A green colorimetric method using guava leaves extract for quality control of iron content in pharmaceutical formulations

© 2019, Malaysian Society of Analytical Sciences. All rights reserved. A cost-effective and environmentally friendly approach using a simple micro-volume colorimetric method with non-synthetic reagent from plant extracts has been proposed. The crude aqueous extracts from dried guava leaves were util...

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Main Authors: Watsaka Siriangkhawut, Kraingkrai Ponhong, Kate Grudpan
Format: Journal
Published: 2019
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http://cmuir.cmu.ac.th/jspui/handle/6653943832/66624
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Institution: Chiang Mai University
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spelling th-cmuir.6653943832-666242019-09-16T12:49:25Z A green colorimetric method using guava leaves extract for quality control of iron content in pharmaceutical formulations Watsaka Siriangkhawut Kraingkrai Ponhong Kate Grudpan Chemistry © 2019, Malaysian Society of Analytical Sciences. All rights reserved. A cost-effective and environmentally friendly approach using a simple micro-volume colorimetric method with non-synthetic reagent from plant extracts has been proposed. The crude aqueous extracts from dried guava leaves were utilized as an alternative natural reagent for quantification of iron. The method was based on the measurement of a dark brown complex formed by the reaction between Fe(III) and the crude aqueous extracts in acetate buffer pH 4.5 at 562 nm. The optimum conditions for the extraction parameters such as type of solvent, extraction time and mass of dried plant were investigated. Under the optimum conditions, a linear calibration graph in the range of 1.0-10.0 mg L-1 Fe(III) was obtained with limits of detection and quantification of 0.30 and 1.0 mg L-1 Fe(III), respectively. Relative standard deviations of 2.6 and 2.1% were achieved for 2.0 and 4.0 mg L-1 Fe(III) (n=7), respectively. The developed method was successfully applied to quality control of iron in antianemic drug samples. The results are in good agreement with those obtained by the FAAS method at the 95% confidence level. High percentage recoveries between 93 and 99% were obtained. 2019-09-16T12:49:25Z 2019-09-16T12:49:25Z 2019-08-01 Journal 13942506 2-s2.0-85071385039 10.17576/mjas-2019-2304-05 https://www.scopus.com/inward/record.uri?partnerID=HzOxMe3b&scp=85071385039&origin=inward http://cmuir.cmu.ac.th/jspui/handle/6653943832/66624
institution Chiang Mai University
building Chiang Mai University Library
country Thailand
collection CMU Intellectual Repository
topic Chemistry
spellingShingle Chemistry
Watsaka Siriangkhawut
Kraingkrai Ponhong
Kate Grudpan
A green colorimetric method using guava leaves extract for quality control of iron content in pharmaceutical formulations
description © 2019, Malaysian Society of Analytical Sciences. All rights reserved. A cost-effective and environmentally friendly approach using a simple micro-volume colorimetric method with non-synthetic reagent from plant extracts has been proposed. The crude aqueous extracts from dried guava leaves were utilized as an alternative natural reagent for quantification of iron. The method was based on the measurement of a dark brown complex formed by the reaction between Fe(III) and the crude aqueous extracts in acetate buffer pH 4.5 at 562 nm. The optimum conditions for the extraction parameters such as type of solvent, extraction time and mass of dried plant were investigated. Under the optimum conditions, a linear calibration graph in the range of 1.0-10.0 mg L-1 Fe(III) was obtained with limits of detection and quantification of 0.30 and 1.0 mg L-1 Fe(III), respectively. Relative standard deviations of 2.6 and 2.1% were achieved for 2.0 and 4.0 mg L-1 Fe(III) (n=7), respectively. The developed method was successfully applied to quality control of iron in antianemic drug samples. The results are in good agreement with those obtained by the FAAS method at the 95% confidence level. High percentage recoveries between 93 and 99% were obtained.
format Journal
author Watsaka Siriangkhawut
Kraingkrai Ponhong
Kate Grudpan
author_facet Watsaka Siriangkhawut
Kraingkrai Ponhong
Kate Grudpan
author_sort Watsaka Siriangkhawut
title A green colorimetric method using guava leaves extract for quality control of iron content in pharmaceutical formulations
title_short A green colorimetric method using guava leaves extract for quality control of iron content in pharmaceutical formulations
title_full A green colorimetric method using guava leaves extract for quality control of iron content in pharmaceutical formulations
title_fullStr A green colorimetric method using guava leaves extract for quality control of iron content in pharmaceutical formulations
title_full_unstemmed A green colorimetric method using guava leaves extract for quality control of iron content in pharmaceutical formulations
title_sort green colorimetric method using guava leaves extract for quality control of iron content in pharmaceutical formulations
publishDate 2019
url https://www.scopus.com/inward/record.uri?partnerID=HzOxMe3b&scp=85071385039&origin=inward
http://cmuir.cmu.ac.th/jspui/handle/6653943832/66624
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