Isolation and study of thermotolerant Bacillus strains including L-lactic acid production from kitchen refuse

Seven thermotolerant, lactic acid-producing bacteria were isolated from tapioca factory waste and, liquid and solid organic composts in the northeastern Thailand. They were able to grow at temperatures ranging from 30 to 60oC, with maximum growth observed at approximately 42 oC, and within a pH rang...

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Main Authors: Saowanit Tongpim", Kenji Sakai
Language:English
Published: Science Faculty of Chiang Mai University 2019
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Online Access:http://it.science.cmu.ac.th/ejournal/dl.php?journal_id=5522
http://cmuir.cmu.ac.th/jspui/handle/6653943832/66822
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spelling th-cmuir.6653943832-668222019-09-17T08:55:04Z Isolation and study of thermotolerant Bacillus strains including L-lactic acid production from kitchen refuse Saowanit Tongpim" Kenji Sakai Bacillus fermentation lactic acid kitchen refuse recycling Seven thermotolerant, lactic acid-producing bacteria were isolated from tapioca factory waste and, liquid and solid organic composts in the northeastern Thailand. They were able to grow at temperatures ranging from 30 to 60oC, with maximum growth observed at approximately 42 oC, and within a pH ranging of 5.2 to 7.5, with maximum growth observed at approximately pH 6.5. These bacteria were identified as the genus Bacillus based on their phenotypic characteristics. The fermentation of saccharified kitchen refuse by these strains was also evaluated. Using glucoamylase-pretreated model kitchen refuse as a substrate medium, all seven strains produced L-lactic acid (15.12-24.07 g/l). Among the seven strains tested, strain N15 produced the largest amount of L-lactic acid (24.07 g/l), achieving a 149.3% yield from glucose, 144.9% yield from total sugar, 97% optical activity, 95.5% lactic acid selectivity and 0.31 g/l/h L-lactic acid productivity. By using 16S rDNA sequence analysis, strain N15 displayed 99.45% homology to Bacillus coagulans. 2019-09-17T08:55:04Z 2019-09-17T08:55:04Z 2015 Chiang Mai Journal of Science 42, 1 (Jan 2015), 62 - 69 0125-2526 http://it.science.cmu.ac.th/ejournal/dl.php?journal_id=5522 http://cmuir.cmu.ac.th/jspui/handle/6653943832/66822 Eng Science Faculty of Chiang Mai University
institution Chiang Mai University
building Chiang Mai University Library
country Thailand
collection CMU Intellectual Repository
language English
topic Bacillus
fermentation
lactic acid
kitchen refuse
recycling
spellingShingle Bacillus
fermentation
lactic acid
kitchen refuse
recycling
Saowanit Tongpim"
Kenji Sakai
Isolation and study of thermotolerant Bacillus strains including L-lactic acid production from kitchen refuse
description Seven thermotolerant, lactic acid-producing bacteria were isolated from tapioca factory waste and, liquid and solid organic composts in the northeastern Thailand. They were able to grow at temperatures ranging from 30 to 60oC, with maximum growth observed at approximately 42 oC, and within a pH ranging of 5.2 to 7.5, with maximum growth observed at approximately pH 6.5. These bacteria were identified as the genus Bacillus based on their phenotypic characteristics. The fermentation of saccharified kitchen refuse by these strains was also evaluated. Using glucoamylase-pretreated model kitchen refuse as a substrate medium, all seven strains produced L-lactic acid (15.12-24.07 g/l). Among the seven strains tested, strain N15 produced the largest amount of L-lactic acid (24.07 g/l), achieving a 149.3% yield from glucose, 144.9% yield from total sugar, 97% optical activity, 95.5% lactic acid selectivity and 0.31 g/l/h L-lactic acid productivity. By using 16S rDNA sequence analysis, strain N15 displayed 99.45% homology to Bacillus coagulans.
author Saowanit Tongpim"
Kenji Sakai
author_facet Saowanit Tongpim"
Kenji Sakai
author_sort Saowanit Tongpim"
title Isolation and study of thermotolerant Bacillus strains including L-lactic acid production from kitchen refuse
title_short Isolation and study of thermotolerant Bacillus strains including L-lactic acid production from kitchen refuse
title_full Isolation and study of thermotolerant Bacillus strains including L-lactic acid production from kitchen refuse
title_fullStr Isolation and study of thermotolerant Bacillus strains including L-lactic acid production from kitchen refuse
title_full_unstemmed Isolation and study of thermotolerant Bacillus strains including L-lactic acid production from kitchen refuse
title_sort isolation and study of thermotolerant bacillus strains including l-lactic acid production from kitchen refuse
publisher Science Faculty of Chiang Mai University
publishDate 2019
url http://it.science.cmu.ac.th/ejournal/dl.php?journal_id=5522
http://cmuir.cmu.ac.th/jspui/handle/6653943832/66822
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