Endophytic beauveria bassiana in foliar-treated citrus limon plants acting as a growth suppressor to three successive generations of diaphorina citri kuwayama (Hemiptera: Liviidae)

© 2019 by the authors. Licensee MDPI, Basel, Switzerland. Entomopathogenic fungi are commonly applied as inundative sprays to protect plants against insect pests. Their artificial establishment as fungal endophytes to provide other benefits to the host plants aside mere protection against the primar...

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Main Authors: Bamisope Steve Bamisile, Chandra Kanta Dash, Komivi Senyo Akutse, Muhammad Qasim, Luis Carlos Ramos Aguila, Fangfei Wang, Ravindran Keppanan, Liande Wang
Format: Journal
Published: 2019
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http://cmuir.cmu.ac.th/jspui/handle/6653943832/66574
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Institution: Chiang Mai University
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spelling th-cmuir.6653943832-665742019-09-16T12:46:59Z Endophytic beauveria bassiana in foliar-treated citrus limon plants acting as a growth suppressor to three successive generations of diaphorina citri kuwayama (Hemiptera: Liviidae) Bamisope Steve Bamisile Chandra Kanta Dash Komivi Senyo Akutse Muhammad Qasim Luis Carlos Ramos Aguila Fangfei Wang Ravindran Keppanan Liande Wang Agricultural and Biological Sciences © 2019 by the authors. Licensee MDPI, Basel, Switzerland. Entomopathogenic fungi are commonly applied as inundative sprays to protect plants against insect pests. Their artificial establishment as fungal endophytes to provide other benefits to the host plants aside mere protection against the primary pests has also been widely demonstrated. In the present study, two fungal strains of Beauveria bassiana and one strain of Isaria fumosorosea were assessed in a pathogenicity test against adults of Asian citrus psyllid (Diaphorina citri) and found to induce 50% reduction in the survival rate of D. citri adults within 5 days of exposure. The ability of the three fungal strains to endophytically colonize Citrus limon, the impact on plant growth and the effects of systemic colonization on 3 successive generations of D. citri feeding on colonized plants was evaluated. Citrus seedlings at 4 months post-planting were inoculated with each of the fungal strains via foliar spraying. Both strains of B. bassiana successfully colonized the seedlings. One of the B. bassiana strains (BB Fafu-13) was sustained up to 12 weeks in the colonized seedlings, whereas the other B. bassiana strain (BB Fafu-16) was only recovered up to 8 weeks post-inoculation. Isaria fumosorosea (IF Fafu-1) failed to colonize the plant. Both strains of B. bassiana induced significant improvement in plant height and flush production in endophytically colonized seedlings. In addition, endophytic B. bassiana caused 10–15% D. citri adult mortality within 7 days of exposure. Female D. citri feeding on B. bassiana challenged plants laid fewer eggs as compared to those feeding on endophyte-free seedlings, while reduction in adult emergence was recorded on B. bassiana treated plants. With this study, we present the first evidence of B. bassiana artificial establishment as fungal endophyte in citrus plants and its negative effects on D. citri. 2019-09-16T12:46:59Z 2019-09-16T12:46:59Z 2019-01-01 Journal 20754450 2-s2.0-85070213380 10.3390/insects10060176 https://www.scopus.com/inward/record.uri?partnerID=HzOxMe3b&scp=85070213380&origin=inward http://cmuir.cmu.ac.th/jspui/handle/6653943832/66574
institution Chiang Mai University
building Chiang Mai University Library
country Thailand
collection CMU Intellectual Repository
topic Agricultural and Biological Sciences
spellingShingle Agricultural and Biological Sciences
Bamisope Steve Bamisile
Chandra Kanta Dash
Komivi Senyo Akutse
Muhammad Qasim
Luis Carlos Ramos Aguila
Fangfei Wang
Ravindran Keppanan
Liande Wang
Endophytic beauveria bassiana in foliar-treated citrus limon plants acting as a growth suppressor to three successive generations of diaphorina citri kuwayama (Hemiptera: Liviidae)
description © 2019 by the authors. Licensee MDPI, Basel, Switzerland. Entomopathogenic fungi are commonly applied as inundative sprays to protect plants against insect pests. Their artificial establishment as fungal endophytes to provide other benefits to the host plants aside mere protection against the primary pests has also been widely demonstrated. In the present study, two fungal strains of Beauveria bassiana and one strain of Isaria fumosorosea were assessed in a pathogenicity test against adults of Asian citrus psyllid (Diaphorina citri) and found to induce 50% reduction in the survival rate of D. citri adults within 5 days of exposure. The ability of the three fungal strains to endophytically colonize Citrus limon, the impact on plant growth and the effects of systemic colonization on 3 successive generations of D. citri feeding on colonized plants was evaluated. Citrus seedlings at 4 months post-planting were inoculated with each of the fungal strains via foliar spraying. Both strains of B. bassiana successfully colonized the seedlings. One of the B. bassiana strains (BB Fafu-13) was sustained up to 12 weeks in the colonized seedlings, whereas the other B. bassiana strain (BB Fafu-16) was only recovered up to 8 weeks post-inoculation. Isaria fumosorosea (IF Fafu-1) failed to colonize the plant. Both strains of B. bassiana induced significant improvement in plant height and flush production in endophytically colonized seedlings. In addition, endophytic B. bassiana caused 10–15% D. citri adult mortality within 7 days of exposure. Female D. citri feeding on B. bassiana challenged plants laid fewer eggs as compared to those feeding on endophyte-free seedlings, while reduction in adult emergence was recorded on B. bassiana treated plants. With this study, we present the first evidence of B. bassiana artificial establishment as fungal endophyte in citrus plants and its negative effects on D. citri.
format Journal
author Bamisope Steve Bamisile
Chandra Kanta Dash
Komivi Senyo Akutse
Muhammad Qasim
Luis Carlos Ramos Aguila
Fangfei Wang
Ravindran Keppanan
Liande Wang
author_facet Bamisope Steve Bamisile
Chandra Kanta Dash
Komivi Senyo Akutse
Muhammad Qasim
Luis Carlos Ramos Aguila
Fangfei Wang
Ravindran Keppanan
Liande Wang
author_sort Bamisope Steve Bamisile
title Endophytic beauveria bassiana in foliar-treated citrus limon plants acting as a growth suppressor to three successive generations of diaphorina citri kuwayama (Hemiptera: Liviidae)
title_short Endophytic beauveria bassiana in foliar-treated citrus limon plants acting as a growth suppressor to three successive generations of diaphorina citri kuwayama (Hemiptera: Liviidae)
title_full Endophytic beauveria bassiana in foliar-treated citrus limon plants acting as a growth suppressor to three successive generations of diaphorina citri kuwayama (Hemiptera: Liviidae)
title_fullStr Endophytic beauveria bassiana in foliar-treated citrus limon plants acting as a growth suppressor to three successive generations of diaphorina citri kuwayama (Hemiptera: Liviidae)
title_full_unstemmed Endophytic beauveria bassiana in foliar-treated citrus limon plants acting as a growth suppressor to three successive generations of diaphorina citri kuwayama (Hemiptera: Liviidae)
title_sort endophytic beauveria bassiana in foliar-treated citrus limon plants acting as a growth suppressor to three successive generations of diaphorina citri kuwayama (hemiptera: liviidae)
publishDate 2019
url https://www.scopus.com/inward/record.uri?partnerID=HzOxMe3b&scp=85070213380&origin=inward
http://cmuir.cmu.ac.th/jspui/handle/6653943832/66574
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