Performance of a Gold Electrode Modified with a Nickel Oxy-hydroxide Film for Amperometric Determination of Ethyl Glucuronide, a Biomarker for Alcohol Consumption

• Main Authors: Angkana Meechaichana, Chongdee Thammakhet, Warakorn Limbut, Panote Thavarungkul
• Language: English
• Published: Science Faculty of Chiang Mai University 2019
• Subjects: ethyl glucuronide; biomarker; electrochemical detection; alcohol drinking; nickel modified gold electrode
• Online Access: http://it.science.cmu.ac.th/ejournal/dl.php?journal_id=6309; http://cmuir.cmu.ac.th/jspui/handle/6653943832/66069
• Institution: Chiang Mai University

Ethyl glucuronide (EtG) is a specific and sensitive biomarker of alcohol consumption. It can be detected in urine up to 80 h after the elimination of alcohol from the body. Electrochemical detection based on a gold electrode modified with a nickel oxy-hydroxide film was developed for EtG. This method was simple and produced a rapid result that had an excellent linearity for the determination of EtG, 0.04-30.0 mg L-1 (R2 = 0.996). The limit of detection (LOD) was 0.04 mg L-1 and the limit of quantification (LOQ) was 0.10 mg L-1. The recoveries of the spiked EtG in urine blank samples were in the range of 78±8-119±3%.