Bacterial communities in larval diapause and pupal guts in the bamboo borer, omphisa fuscidentalis hampson

• Main Authors: Phakamas Subta, Shun Iwatani, Terd Disayathanoowat, Manaporn Manaboon Poolkeaw, Geoffrey R. Williams, Susumu Kajiwara, Panuwan Chantawannakul
• Format: Journal
• Published: 2019
• Subjects: Biochemistry, Genetics and Molecular Biology; Chemistry; Materials Science; Mathematics; Physics and Astronomy
• Online Access: https://www.scopus.com/inward/record.uri?partnerID=HzOxMe3b&scp=85071165502&origin=inward; http://cmuir.cmu.ac.th/jspui/handle/6653943832/66596
• Institution: Chiang Mai University

© 2019, All Right reserved. This study investigated bacterial diversity in the gut of diapausing larvae and pupae of bamboo borer (Omphisa fuscidentalis) in non-feeding stages by using culture dependent methods. The densities of culturable bacteria in guts of diapausing larvae were high in the foregut and hindgut and it was significantly higher than that from pupae (P < 0.05). Genetic analysis of cultured, isolated bacteria revealed 33 sequences of 16S rRNA genes, from which the bacteria could be divided into three phyla groups, namely Gammaproteobacteria, Firmicutes and Bacteriodes. Current result therefore is the first report of gut bacteria in diapausing larval and pupal stages of O. fuscidentalis. The results indicated that density of bacteria decrease metamorphosis from larvae to pupae and the diversity of bacteria in O. fuscidentalis is similar to many insects gut. Thus, gut bacteria in O. fuscidaentalis may provide larvae digestion and contribute nutrition.