Amycolatopsis samaneae sp. nov., isolated from roots of Samanea saman (Jacq.) Merr
A novel actinomycete, designated strain RM287T, was isolated from surface-sterilized roots of Samanea saman (Jacq.) Merr., collected from Bangkok, Thailand. The status of the novel strain was determined using a polyphasic taxonomic approach. Phylogenetic analyses based on 16S rRNA gene sequences sho...
Saved in:
| Main Authors: | , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
2014
|
| Online Access: | http://www.scopus.com/inward/record.url?eid=2-s2.0-79955421668&partnerID=40&md5=6ce1483b93f94520865fca7abfef902d http://cmuir.cmu.ac.th/handle/6653943832/6581 |
| Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
| Institution: | Chiang Mai University |
| Language: | English |
| Summary: | A novel actinomycete, designated strain RM287T, was isolated from surface-sterilized roots of Samanea saman (Jacq.) Merr., collected from Bangkok, Thailand. The status of the novel strain was determined using a polyphasic taxonomic approach. Phylogenetic analyses based on 16S rRNA gene sequences showed that the organism formed a distinct phyletic line within the radiation of the genus Amycolatopsis. The 16S rRNA gene sequence similarity indicated that strain RM287T was most closely related to Amycolatopsis mediterranei IMSNU 20056T (97.4%), A. rifamycinica DSM 46095T (97.2%), A. kentuckyensis NRRL B-24129T (97.2%), A. pretoriensis DSM 44654T (97%) and A. australiensis DSM 44671T (97%). The novel organism was found to have chemical properties typical of members of the genus Amycolatopsis such as meso-diaminopimelic acid as the dignostic diamino acid in the cell-wall peptidoglycan and arabinose and galactose as the diagnostic sugars. The major menaquinone was MK-9(H4). The major fatty acids were iso-C16: 0 iso-C15: 0, iso 2-OH-C16: 0 and iso-C17: 0. The DNA G+C content was 71.7 mol%. Phenotypic data clearly distinguished the novel isolate from its closest relatives. The combined genotypic and phenotypic data indicated that strain RM287T represented a novel species of the genus Amycolatopsis. The proposed name for this organism is Amycolatopsis samaneae sp. nov., with the type strain RM287T (5TISTR 1919T5BCC 35842T5NBRC 106095T). © G 2011 IUMS. |
|---|