Neutralizing activity of anti-interferon-γ autoantibodies in adult-onset immunodeficiency is associated with their binding domains

© 2019 Yasamut, Thongkum, Moonmuang, Sakkhachornphop, Chaiwarith, Praparattanapan, Wipasa, Chawansuntati, Supparatpinyo, Lai and Tayapiwatana. Adult-onset immunodeficiency (AOID) with anti-interferon-γ (IFN-γ) autoantibodies (autoAbs) is an emerging immunodeficiency syndrome in Asian countries. The...

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Main Authors: Umpa Yasamut, Weeraya Thongkum, Sutpirat Moonmuang, Supachai Sakkhachornphop, Romanee Chaiwarith, Jutarat Praparattanapan, Jiraprapa Wipasa, Kriangkrai Chawansuntati, Khuanchai Supparatpinyo, Ethan Lai, Chatchai Tayapiwatana
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Published: 2019
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http://cmuir.cmu.ac.th/jspui/handle/6653943832/66685
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Institution: Chiang Mai University
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spelling th-cmuir.6653943832-666852019-09-16T12:58:13Z Neutralizing activity of anti-interferon-γ autoantibodies in adult-onset immunodeficiency is associated with their binding domains Umpa Yasamut Weeraya Thongkum Sutpirat Moonmuang Supachai Sakkhachornphop Romanee Chaiwarith Jutarat Praparattanapan Jiraprapa Wipasa Kriangkrai Chawansuntati Khuanchai Supparatpinyo Ethan Lai Chatchai Tayapiwatana Immunology and Microbiology Medicine © 2019 Yasamut, Thongkum, Moonmuang, Sakkhachornphop, Chaiwarith, Praparattanapan, Wipasa, Chawansuntati, Supparatpinyo, Lai and Tayapiwatana. Adult-onset immunodeficiency (AOID) with anti-interferon-γ (IFN-γ) autoantibodies (autoAbs) is an emerging immunodeficiency syndrome in Asian countries. The presence of neutralizing anti-IFN-γ autoAbs are significantly associated with severe disseminated opportunistic infections. However, the characteristics of the neutralizing antibodies in patients are poorly defined. To better understand the properties of the anti-IFN-γ autoAbs in patients with opportunistic infections, a simplified competitive-binding ELISA was developed. The domains recognized by anti-IFN-γ autoAbs were assessed based on their competition with commercial neutralizing mouse anti-IFN-γ monoclonal antibodies (mAbs). First, the binding affinity and neutralizing capacity of these mAbs (clones B27, B133.5, and MD-1) were characterized. Kinetic analysis and epitope binning using bio-layer interferometry showed the comparable binding affinity of these mAbs to full-length IFN-γ and to the adjacent binding region. These mAbs did not recognize the synthetic 20-mer peptides and inhibited IFN-γ-mediated functions differently. In a competitive-binding ELISA, the anti-IFN-γ autoAbs in AOID serum blocked B27, B133.5, and MD-1 mAb binding. This evidence suggested that the autoAbs that competed with neutralizing mouse anti-IFN-γ mAbs recognized a discontinuous epitope of homodimeric IFN-γ as these mAbs. The patient autoAbs that recognized the B27 epitope exhibited strong neutralizing activity that was determined by the functional analysis. Our results demonstrated the heterogeneity of the autoAbs against IFN-γ in AOID patients and the different patterns among individuals. These data expand upon the fundamental knowledge of neutralizing anti-IFN-γ autoAbs in AOID patients. 2019-09-16T12:53:58Z 2019-09-16T12:53:58Z 2019-01-01 Journal 16643224 2-s2.0-85071756946 10.3389/fimmu.2019.01905 https://www.scopus.com/inward/record.uri?partnerID=HzOxMe3b&scp=85071756946&origin=inward http://cmuir.cmu.ac.th/jspui/handle/6653943832/66685
institution Chiang Mai University
building Chiang Mai University Library
country Thailand
collection CMU Intellectual Repository
topic Immunology and Microbiology
Medicine
spellingShingle Immunology and Microbiology
Medicine
Umpa Yasamut
Weeraya Thongkum
Sutpirat Moonmuang
Supachai Sakkhachornphop
Romanee Chaiwarith
Jutarat Praparattanapan
Jiraprapa Wipasa
Kriangkrai Chawansuntati
Khuanchai Supparatpinyo
Ethan Lai
Chatchai Tayapiwatana
Neutralizing activity of anti-interferon-γ autoantibodies in adult-onset immunodeficiency is associated with their binding domains
description © 2019 Yasamut, Thongkum, Moonmuang, Sakkhachornphop, Chaiwarith, Praparattanapan, Wipasa, Chawansuntati, Supparatpinyo, Lai and Tayapiwatana. Adult-onset immunodeficiency (AOID) with anti-interferon-γ (IFN-γ) autoantibodies (autoAbs) is an emerging immunodeficiency syndrome in Asian countries. The presence of neutralizing anti-IFN-γ autoAbs are significantly associated with severe disseminated opportunistic infections. However, the characteristics of the neutralizing antibodies in patients are poorly defined. To better understand the properties of the anti-IFN-γ autoAbs in patients with opportunistic infections, a simplified competitive-binding ELISA was developed. The domains recognized by anti-IFN-γ autoAbs were assessed based on their competition with commercial neutralizing mouse anti-IFN-γ monoclonal antibodies (mAbs). First, the binding affinity and neutralizing capacity of these mAbs (clones B27, B133.5, and MD-1) were characterized. Kinetic analysis and epitope binning using bio-layer interferometry showed the comparable binding affinity of these mAbs to full-length IFN-γ and to the adjacent binding region. These mAbs did not recognize the synthetic 20-mer peptides and inhibited IFN-γ-mediated functions differently. In a competitive-binding ELISA, the anti-IFN-γ autoAbs in AOID serum blocked B27, B133.5, and MD-1 mAb binding. This evidence suggested that the autoAbs that competed with neutralizing mouse anti-IFN-γ mAbs recognized a discontinuous epitope of homodimeric IFN-γ as these mAbs. The patient autoAbs that recognized the B27 epitope exhibited strong neutralizing activity that was determined by the functional analysis. Our results demonstrated the heterogeneity of the autoAbs against IFN-γ in AOID patients and the different patterns among individuals. These data expand upon the fundamental knowledge of neutralizing anti-IFN-γ autoAbs in AOID patients.
format Journal
author Umpa Yasamut
Weeraya Thongkum
Sutpirat Moonmuang
Supachai Sakkhachornphop
Romanee Chaiwarith
Jutarat Praparattanapan
Jiraprapa Wipasa
Kriangkrai Chawansuntati
Khuanchai Supparatpinyo
Ethan Lai
Chatchai Tayapiwatana
author_facet Umpa Yasamut
Weeraya Thongkum
Sutpirat Moonmuang
Supachai Sakkhachornphop
Romanee Chaiwarith
Jutarat Praparattanapan
Jiraprapa Wipasa
Kriangkrai Chawansuntati
Khuanchai Supparatpinyo
Ethan Lai
Chatchai Tayapiwatana
author_sort Umpa Yasamut
title Neutralizing activity of anti-interferon-γ autoantibodies in adult-onset immunodeficiency is associated with their binding domains
title_short Neutralizing activity of anti-interferon-γ autoantibodies in adult-onset immunodeficiency is associated with their binding domains
title_full Neutralizing activity of anti-interferon-γ autoantibodies in adult-onset immunodeficiency is associated with their binding domains
title_fullStr Neutralizing activity of anti-interferon-γ autoantibodies in adult-onset immunodeficiency is associated with their binding domains
title_full_unstemmed Neutralizing activity of anti-interferon-γ autoantibodies in adult-onset immunodeficiency is associated with their binding domains
title_sort neutralizing activity of anti-interferon-γ autoantibodies in adult-onset immunodeficiency is associated with their binding domains
publishDate 2019
url https://www.scopus.com/inward/record.uri?partnerID=HzOxMe3b&scp=85071756946&origin=inward
http://cmuir.cmu.ac.th/jspui/handle/6653943832/66685
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