Effect of NaCl concentration on Production of Actinomycin-D by Streptomyces sp. Strain J6.2 Isolated from Thailand

Effect of NaCl concentration on Production of Actinomycin-D by Streptomyces sp. Strain J6.2 Isolated from Thailand

Antimicrobial actinomycetes were screened from mangrove soil in Thailand. Streptomyces sp. J6.2 which had high activity and broad spectrum was selected to study it active metabolite. Morphology and physiology of Strain J6.2 indicated a Streptomyces sp. The 16S rDNA sequence for 1,401 bp is closely r...

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Bibliographic Details
Main Authors: Nattachai Kengpipat, Surachai Pornpakakul, Jittra Piapukiew, Thanapat Palaga, Geoff Robson, Anthony J.S. Whalley, Prakitsin Sihanonth
Language:English
Published: Science Faculty of Chiang Mai University 2019
Subjects:
Streptomyces parvulus
actinomycin-D
salt stress
specific productivity
Online Access:http://it.science.cmu.ac.th/ejournal/dl.php?journal_id=6826
http://cmuir.cmu.ac.th/jspui/handle/6653943832/66126
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Institution: Chiang Mai University
Language: English
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Summary:Antimicrobial actinomycetes were screened from mangrove soil in Thailand. Streptomyces sp. J6.2 which had high activity and broad spectrum was selected to study it active metabolite. Morphology and physiology of Strain J6.2 indicated a Streptomyces sp. The 16S rDNA sequence for 1,401 bp is closely related to S. parvulus (100%). A bioactive compound produced by Strain 6.2 was characterized by 2D NMR including COSY, HSQC and HMBC as actinomycin-D. The specific productivity of actinomycin-D was optimized by varying NaCl concentration in culture media. High actinomycin-D yields were obtained in sodium caseinate broth supplemented with 20 g/l malt extract and 20g/l NaCl (80.39 ± 0.72 mg/l).

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