Kinetics of Ultrasound-assisted Extraction of Flavonoids and Triterpenes and Structure Characterization of Chinese Northeast Black Bee Propolis

Flavonoids and triterpenes from Chinese northeast black bee propolis were extracted using pulsed ultrasound-assisted extraction. The influences of extraction parameters such as ethanol concentration, liquid-solid ratio, temperature, time, and ultrasonic on-time/off-time on the extraction yield were...

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Main Authors: Qingzhi Ding, Ricardo Antonio Wu-Chen, Qi Wu, Hanfei Jiang, Ting Zhang, Lin Luo, Haile Ma, Shiqiao Ma, Ronghai He
Language:English
Published: Science Faculty of Chiang Mai University 2019
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Online Access:http://it.science.cmu.ac.th/ejournal/dl.php?journal_id=9777
http://cmuir.cmu.ac.th/jspui/handle/6653943832/66009
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spelling th-cmuir.6653943832-660092019-08-21T09:18:19Z Kinetics of Ultrasound-assisted Extraction of Flavonoids and Triterpenes and Structure Characterization of Chinese Northeast Black Bee Propolis Qingzhi Ding Ricardo Antonio Wu-Chen Qi Wu Hanfei Jiang Ting Zhang Lin Luo Haile Ma Shiqiao Ma Ronghai He propolis ultrasound-assisted extraction kinetics flavonoids triterpenes Flavonoids and triterpenes from Chinese northeast black bee propolis were extracted using pulsed ultrasound-assisted extraction. The influences of extraction parameters such as ethanol concentration, liquid-solid ratio, temperature, time, and ultrasonic on-time/off-time on the extraction yield were investigated. The kinetics of the extraction process was studied. The second-order kinetic models were applied to fit the experimental data. Effects of ultrasound on microstructure of propolis were studied by Scanning Electron Microscope and Atomic Force Microscope. The results showed the great influence of extraction parameters on the extraction yield. Optimum extraction conditions were determined as: 80% ethanol concentration, 30:1 mL/g ratio, 53 °C, 16 min and 10s/5s on-time/off-time. Under these conditions, 60.34% of flavonoids and triterpenes extraction yield was achieved. Scanning electron microscope and atomic force microscope analysis indicated that the propolis matrix structure changed evidently during ultrasound. GC-MS and LC-MS analyses showed that terpenes and flavonoids content were consistent by both extraction methods, thus UAE resulted to be more efficient compared to conventional extraction by achieving extraction in shorter time. Kinetic predictions for yields distributions of ultrasound-assisted extraction are highly in agreement with the experimental data, which implies that the proposed model can be used to predict the mechanisms of the extraction process. 2019-08-21T09:18:19Z 2019-08-21T09:18:19Z 2019 Chiang Mai Journal of Science 46, 1 (Jan 2019), 72 - 92 0125-2526 http://it.science.cmu.ac.th/ejournal/dl.php?journal_id=9777 http://cmuir.cmu.ac.th/jspui/handle/6653943832/66009 Eng Science Faculty of Chiang Mai University
institution Chiang Mai University
building Chiang Mai University Library
country Thailand
collection CMU Intellectual Repository
language English
topic propolis
ultrasound-assisted extraction
kinetics
flavonoids
triterpenes
spellingShingle propolis
ultrasound-assisted extraction
kinetics
flavonoids
triterpenes
Qingzhi Ding
Ricardo Antonio Wu-Chen
Qi Wu
Hanfei Jiang
Ting Zhang
Lin Luo
Haile Ma
Shiqiao Ma
Ronghai He
Kinetics of Ultrasound-assisted Extraction of Flavonoids and Triterpenes and Structure Characterization of Chinese Northeast Black Bee Propolis
description Flavonoids and triterpenes from Chinese northeast black bee propolis were extracted using pulsed ultrasound-assisted extraction. The influences of extraction parameters such as ethanol concentration, liquid-solid ratio, temperature, time, and ultrasonic on-time/off-time on the extraction yield were investigated. The kinetics of the extraction process was studied. The second-order kinetic models were applied to fit the experimental data. Effects of ultrasound on microstructure of propolis were studied by Scanning Electron Microscope and Atomic Force Microscope. The results showed the great influence of extraction parameters on the extraction yield. Optimum extraction conditions were determined as: 80% ethanol concentration, 30:1 mL/g ratio, 53 °C, 16 min and 10s/5s on-time/off-time. Under these conditions, 60.34% of flavonoids and triterpenes extraction yield was achieved. Scanning electron microscope and atomic force microscope analysis indicated that the propolis matrix structure changed evidently during ultrasound. GC-MS and LC-MS analyses showed that terpenes and flavonoids content were consistent by both extraction methods, thus UAE resulted to be more efficient compared to conventional extraction by achieving extraction in shorter time. Kinetic predictions for yields distributions of ultrasound-assisted extraction are highly in agreement with the experimental data, which implies that the proposed model can be used to predict the mechanisms of the extraction process.
author Qingzhi Ding
Ricardo Antonio Wu-Chen
Qi Wu
Hanfei Jiang
Ting Zhang
Lin Luo
Haile Ma
Shiqiao Ma
Ronghai He
author_facet Qingzhi Ding
Ricardo Antonio Wu-Chen
Qi Wu
Hanfei Jiang
Ting Zhang
Lin Luo
Haile Ma
Shiqiao Ma
Ronghai He
author_sort Qingzhi Ding
title Kinetics of Ultrasound-assisted Extraction of Flavonoids and Triterpenes and Structure Characterization of Chinese Northeast Black Bee Propolis
title_short Kinetics of Ultrasound-assisted Extraction of Flavonoids and Triterpenes and Structure Characterization of Chinese Northeast Black Bee Propolis
title_full Kinetics of Ultrasound-assisted Extraction of Flavonoids and Triterpenes and Structure Characterization of Chinese Northeast Black Bee Propolis
title_fullStr Kinetics of Ultrasound-assisted Extraction of Flavonoids and Triterpenes and Structure Characterization of Chinese Northeast Black Bee Propolis
title_full_unstemmed Kinetics of Ultrasound-assisted Extraction of Flavonoids and Triterpenes and Structure Characterization of Chinese Northeast Black Bee Propolis
title_sort kinetics of ultrasound-assisted extraction of flavonoids and triterpenes and structure characterization of chinese northeast black bee propolis
publisher Science Faculty of Chiang Mai University
publishDate 2019
url http://it.science.cmu.ac.th/ejournal/dl.php?journal_id=9777
http://cmuir.cmu.ac.th/jspui/handle/6653943832/66009
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