Attachment and proliferation of cultured human periodontal ligament cells on 75:25 poly(DL-lactide-CO-ε-caprolactone) electrospun resorbable membrane

75:25 poly(DL-lactide-co-ε-caprolactone) electrospun resorbable membranes were prepared by electrospinning and film casting techniques. Primary cultures of human periodontal ligament (HPDL) fibroblasts cells were seeded on the electrospun membranes, film-cast membranes, and control glass cover slips...

Full description

Saved in:
Bibliographic Details
Main Authors: Phunturug S., Rassameemasmaung S., Punyodom W., Molloy R., Sirikulsathean A., Suchiva K., Piyakunakorn P.
Format: Conference or Workshop Item
Language:English
Published: 2014
Online Access:http://www.scopus.com/inward/record.url?eid=2-s2.0-84860805365&partnerID=40&md5=b7a9b582e9f4eeda4a65e90cca735ce4
http://cmuir.cmu.ac.th/handle/6653943832/6673
Tags: Add Tag
No Tags, Be the first to tag this record!
Institution: Chiang Mai University
Language: English
id th-cmuir.6653943832-6673
record_format dspace
spelling th-cmuir.6653943832-66732014-08-30T03:51:05Z Attachment and proliferation of cultured human periodontal ligament cells on 75:25 poly(DL-lactide-CO-ε-caprolactone) electrospun resorbable membrane Phunturug S. Rassameemasmaung S. Punyodom W. Molloy R. Sirikulsathean A. Suchiva K. Piyakunakorn P. 75:25 poly(DL-lactide-co-ε-caprolactone) electrospun resorbable membranes were prepared by electrospinning and film casting techniques. Primary cultures of human periodontal ligament (HPDL) fibroblasts cells were seeded on the electrospun membranes, film-cast membranes, and control glass cover slips. The number of cell attached was determined at 1, 3, 24 and 72 h after cell seeding. Cell proliferation on the membranes was determined by MTT assay at 1.5 h and 1, 3, 5 and 7 days after cell seeding. The morphology of cells was also determined by SEM. The results indicated that the cell attached number and cell proliferation on the electrospun membranes were significantly higher than that of the film-cast membranes at every time point. From an SEM study, HPDL cells could healthy attach on both, the electrospun membranes and the film-cast membranes. HPDL cells were firmly attached to the membrane matrix in the electrospun membrane group. In the film-cast group, HPDL were loosely attached to the membrane surfaces. In conclusion, an electrospun membrane had better ability to promote HPDL cell attachment and proliferation than did film-cast membranes. © (2012) Trans Tech Publications. 2014-08-30T03:51:05Z 2014-08-30T03:51:05Z 2012 Conference Paper 9.78304E+12 10226680 10.4028/www.scientific.net/AMR.506.114 89735 http://www.scopus.com/inward/record.url?eid=2-s2.0-84860805365&partnerID=40&md5=b7a9b582e9f4eeda4a65e90cca735ce4 http://cmuir.cmu.ac.th/handle/6653943832/6673 English
institution Chiang Mai University
building Chiang Mai University Library
country Thailand
collection CMU Intellectual Repository
language English
description 75:25 poly(DL-lactide-co-ε-caprolactone) electrospun resorbable membranes were prepared by electrospinning and film casting techniques. Primary cultures of human periodontal ligament (HPDL) fibroblasts cells were seeded on the electrospun membranes, film-cast membranes, and control glass cover slips. The number of cell attached was determined at 1, 3, 24 and 72 h after cell seeding. Cell proliferation on the membranes was determined by MTT assay at 1.5 h and 1, 3, 5 and 7 days after cell seeding. The morphology of cells was also determined by SEM. The results indicated that the cell attached number and cell proliferation on the electrospun membranes were significantly higher than that of the film-cast membranes at every time point. From an SEM study, HPDL cells could healthy attach on both, the electrospun membranes and the film-cast membranes. HPDL cells were firmly attached to the membrane matrix in the electrospun membrane group. In the film-cast group, HPDL were loosely attached to the membrane surfaces. In conclusion, an electrospun membrane had better ability to promote HPDL cell attachment and proliferation than did film-cast membranes. © (2012) Trans Tech Publications.
format Conference or Workshop Item
author Phunturug S.
Rassameemasmaung S.
Punyodom W.
Molloy R.
Sirikulsathean A.
Suchiva K.
Piyakunakorn P.
spellingShingle Phunturug S.
Rassameemasmaung S.
Punyodom W.
Molloy R.
Sirikulsathean A.
Suchiva K.
Piyakunakorn P.
Attachment and proliferation of cultured human periodontal ligament cells on 75:25 poly(DL-lactide-CO-ε-caprolactone) electrospun resorbable membrane
author_facet Phunturug S.
Rassameemasmaung S.
Punyodom W.
Molloy R.
Sirikulsathean A.
Suchiva K.
Piyakunakorn P.
author_sort Phunturug S.
title Attachment and proliferation of cultured human periodontal ligament cells on 75:25 poly(DL-lactide-CO-ε-caprolactone) electrospun resorbable membrane
title_short Attachment and proliferation of cultured human periodontal ligament cells on 75:25 poly(DL-lactide-CO-ε-caprolactone) electrospun resorbable membrane
title_full Attachment and proliferation of cultured human periodontal ligament cells on 75:25 poly(DL-lactide-CO-ε-caprolactone) electrospun resorbable membrane
title_fullStr Attachment and proliferation of cultured human periodontal ligament cells on 75:25 poly(DL-lactide-CO-ε-caprolactone) electrospun resorbable membrane
title_full_unstemmed Attachment and proliferation of cultured human periodontal ligament cells on 75:25 poly(DL-lactide-CO-ε-caprolactone) electrospun resorbable membrane
title_sort attachment and proliferation of cultured human periodontal ligament cells on 75:25 poly(dl-lactide-co-ε-caprolactone) electrospun resorbable membrane
publishDate 2014
url http://www.scopus.com/inward/record.url?eid=2-s2.0-84860805365&partnerID=40&md5=b7a9b582e9f4eeda4a65e90cca735ce4
http://cmuir.cmu.ac.th/handle/6653943832/6673
_version_ 1681420657831706624