Overexpression of OsRab7B3, a small GTP-binding protein gene, enhances leaf senescence in transgenic rice

Rab family proteins are small GTP-binding proteins involved in intracellular trafficking. They play critical roles in several plant development processes. Different expression patterns of 46 Rabs in the rice genome were examined in various rice tissues and in leaves treated with plant growth regulat...

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Main Authors: Pitakrattananukool S., Kawakatsu T., Anuntalabhochai S., Takaiwa F.
Format: Article
Language:English
Published: 2014
Online Access:http://www.scopus.com/inward/record.url?eid=2-s2.0-84864260068&partnerID=40&md5=dd3414a6e7a322cd445f7915d528febe
http://cmuir.cmu.ac.th/handle/6653943832/6748
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Institution: Chiang Mai University
Language: English
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spelling th-cmuir.6653943832-67482014-08-30T03:51:11Z Overexpression of OsRab7B3, a small GTP-binding protein gene, enhances leaf senescence in transgenic rice Pitakrattananukool S. Kawakatsu T. Anuntalabhochai S. Takaiwa F. Rab family proteins are small GTP-binding proteins involved in intracellular trafficking. They play critical roles in several plant development processes. Different expression patterns of 46 Rabs in the rice genome were examined in various rice tissues and in leaves treated with plant growth regulators and under senescence conditions. One of the OsRab genes, OsRab7B3, closely associated with senescence in expression pattern, was chosen for functional analysis. Expression of sGFP under the control of the OsRab7B3 promoter increased in leaves when ABA and NaCl were applied or when kept in dark. In transgenic rice overexpressing OsRab7B3, the senescence-related genes were upregu-lated and leaf senescence was significantly enhanced under dark conditions. Moreover, leaf yellowing occurred earlier in the transgenic plants than in the wild type at the ripening stage. Hence it is suggested that OsRab7B3 act as a stress-inducible gene that plays an important role in the leaf senescence process. 2014-08-30T03:51:11Z 2014-08-30T03:51:11Z 2012 Article 9168451 10.1271/bbb.120050 BBBIE http://www.scopus.com/inward/record.url?eid=2-s2.0-84864260068&partnerID=40&md5=dd3414a6e7a322cd445f7915d528febe http://cmuir.cmu.ac.th/handle/6653943832/6748 English
institution Chiang Mai University
building Chiang Mai University Library
country Thailand
collection CMU Intellectual Repository
language English
description Rab family proteins are small GTP-binding proteins involved in intracellular trafficking. They play critical roles in several plant development processes. Different expression patterns of 46 Rabs in the rice genome were examined in various rice tissues and in leaves treated with plant growth regulators and under senescence conditions. One of the OsRab genes, OsRab7B3, closely associated with senescence in expression pattern, was chosen for functional analysis. Expression of sGFP under the control of the OsRab7B3 promoter increased in leaves when ABA and NaCl were applied or when kept in dark. In transgenic rice overexpressing OsRab7B3, the senescence-related genes were upregu-lated and leaf senescence was significantly enhanced under dark conditions. Moreover, leaf yellowing occurred earlier in the transgenic plants than in the wild type at the ripening stage. Hence it is suggested that OsRab7B3 act as a stress-inducible gene that plays an important role in the leaf senescence process.
format Article
author Pitakrattananukool S.
Kawakatsu T.
Anuntalabhochai S.
Takaiwa F.
spellingShingle Pitakrattananukool S.
Kawakatsu T.
Anuntalabhochai S.
Takaiwa F.
Overexpression of OsRab7B3, a small GTP-binding protein gene, enhances leaf senescence in transgenic rice
author_facet Pitakrattananukool S.
Kawakatsu T.
Anuntalabhochai S.
Takaiwa F.
author_sort Pitakrattananukool S.
title Overexpression of OsRab7B3, a small GTP-binding protein gene, enhances leaf senescence in transgenic rice
title_short Overexpression of OsRab7B3, a small GTP-binding protein gene, enhances leaf senescence in transgenic rice
title_full Overexpression of OsRab7B3, a small GTP-binding protein gene, enhances leaf senescence in transgenic rice
title_fullStr Overexpression of OsRab7B3, a small GTP-binding protein gene, enhances leaf senescence in transgenic rice
title_full_unstemmed Overexpression of OsRab7B3, a small GTP-binding protein gene, enhances leaf senescence in transgenic rice
title_sort overexpression of osrab7b3, a small gtp-binding protein gene, enhances leaf senescence in transgenic rice
publishDate 2014
url http://www.scopus.com/inward/record.url?eid=2-s2.0-84864260068&partnerID=40&md5=dd3414a6e7a322cd445f7915d528febe
http://cmuir.cmu.ac.th/handle/6653943832/6748
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