Production and Immobilization of Levansucrase

• Main Authors: Surawut Sangmanee", Santhana Nakapong, Kamontip Kuttiyawong, Rath Pichyangkura
• Language: English
• Published: Science Faculty of Chiang Mai University 2019
• Subjects: L-FOS; fructooligosaccharide; levansucrase; chitosan; Bacillus licheniformis; Escherichia coli Top-10
• Online Access: http://it.science.cmu.ac.th/ejournal/dl.php?journal_id=5531; http://cmuir.cmu.ac.th/jspui/handle/6653943832/66821
• Institution: Chiang Mai University

Escherichia coli Top-10 containing a levansucrase gene (lsRN) of Bacillus licheniformis RN-01, cultivated in 3X LB medium, produced levansucrase at 65.7 U/ml of culture medium. The purified levansucrase had a MW of 52 kDa and specific activity of 170.04 U/mg protein with 6.6 purification fold and 62...