Goniothalamin induces necroptosis and anoikis in human invasive breast cancer MDA-MB-231 cells
© 2019 by the authors. Licensee MDPI, Basel, Switzerland. Goniothalamin (GTN) is toxic to several types of cancer cells in vitro. However, its effects on non-apoptotic cell death induction of human cancer cells have been poorly documented. Here, an investigation of the anti-cancer activity of GTN an...
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th-cmuir.6653943832-665852019-09-16T12:50:11Z Goniothalamin induces necroptosis and anoikis in human invasive breast cancer MDA-MB-231 cells Patompong Khaw-On Wilart Pompimon Ratana Banjerdpongchai Biochemistry, Genetics and Molecular Biology Chemical Engineering Chemistry Computer Science © 2019 by the authors. Licensee MDPI, Basel, Switzerland. Goniothalamin (GTN) is toxic to several types of cancer cells in vitro. However, its effects on non-apoptotic cell death induction of human cancer cells have been poorly documented. Here, an investigation of the anti-cancer activity of GTN and the molecular signaling pathways of non-apoptotic cell death in the invasive human breast cancer MDA-MB-231 cell line were undertaken. Apoptotic cell death was suppressed by using a pan-caspase inhibitor (Benzyloxycarbonyl-Val-Ala-Asp-[O-methyl]-fluoromethylketone), z-VAD-fmk) as a model to study whether GTN induced caspase-independent cell death. In the anoikis study, MDA-MB-231 cells were cultured on poly-(2-hydroxyethyl methacrylate)-or poly-HEMA-coated plates to mimic anoikis-resistance growth and determine whether GTN induced cell death and the mechanisms involved. GTN and z-VAD-fmk induced human breast cancer MDA-MB-231 cells to undergo necroptosis via endoplasmic reticulum (ER) and oxidative stresses, with increased expressions of necroptotic genes such as rip1, rip3, and mlkl. GTN induced MDA-MB-231 cells to undergo anoikis via reversed epithelial-mesenchymal transition (EMT) protein expressions, inhibited the EGFR/FAK/Src survival signaling pathway, and decreased matrix metalloproteinase secretion. 2019-09-16T12:47:23Z 2019-09-16T12:47:23Z 2019-08-02 Journal 14220067 16616596 2-s2.0-85071497297 10.3390/ijms20163953 https://www.scopus.com/inward/record.uri?partnerID=HzOxMe3b&scp=85071497297&origin=inward http://cmuir.cmu.ac.th/jspui/handle/6653943832/66585 |
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Biochemistry, Genetics and Molecular Biology Chemical Engineering Chemistry Computer Science Patompong Khaw-On Wilart Pompimon Ratana Banjerdpongchai Goniothalamin induces necroptosis and anoikis in human invasive breast cancer MDA-MB-231 cells |
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© 2019 by the authors. Licensee MDPI, Basel, Switzerland. Goniothalamin (GTN) is toxic to several types of cancer cells in vitro. However, its effects on non-apoptotic cell death induction of human cancer cells have been poorly documented. Here, an investigation of the anti-cancer activity of GTN and the molecular signaling pathways of non-apoptotic cell death in the invasive human breast cancer MDA-MB-231 cell line were undertaken. Apoptotic cell death was suppressed by using a pan-caspase inhibitor (Benzyloxycarbonyl-Val-Ala-Asp-[O-methyl]-fluoromethylketone), z-VAD-fmk) as a model to study whether GTN induced caspase-independent cell death. In the anoikis study, MDA-MB-231 cells were cultured on poly-(2-hydroxyethyl methacrylate)-or poly-HEMA-coated plates to mimic anoikis-resistance growth and determine whether GTN induced cell death and the mechanisms involved. GTN and z-VAD-fmk induced human breast cancer MDA-MB-231 cells to undergo necroptosis via endoplasmic reticulum (ER) and oxidative stresses, with increased expressions of necroptotic genes such as rip1, rip3, and mlkl. GTN induced MDA-MB-231 cells to undergo anoikis via reversed epithelial-mesenchymal transition (EMT) protein expressions, inhibited the EGFR/FAK/Src survival signaling pathway, and decreased matrix metalloproteinase secretion. |
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Patompong Khaw-On Wilart Pompimon Ratana Banjerdpongchai |
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Patompong Khaw-On Wilart Pompimon Ratana Banjerdpongchai |
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Patompong Khaw-On |
title |
Goniothalamin induces necroptosis and anoikis in human invasive breast cancer MDA-MB-231 cells |
title_short |
Goniothalamin induces necroptosis and anoikis in human invasive breast cancer MDA-MB-231 cells |
title_full |
Goniothalamin induces necroptosis and anoikis in human invasive breast cancer MDA-MB-231 cells |
title_fullStr |
Goniothalamin induces necroptosis and anoikis in human invasive breast cancer MDA-MB-231 cells |
title_full_unstemmed |
Goniothalamin induces necroptosis and anoikis in human invasive breast cancer MDA-MB-231 cells |
title_sort |
goniothalamin induces necroptosis and anoikis in human invasive breast cancer mda-mb-231 cells |
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2019 |
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https://www.scopus.com/inward/record.uri?partnerID=HzOxMe3b&scp=85071497297&origin=inward http://cmuir.cmu.ac.th/jspui/handle/6653943832/66585 |
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