Proteomes of the female salivary glands of Simulium nigrogilvum and Simulium nodosum, the main human-biting black flies in Thailand

© 2019 Elsevier B.V. Although several studies have reported pharmacological and immunological activity, as well as the role of black flies in transmitting pathogens to vertebrate hosts through salivary glands (SG) during blood feeding, SG proteomes of the anthropophilic black flies in Thailand have...

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Main Authors: Chayanit Hempolchom, Onrapak Reamtong, Nitat Sookrung, Wichai Srisuka, Yuwaporn Sakolvaree, Wanpen Chaicumpa, Kritsana Taai, Watcharatip Dedkhad, Narissara Jariyapan, Hiroyuki Takaoka, Atiporn Saeung
Format: Journal
Published: 2019
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http://cmuir.cmu.ac.th/jspui/handle/6653943832/65647
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Institution: Chiang Mai University
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spelling th-cmuir.6653943832-656472019-08-05T04:40:23Z Proteomes of the female salivary glands of Simulium nigrogilvum and Simulium nodosum, the main human-biting black flies in Thailand Chayanit Hempolchom Onrapak Reamtong Nitat Sookrung Wichai Srisuka Yuwaporn Sakolvaree Wanpen Chaicumpa Kritsana Taai Watcharatip Dedkhad Narissara Jariyapan Hiroyuki Takaoka Atiporn Saeung Immunology and Microbiology Medicine © 2019 Elsevier B.V. Although several studies have reported pharmacological and immunological activity, as well as the role of black flies in transmitting pathogens to vertebrate hosts through salivary glands (SG) during blood feeding, SG proteomes of the anthropophilic black flies in Thailand have never been reported. Therefore, this study determined the SG proteomes of female S. nigrogilvum and S. nodosum. Sodium dodecylsulfate polyacrylamide gel electrophoresis (SDS-PAGE) and two-dimensional (2-DE) gels containing separated SG proteins of individual species were subjected to liquid chromatography-tandem mass spectrometry (LC–MS/MS) and an orthologous protein search from eukaryotic organism, nematocera and simuliidae databases for total protein identification. SDS-PAGE and protein staining revealed at least 13 and 9 major protein bands in the SGs of female S. nigrogilvum and S. nodosum, respectively, as well as several minor ones. The 2-DE demonstrated a total of 56 and 41 protein spots for S. nigrogilvum and S. nodosum, respectively. Most of the proteins obtained in both species were enzymes involved in blood feeding, including proteases, apyrases, hyaluronidases, aminopeptidase and elastase. The results obtained in this study provided a new body of knowledge for a better understanding on the role of salivary gland proteins in these black fly species in Thailand. 2019-08-05T04:38:08Z 2019-08-05T04:38:08Z 2019-06-01 Journal 18736254 0001706X 2-s2.0-85063620071 10.1016/j.actatropica.2019.03.026 https://www.scopus.com/inward/record.uri?partnerID=HzOxMe3b&scp=85063620071&origin=inward http://cmuir.cmu.ac.th/jspui/handle/6653943832/65647
institution Chiang Mai University
building Chiang Mai University Library
country Thailand
collection CMU Intellectual Repository
topic Immunology and Microbiology
Medicine
spellingShingle Immunology and Microbiology
Medicine
Chayanit Hempolchom
Onrapak Reamtong
Nitat Sookrung
Wichai Srisuka
Yuwaporn Sakolvaree
Wanpen Chaicumpa
Kritsana Taai
Watcharatip Dedkhad
Narissara Jariyapan
Hiroyuki Takaoka
Atiporn Saeung
Proteomes of the female salivary glands of Simulium nigrogilvum and Simulium nodosum, the main human-biting black flies in Thailand
description © 2019 Elsevier B.V. Although several studies have reported pharmacological and immunological activity, as well as the role of black flies in transmitting pathogens to vertebrate hosts through salivary glands (SG) during blood feeding, SG proteomes of the anthropophilic black flies in Thailand have never been reported. Therefore, this study determined the SG proteomes of female S. nigrogilvum and S. nodosum. Sodium dodecylsulfate polyacrylamide gel electrophoresis (SDS-PAGE) and two-dimensional (2-DE) gels containing separated SG proteins of individual species were subjected to liquid chromatography-tandem mass spectrometry (LC–MS/MS) and an orthologous protein search from eukaryotic organism, nematocera and simuliidae databases for total protein identification. SDS-PAGE and protein staining revealed at least 13 and 9 major protein bands in the SGs of female S. nigrogilvum and S. nodosum, respectively, as well as several minor ones. The 2-DE demonstrated a total of 56 and 41 protein spots for S. nigrogilvum and S. nodosum, respectively. Most of the proteins obtained in both species were enzymes involved in blood feeding, including proteases, apyrases, hyaluronidases, aminopeptidase and elastase. The results obtained in this study provided a new body of knowledge for a better understanding on the role of salivary gland proteins in these black fly species in Thailand.
format Journal
author Chayanit Hempolchom
Onrapak Reamtong
Nitat Sookrung
Wichai Srisuka
Yuwaporn Sakolvaree
Wanpen Chaicumpa
Kritsana Taai
Watcharatip Dedkhad
Narissara Jariyapan
Hiroyuki Takaoka
Atiporn Saeung
author_facet Chayanit Hempolchom
Onrapak Reamtong
Nitat Sookrung
Wichai Srisuka
Yuwaporn Sakolvaree
Wanpen Chaicumpa
Kritsana Taai
Watcharatip Dedkhad
Narissara Jariyapan
Hiroyuki Takaoka
Atiporn Saeung
author_sort Chayanit Hempolchom
title Proteomes of the female salivary glands of Simulium nigrogilvum and Simulium nodosum, the main human-biting black flies in Thailand
title_short Proteomes of the female salivary glands of Simulium nigrogilvum and Simulium nodosum, the main human-biting black flies in Thailand
title_full Proteomes of the female salivary glands of Simulium nigrogilvum and Simulium nodosum, the main human-biting black flies in Thailand
title_fullStr Proteomes of the female salivary glands of Simulium nigrogilvum and Simulium nodosum, the main human-biting black flies in Thailand
title_full_unstemmed Proteomes of the female salivary glands of Simulium nigrogilvum and Simulium nodosum, the main human-biting black flies in Thailand
title_sort proteomes of the female salivary glands of simulium nigrogilvum and simulium nodosum, the main human-biting black flies in thailand
publishDate 2019
url https://www.scopus.com/inward/record.uri?partnerID=HzOxMe3b&scp=85063620071&origin=inward
http://cmuir.cmu.ac.th/jspui/handle/6653943832/65647
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