Optimization of Callus Induction and Plant Regeneration in Sugarcane (Saccharum spp.) for a Study of Sucrose Accumulation in Relation to Soluble Acid Invertase Expression

• Main Authors: Trin Srivong, Yun Judy Zhu, Paweena Pongdontri, Upsorn Pliansinchai, Suchirat Saku, Dulal Borthakur, Chifumi Nagai, Manit Kosittrakun
• Language: English
• Published: Science Faculty of Chiang Mai University 2019
• Subjects: sugarcane; callus induction; plant regeneration; soluble acid invertase; sucrose accumulation
• Online Access: http://it.science.cmu.ac.th/ejournal/dl.php?journal_id=6234; http://cmuir.cmu.ac.th/jspui/handle/6653943832/66151
• Institution: Chiang Mai University

The response of three sugarcane genotypes with differential sucrose accumulation to MS medium + 2, 4-dichlorophenoxyacetic acid (2, 4-D) and/or kinetin (kn) was investigated. On MS + 2, 4-D (2 mg/l) + kn (0.5 mg/l), Louisiana Purple (LAP) had the highest callus induction (95.80 %), followed by H65-7...