Identification of Bioactive Molecules from Thai Centipede, Scolopendra subspinipes dehaani, Venom

Centipede venoms are rich sources of bioactive molecules with various actions. These bioactive molecules obtained from Thai centipede, S. subspinipes dehaani, venom have been investigated by proteomic approach and bioactivity assay. Protein profile analysis by SDS-PAGE technique showed 7 major bands...

Full description

Saved in:
Bibliographic Details
Main Authors: Muchalin Meunchan, Nunthawun Uawonggul, Praroonkorn Incamnoi, Sophida Sukprasert, Prapenpuksiri Rungsa, Theerasak Somdee, Sittiruk Roytrakul, Sompong Thammasirirak, Sakda Daduang
Language:English
Published: Science Faculty of Chiang Mai University 2019
Subjects:
Online Access:http://it.science.cmu.ac.th/ejournal/dl.php?journal_id=6507
http://cmuir.cmu.ac.th/jspui/handle/6653943832/66058
Tags: Add Tag
No Tags, Be the first to tag this record!
Institution: Chiang Mai University
Language: English
id th-cmuir.6653943832-66058
record_format dspace
spelling th-cmuir.6653943832-660582019-08-21T09:18:20Z Identification of Bioactive Molecules from Thai Centipede, Scolopendra subspinipes dehaani, Venom Muchalin Meunchan Nunthawun Uawonggul Praroonkorn Incamnoi Sophida Sukprasert Prapenpuksiri Rungsa Theerasak Somdee Sittiruk Roytrakul Sompong Thammasirirak Sakda Daduang bioactive molecules Scolopendrasubspinipesdehaani 2D-PAGE LC-MS/MS Centipede venoms are rich sources of bioactive molecules with various actions. These bioactive molecules obtained from Thai centipede, S. subspinipes dehaani, venom have been investigated by proteomic approach and bioactivity assay. Protein profile analysis by SDS-PAGE technique showed 7 major bands with a molecular weight ranging under 66 kDa. After resolved in 2D-PAGE gel, numerous spots of proteins were found in centipede venom. The trains of major spots were observed at 22 and 45 kDa. The 10 major protein spots were analyzed byliquid chromatography coupled to tandem mass spectrometry(LC-MS/MS). The presence of protease and phospholipase activities in centipede venom was tested by zymographic method andon activity indicator plate (PCY plate), respectively.These molecules were classified into 4 groups including cysteine-rich venom protein, protease, hyaluronidase and phospholipase. 2019-08-21T09:18:20Z 2019-08-21T09:18:20Z 2016 Chiang Mai Journal of Science 43, 1 (Jan 2016), 1 - 10 0125-2526 http://it.science.cmu.ac.th/ejournal/dl.php?journal_id=6507 http://cmuir.cmu.ac.th/jspui/handle/6653943832/66058 Eng Science Faculty of Chiang Mai University
institution Chiang Mai University
building Chiang Mai University Library
country Thailand
collection CMU Intellectual Repository
language English
topic bioactive molecules
Scolopendrasubspinipesdehaani
2D-PAGE
LC-MS/MS
spellingShingle bioactive molecules
Scolopendrasubspinipesdehaani
2D-PAGE
LC-MS/MS
Muchalin Meunchan
Nunthawun Uawonggul
Praroonkorn Incamnoi
Sophida Sukprasert
Prapenpuksiri Rungsa
Theerasak Somdee
Sittiruk Roytrakul
Sompong Thammasirirak
Sakda Daduang
Identification of Bioactive Molecules from Thai Centipede, Scolopendra subspinipes dehaani, Venom
description Centipede venoms are rich sources of bioactive molecules with various actions. These bioactive molecules obtained from Thai centipede, S. subspinipes dehaani, venom have been investigated by proteomic approach and bioactivity assay. Protein profile analysis by SDS-PAGE technique showed 7 major bands with a molecular weight ranging under 66 kDa. After resolved in 2D-PAGE gel, numerous spots of proteins were found in centipede venom. The trains of major spots were observed at 22 and 45 kDa. The 10 major protein spots were analyzed byliquid chromatography coupled to tandem mass spectrometry(LC-MS/MS). The presence of protease and phospholipase activities in centipede venom was tested by zymographic method andon activity indicator plate (PCY plate), respectively.These molecules were classified into 4 groups including cysteine-rich venom protein, protease, hyaluronidase and phospholipase.
author Muchalin Meunchan
Nunthawun Uawonggul
Praroonkorn Incamnoi
Sophida Sukprasert
Prapenpuksiri Rungsa
Theerasak Somdee
Sittiruk Roytrakul
Sompong Thammasirirak
Sakda Daduang
author_facet Muchalin Meunchan
Nunthawun Uawonggul
Praroonkorn Incamnoi
Sophida Sukprasert
Prapenpuksiri Rungsa
Theerasak Somdee
Sittiruk Roytrakul
Sompong Thammasirirak
Sakda Daduang
author_sort Muchalin Meunchan
title Identification of Bioactive Molecules from Thai Centipede, Scolopendra subspinipes dehaani, Venom
title_short Identification of Bioactive Molecules from Thai Centipede, Scolopendra subspinipes dehaani, Venom
title_full Identification of Bioactive Molecules from Thai Centipede, Scolopendra subspinipes dehaani, Venom
title_fullStr Identification of Bioactive Molecules from Thai Centipede, Scolopendra subspinipes dehaani, Venom
title_full_unstemmed Identification of Bioactive Molecules from Thai Centipede, Scolopendra subspinipes dehaani, Venom
title_sort identification of bioactive molecules from thai centipede, scolopendra subspinipes dehaani, venom
publisher Science Faculty of Chiang Mai University
publishDate 2019
url http://it.science.cmu.ac.th/ejournal/dl.php?journal_id=6507
http://cmuir.cmu.ac.th/jspui/handle/6653943832/66058
_version_ 1681426384349560832