Isolation and study of thermotolerant Bacillus strains including L-lactic acid production from kitchen refuse
Seven thermotolerant, lactic acid-producing bacteria were isolated from tapioca factory waste and, liquid and solid organic composts in the northeastern Thailand. They were able to grow at temperatures ranging from 30 to 60oC, with maximum growth observed at approximately 42 oC, and within a pH rang...
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Science Faculty of Chiang Mai University
2019
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th-cmuir.6653943832-668222019-09-17T08:55:04Z Isolation and study of thermotolerant Bacillus strains including L-lactic acid production from kitchen refuse Saowanit Tongpim" Kenji Sakai Bacillus fermentation lactic acid kitchen refuse recycling Seven thermotolerant, lactic acid-producing bacteria were isolated from tapioca factory waste and, liquid and solid organic composts in the northeastern Thailand. They were able to grow at temperatures ranging from 30 to 60oC, with maximum growth observed at approximately 42 oC, and within a pH ranging of 5.2 to 7.5, with maximum growth observed at approximately pH 6.5. These bacteria were identified as the genus Bacillus based on their phenotypic characteristics. The fermentation of saccharified kitchen refuse by these strains was also evaluated. Using glucoamylase-pretreated model kitchen refuse as a substrate medium, all seven strains produced L-lactic acid (15.12-24.07 g/l). Among the seven strains tested, strain N15 produced the largest amount of L-lactic acid (24.07 g/l), achieving a 149.3% yield from glucose, 144.9% yield from total sugar, 97% optical activity, 95.5% lactic acid selectivity and 0.31 g/l/h L-lactic acid productivity. By using 16S rDNA sequence analysis, strain N15 displayed 99.45% homology to Bacillus coagulans. 2019-09-17T08:55:04Z 2019-09-17T08:55:04Z 2015 Chiang Mai Journal of Science 42, 1 (Jan 2015), 62 - 69 0125-2526 http://it.science.cmu.ac.th/ejournal/dl.php?journal_id=5522 http://cmuir.cmu.ac.th/jspui/handle/6653943832/66822 Eng Science Faculty of Chiang Mai University |
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Bacillus fermentation lactic acid kitchen refuse recycling |
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Bacillus fermentation lactic acid kitchen refuse recycling Saowanit Tongpim" Kenji Sakai Isolation and study of thermotolerant Bacillus strains including L-lactic acid production from kitchen refuse |
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Seven thermotolerant, lactic acid-producing bacteria were isolated from tapioca factory waste and, liquid and solid organic composts in the northeastern Thailand. They were able to grow at temperatures ranging from 30 to 60oC, with maximum growth observed at approximately 42 oC, and within a pH ranging of 5.2 to 7.5, with maximum growth observed at approximately pH 6.5. These bacteria were identified as the genus Bacillus based on their phenotypic characteristics. The fermentation of saccharified kitchen refuse by these strains was also evaluated. Using glucoamylase-pretreated model kitchen refuse as a substrate medium, all seven strains produced L-lactic acid (15.12-24.07 g/l). Among the seven strains tested, strain N15 produced the largest amount of L-lactic acid (24.07 g/l), achieving a 149.3% yield from glucose, 144.9% yield from total sugar, 97% optical activity, 95.5% lactic acid selectivity and 0.31 g/l/h L-lactic acid productivity. By using 16S rDNA sequence analysis, strain N15 displayed 99.45% homology to Bacillus coagulans. |
author |
Saowanit Tongpim" Kenji Sakai |
author_facet |
Saowanit Tongpim" Kenji Sakai |
author_sort |
Saowanit Tongpim" |
title |
Isolation and study of thermotolerant Bacillus strains including L-lactic acid production from kitchen refuse |
title_short |
Isolation and study of thermotolerant Bacillus strains including L-lactic acid production from kitchen refuse |
title_full |
Isolation and study of thermotolerant Bacillus strains including L-lactic acid production from kitchen refuse |
title_fullStr |
Isolation and study of thermotolerant Bacillus strains including L-lactic acid production from kitchen refuse |
title_full_unstemmed |
Isolation and study of thermotolerant Bacillus strains including L-lactic acid production from kitchen refuse |
title_sort |
isolation and study of thermotolerant bacillus strains including l-lactic acid production from kitchen refuse |
publisher |
Science Faculty of Chiang Mai University |
publishDate |
2019 |
url |
http://it.science.cmu.ac.th/ejournal/dl.php?journal_id=5522 http://cmuir.cmu.ac.th/jspui/handle/6653943832/66822 |
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1681426526663344128 |