwan Meerak
Kenji Matsui
Saisamorn Lumyong
Optimization and characterization of red pigment production from an endophytic fungus, Nigrospora aurantiaca CMU-ZY2045, and its potential source of natural dye for use in textile dyeing
description © 2019, Springer-Verlag GmbH Germany, part of Springer Nature. Some of the most important natural pigments have been produced from fungi and used for coloring in food, cosmetics, textiles, and pharmaceutical products. Forty-seven isolates of endophytic fungi were isolated from Cinnamomum zeylanicum in northern Thailand. Only one isolate, CMU-ZY2045, produced an extracellularly red pigment. This isolate was identified as Nigrospora aurantiaca based on morphological characteristics and the molecular phylogenetic analysis of a combined four loci (large subunit and internal transcribed spacer of ribosomal DNA, β-tubulin, and translation elongation factor 1-alpha genes). The optimum conditions for red pigment production from this fungus were investigated. The results indicated that the highest red pigment yield was observed in the liquid medium containing glucose as a carbon source and yeast extract as a nitrogen source, at a pH value of 5.0 and at 27 °C with shaking for 5 days. The crude red pigment revealed the highest level of solubility in methanol. A fungal red pigment was found to have high stability at temperatures ranging from 20 to 50 °C and pH values at a range of 5.0–6.0. Based on liquid chromatography-mass spectrometry analyses, the red pigment was characterized as bostrycin. The extracted pigment was used for the textile dyeing process. Crude fungal red pigment revealed the highest staining ability in cotton fabrics and displayed excellent fastness to washing, which showing negative cytotoxicity at the concentrations used to cell culture. This is the first report on bostrycin production from N. aurantiaca.
format Journal
author Nakarin Suwannarach
Jaturong Kumla
Yuzo Nishizaki
Naoki Sugimoto
Jomkwan Meerak
Kenji Matsui
Saisamorn Lumyong
author_facet Nakarin Suwannarach
Jaturong Kumla
Yuzo Nishizaki
Naoki Sugimoto
Jomkwan Meerak
Kenji Matsui
Saisamorn Lumyong
author_sort Nakarin Suwannarach
title Optimization and characterization of red pigment production from an endophytic fungus, Nigrospora aurantiaca CMU-ZY2045, and its potential source of natural dye for use in textile dyeing
title_short Optimization and characterization of red pigment production from an endophytic fungus, Nigrospora aurantiaca CMU-ZY2045, and its potential source of natural dye for use in textile dyeing
title_full Optimization and characterization of red pigment production from an endophytic fungus, Nigrospora aurantiaca CMU-ZY2045, and its potential source of natural dye for use in textile dyeing
title_fullStr Optimization and characterization of red pigment production from an endophytic fungus, Nigrospora aurantiaca CMU-ZY2045, and its potential source of natural dye for use in textile dyeing
title_full_unstemmed Optimization and characterization of red pigment production from an endophytic fungus, Nigrospora aurantiaca CMU-ZY2045, and its potential source of natural dye for use in textile dyeing
title_sort optimization and characterization of red pigment production from an endophytic fungus, nigrospora aurantiaca cmu-zy2045, and its potential source of natural dye for use in textile dyeing
publishDate 2019
url https://www.scopus.com/inward/record.uri?partnerID=HzOxMe3b&scp=85068325485&origin=inward
http://cmuir.cmu.ac.th/jspui/handle/6653943832/65416
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